TCAF1_HUMAN
ID TCAF1_HUMAN Reviewed; 921 AA.
AC Q9Y4C2; A8K6E0; Q75KM8; Q75KM9; Q7L665; Q9BW63;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 3.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=TRPM8 channel-associated factor 1 {ECO:0000303|PubMed:25559186};
DE AltName: Full=TRP channel-associated factor 1 {ECO:0000303|PubMed:25559186};
GN Name=TCAF1 {ECO:0000303|PubMed:25559186, ECO:0000312|HGNC:HGNC:22201};
GN Synonyms=FAM115A, KIAA0738;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=9872452; DOI=10.1093/dnares/5.5.277;
RA Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A.,
RA Kotani H., Nomura N., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XI. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 5:277-286(1998).
RN [2]
RP SEQUENCE REVISION.
RA Ohara O., Suyama M., Nagase T., Ishikawa K., Kikuno R.;
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [8]
RP FUNCTION, INTERACTION WITH TRPM8 AND TRPV6, SUBCELLULAR LOCATION, DOMAIN,
RP AND TISSUE SPECIFICITY.
RX PubMed=25559186; DOI=10.1083/jcb.201402076;
RA Gkika D., Lemonnier L., Shapovalov G., Gordienko D., Poux C.,
RA Bernardini M., Bokhobza A., Bidaux G., Degerny C., Verreman K., Guarmit B.,
RA Benahmed M., de Launoit Y., Bindels R.J., Fiorio Pla A., Prevarskaya N.;
RT "TRP channel-associated factors are a novel protein family that regulates
RT TRPM8 trafficking and activity.";
RL J. Cell Biol. 208:89-107(2015).
CC -!- FUNCTION: Positively regulates the plasma membrane cation channel TRPM8
CC activity. Involved in the recruitment of TRPM8 to the cell surface.
CC Promotes prostate cancer cell migration inhibition in a TRPM8-dependent
CC manner. {ECO:0000269|PubMed:25559186}.
CC -!- SUBUNIT: Interacts with TRPM8 (via N-terminus and C-terminus domains);
CC the interaction inhibits TRPM8 channel activity. Interacts with TRPV6.
CC {ECO:0000269|PubMed:25559186}.
CC -!- INTERACTION:
CC Q9Y4C2; Q9UKT9: IKZF3; NbExp=5; IntAct=EBI-750484, EBI-747204;
CC Q9Y4C2; Q15427: SF3B4; NbExp=5; IntAct=EBI-750484, EBI-348469;
CC Q9Y4C2; O43597: SPRY2; NbExp=3; IntAct=EBI-750484, EBI-742487;
CC Q9Y4C2; P15923: TCF3; NbExp=3; IntAct=EBI-750484, EBI-769630;
CC Q9Y4C2; Q9UBB9: TFIP11; NbExp=3; IntAct=EBI-750484, EBI-1105213;
CC Q9Y4C2; Q15654: TRIP6; NbExp=3; IntAct=EBI-750484, EBI-742327;
CC Q9Y4C2; P17028: ZNF24; NbExp=3; IntAct=EBI-750484, EBI-707773;
CC Q9Y4C2; O14978: ZNF263; NbExp=3; IntAct=EBI-750484, EBI-744493;
CC Q9Y4C2; Q92610: ZNF592; NbExp=3; IntAct=EBI-750484, EBI-1210420;
CC Q9Y4C2-2; Q13490: BIRC2; NbExp=3; IntAct=EBI-11974855, EBI-514538;
CC Q9Y4C2-2; Q9UQM7: CAMK2A; NbExp=3; IntAct=EBI-11974855, EBI-1383687;
CC Q9Y4C2-2; Q8NHQ1: CEP70; NbExp=3; IntAct=EBI-11974855, EBI-739624;
CC Q9Y4C2-2; Q969H4: CNKSR1; NbExp=3; IntAct=EBI-11974855, EBI-741671;
CC Q9Y4C2-2; A8MQ03: CYSRT1; NbExp=3; IntAct=EBI-11974855, EBI-3867333;
CC Q9Y4C2-2; Q9NRI5-2: DISC1; NbExp=3; IntAct=EBI-11974855, EBI-11988027;
CC Q9Y4C2-2; Q9UKT9: IKZF3; NbExp=3; IntAct=EBI-11974855, EBI-747204;
CC Q9Y4C2-2; Q9H2S9: IKZF4; NbExp=3; IntAct=EBI-11974855, EBI-1640423;
CC Q9Y4C2-2; Q8IUG1: KRTAP1-3; NbExp=3; IntAct=EBI-11974855, EBI-11749135;
CC Q9Y4C2-2; P60410: KRTAP10-8; NbExp=3; IntAct=EBI-11974855, EBI-10171774;
CC Q9Y4C2-2; Q52LG2: KRTAP13-2; NbExp=3; IntAct=EBI-11974855, EBI-11953846;
CC Q9Y4C2-2; Q9Y6D9: MAD1L1; NbExp=3; IntAct=EBI-11974855, EBI-742610;
CC Q9Y4C2-2; Q9UJV3-2: MID2; NbExp=3; IntAct=EBI-11974855, EBI-10172526;
CC Q9Y4C2-2; P23511-2: NFYA; NbExp=3; IntAct=EBI-11974855, EBI-11061759;
CC Q9Y4C2-2; P0DPK4: NOTCH2NLC; NbExp=3; IntAct=EBI-11974855, EBI-22310682;
CC Q9Y4C2-2; Q9GZV8: PRDM14; NbExp=3; IntAct=EBI-11974855, EBI-3957793;
CC Q9Y4C2-2; Q9NQX0: PRDM6; NbExp=3; IntAct=EBI-11974855, EBI-11320284;
CC Q9Y4C2-2; Q9NYW8: RBAK; NbExp=3; IntAct=EBI-11974855, EBI-1210429;
CC Q9Y4C2-2; Q8IZE3-2: SCYL3; NbExp=3; IntAct=EBI-11974855, EBI-11959369;
CC Q9Y4C2-2; Q15427: SF3B4; NbExp=3; IntAct=EBI-11974855, EBI-348469;
CC Q9Y4C2-2; Q86VP1: TAX1BP1; NbExp=3; IntAct=EBI-11974855, EBI-529518;
CC Q9Y4C2-2; P15923-3: TCF3; NbExp=3; IntAct=EBI-11974855, EBI-12000326;
CC Q9Y4C2-2; Q9UBB9: TFIP11; NbExp=3; IntAct=EBI-11974855, EBI-1105213;
CC Q9Y4C2-2; Q12933: TRAF2; NbExp=3; IntAct=EBI-11974855, EBI-355744;
CC Q9Y4C2-2; Q8IWZ5: TRIM42; NbExp=3; IntAct=EBI-11974855, EBI-5235829;
CC Q9Y4C2-2; Q9BYV2: TRIM54; NbExp=3; IntAct=EBI-11974855, EBI-2130429;
CC Q9Y4C2-2; P52747: ZNF143; NbExp=3; IntAct=EBI-11974855, EBI-2849334;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:25559186}.
CC Note=Colocalizes with TRPM8 on the plasma membrane.
CC {ECO:0000269|PubMed:25559186}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9Y4C2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9Y4C2-2; Sequence=VSP_031631;
CC -!- TISSUE SPECIFICITY: Isoform 2 is expressed in the prostate and strongly
CC expressed in cancerous prostate samples. {ECO:0000269|PubMed:25559186}.
CC -!- DOMAIN: The C-terminal region is necessary for the channel activity
CC stimulation. {ECO:0000269|PubMed:25559186}.
CC -!- SIMILARITY: Belongs to the TCAF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA34458.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB018281; BAA34458.2; ALT_INIT; mRNA.
DR EMBL; AK291605; BAF84294.1; -; mRNA.
DR EMBL; AC099548; AAQ96852.1; -; Genomic_DNA.
DR EMBL; AC099548; AAQ96853.1; -; Genomic_DNA.
DR EMBL; BC000609; AAH00609.1; -; mRNA.
DR CCDS; CCDS56514.1; -. [Q9Y4C2-2]
DR CCDS; CCDS5886.1; -. [Q9Y4C2-1]
DR RefSeq; NP_001193867.1; NM_001206938.1. [Q9Y4C2-2]
DR RefSeq; NP_001193870.1; NM_001206941.1.
DR RefSeq; NP_055534.1; NM_014719.2. [Q9Y4C2-1]
DR RefSeq; XP_005250131.1; XM_005250074.3. [Q9Y4C2-1]
DR RefSeq; XP_005250132.1; XM_005250075.2. [Q9Y4C2-1]
DR RefSeq; XP_005250133.1; XM_005250076.3. [Q9Y4C2-1]
DR RefSeq; XP_005250134.1; XM_005250077.2. [Q9Y4C2-1]
DR RefSeq; XP_006716254.1; XM_006716191.2. [Q9Y4C2-1]
DR RefSeq; XP_006716255.1; XM_006716192.3. [Q9Y4C2-2]
DR RefSeq; XP_016868322.1; XM_017012833.1. [Q9Y4C2-2]
DR RefSeq; XP_016868323.1; XM_017012834.1.
DR RefSeq; XP_016868324.1; XM_017012835.1.
DR RefSeq; XP_016868325.1; XM_017012836.1.
DR AlphaFoldDB; Q9Y4C2; -.
DR BioGRID; 115095; 127.
DR IntAct; Q9Y4C2; 50.
DR MINT; Q9Y4C2; -.
DR STRING; 9606.ENSP00000419235; -.
DR MEROPS; M98.A03; -.
DR iPTMnet; Q9Y4C2; -.
DR PhosphoSitePlus; Q9Y4C2; -.
DR BioMuta; TCAF1; -.
DR DMDM; 182628294; -.
DR EPD; Q9Y4C2; -.
DR jPOST; Q9Y4C2; -.
DR MassIVE; Q9Y4C2; -.
DR MaxQB; Q9Y4C2; -.
DR PaxDb; Q9Y4C2; -.
DR PeptideAtlas; Q9Y4C2; -.
DR PRIDE; Q9Y4C2; -.
DR ProteomicsDB; 86158; -. [Q9Y4C2-1]
DR ProteomicsDB; 86159; -. [Q9Y4C2-2]
DR Antibodypedia; 66174; 33 antibodies from 11 providers.
DR DNASU; 9747; -.
DR Ensembl; ENST00000355951.2; ENSP00000348220.2; ENSG00000198420.10. [Q9Y4C2-2]
DR Ensembl; ENST00000479870.6; ENSP00000419235.1; ENSG00000198420.10. [Q9Y4C2-1]
DR GeneID; 9747; -.
DR KEGG; hsa:9747; -.
DR MANE-Select; ENST00000479870.6; ENSP00000419235.1; NM_014719.3; NP_055534.2.
DR UCSC; uc003wdo.3; human. [Q9Y4C2-1]
DR CTD; 9747; -.
DR DisGeNET; 9747; -.
DR GeneCards; TCAF1; -.
DR HGNC; HGNC:22201; TCAF1.
DR HPA; ENSG00000198420; Low tissue specificity.
DR MIM; 616251; gene.
DR neXtProt; NX_Q9Y4C2; -.
DR OpenTargets; ENSG00000198420; -.
DR PharmGKB; PA162385749; -.
DR VEuPathDB; HostDB:ENSG00000198420; -.
DR eggNOG; ENOG502S2AP; Eukaryota.
DR GeneTree; ENSGT00390000017365; -.
DR HOGENOM; CLU_011215_0_0_1; -.
DR InParanoid; Q9Y4C2; -.
DR OMA; MQIPSSW; -.
DR OrthoDB; 1049811at2759; -.
DR PhylomeDB; Q9Y4C2; -.
DR TreeFam; TF331520; -.
DR PathwayCommons; Q9Y4C2; -.
DR SignaLink; Q9Y4C2; -.
DR BioGRID-ORCS; 9747; 21 hits in 1071 CRISPR screens.
DR ChiTaRS; TCAF1; human.
DR GenomeRNAi; 9747; -.
DR Pharos; Q9Y4C2; Tbio.
DR PRO; PR:Q9Y4C2; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q9Y4C2; protein.
DR Bgee; ENSG00000198420; Expressed in adrenal tissue and 216 other tissues.
DR ExpressionAtlas; Q9Y4C2; baseline and differential.
DR Genevisible; Q9Y4C2; HS.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0044325; F:transmembrane transporter binding; IDA:MGI.
DR GO; GO:0030336; P:negative regulation of cell migration; IDA:UniProtKB.
DR GO; GO:1901529; P:positive regulation of anion channel activity; IDA:UniProtKB.
DR GO; GO:0090314; P:positive regulation of protein targeting to membrane; IMP:UniProtKB.
DR GO; GO:0010359; P:regulation of anion channel activity; IBA:GO_Central.
DR Gene3D; 1.10.390.30; -; 1.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR035423; M60-like_N.
DR InterPro; IPR042279; Pep_M60_3.
DR InterPro; IPR031161; Peptidase_M60_dom.
DR Pfam; PF17291; M60-like_N; 1.
DR Pfam; PF13402; Peptidase_M60; 1.
DR SMART; SM01276; M60-like; 1.
DR SUPFAM; SSF52317; SSF52317; 1.
DR PROSITE; PS51723; PEPTIDASE_M60; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Membrane; Reference proteome;
KW Transport.
FT CHAIN 1..921
FT /note="TRPM8 channel-associated factor 1"
FT /id="PRO_0000320182"
FT DOMAIN 542..841
FT /note="Peptidase M60"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01060"
FT VAR_SEQ 920..921
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_031631"
FT CONFLICT 301
FT /note="V -> I (in Ref. 1; BAA34458, 3; BAF84294 and 5;
FT AAH00609)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 921 AA; 102126 MW; BE654261858729B4 CRC64;
MATPSAAFEA LMNGVTSWDV PEDAVPCELL LIGEASFPVM VNDMGQVLIA ASSYGRGRLV
VVSHEDYLVE AQLTPFLLNA VGWLCSSPGA PIGVHPSLAP LAKILEGSGV DAKVEPEVKD
SLGVYCIDAY NETMTEKLVK FMKCGGGLLI GGQAWDWANQ GEDERVLFTF PGNLVTSVAG
IYFTDNKGDT SFFKVSKKMP KIPVLVSCED DLSDDREELL HGISELDISN SDCFPSQLLV
HGALAFPLGL DSYHGCVIAA ARYGRGRVVV TGHKVLFTVG KLGPFLLNAV RWLDGGRRGK
VVVQTELRTL SGLLAVGGID TSIEPNLTSD ASVYCFEPVS EVGVKELQEF VAEGGGLFVG
AQAWWWAFKN PGVSPLARFP GNLLLNPFGI SITSQSLNPG PFRTPKAGIR TYHFRSTLAE
FQVIMGRKRG NVEKGWLAKL GPDGAAFLQI PAEEIPAYMS VHRLLRKLLS RYRLPVATRE
NPVINDCCRG AMLSLATGLA HSGSDLSLLV PEIEDMYSSP YLRPSESPIT VEVNCTNPGT
RYCWMSTGLY IPGRQIIEVS LPEAAASADL KIQIGCHTDD LTRASKLFRG PLVINRCCLD
KPTKSITCLW GGLLYIIVPQ NSKLGSVPVT VKGAVHAPYY KLGETTLEEW KRRIQENPGP
WGELATDNII LTVPTANLRT LENPEPLLRL WDEVMQAVAR LGAEPFPLRL PQRIVADVQI
SVGWMHAGYP IMCHLESVQE LINEKLIRTK GLWGPVHELG RNQQRQEWEF PPHTTEATCN
LWCVYVHETV LGIPRSRANI ALWPPVREKR VRIYLSKGPN VKNWNAWTAL ETYLQLQEAF
GWEPFIRLFT EYRNQTNLPT ENVDKMNLWV KMFSHQVQKN LAPFFEAWAW PIQKEVATSL
AYLPEWKENI MKLYLLTQMP H
