TCAL4_HUMAN
ID TCAL4_HUMAN Reviewed; 215 AA.
AC Q96EI5; Q8WY12; Q9H2H1; Q9H775;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Transcription elongation factor A protein-like 4;
DE Short=TCEA-like protein 4;
DE AltName: Full=Transcription elongation factor S-II protein-like 4;
GN Name=TCEAL4; ORFNames=NPD017;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=12905813;
RA Lu X.W., Yin J.Y., Cui L.X.;
RT "Cloning of human B lymphocyte activation-related novel gene.";
RL Zhongguo Yi Xue Ke Xue Yuan Xue Bao 23:27-31(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Pituitary;
RA Xu X., Yang Y., Gao G., Xiao H., Chen Z., Han Z.;
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Smooth muscle;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [9]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6 AND SER-88, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [11]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: May be involved in transcriptional regulation.
CC -!- INTERACTION:
CC Q96EI5; O43933: PEX1; NbExp=3; IntAct=EBI-2511291, EBI-988601;
CC Q96EI5; O76024: WFS1; NbExp=3; IntAct=EBI-2511291, EBI-720609;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q96EI5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96EI5-2; Sequence=VSP_019109;
CC -!- SIMILARITY: Belongs to the TFS-II family. TFA subfamily. {ECO:0000305}.
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DR EMBL; AF314542; AAL40909.1; -; mRNA.
DR EMBL; AF271783; AAG44794.1; -; mRNA.
DR EMBL; AK024827; BAB15023.1; -; mRNA.
DR EMBL; Z73965; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC012296; AAH12296.1; -; mRNA.
DR CCDS; CCDS14510.2; -. [Q96EI5-1]
DR CCDS; CCDS76004.1; -. [Q96EI5-2]
DR RefSeq; NP_001006936.1; NM_001006935.2. [Q96EI5-1]
DR RefSeq; NP_001006938.1; NM_001006937.2. [Q96EI5-1]
DR RefSeq; NP_001287830.1; NM_001300901.1. [Q96EI5-2]
DR RefSeq; NP_001292769.1; NM_001305840.1. [Q96EI5-1]
DR RefSeq; NP_001292770.1; NM_001305841.1. [Q96EI5-1]
DR RefSeq; NP_001292771.1; NM_001305842.1. [Q96EI5-1]
DR RefSeq; NP_079139.4; NM_024863.5. [Q96EI5-1]
DR RefSeq; XP_016885346.1; XM_017029857.1. [Q96EI5-1]
DR AlphaFoldDB; Q96EI5; -.
DR BioGRID; 122998; 28.
DR IntAct; Q96EI5; 18.
DR STRING; 9606.ENSP00000361712; -.
DR iPTMnet; Q96EI5; -.
DR MetOSite; Q96EI5; -.
DR PhosphoSitePlus; Q96EI5; -.
DR BioMuta; TCEAL4; -.
DR DMDM; 108935938; -.
DR EPD; Q96EI5; -.
DR jPOST; Q96EI5; -.
DR MassIVE; Q96EI5; -.
DR MaxQB; Q96EI5; -.
DR PaxDb; Q96EI5; -.
DR PeptideAtlas; Q96EI5; -.
DR PRIDE; Q96EI5; -.
DR ProteomicsDB; 76411; -. [Q96EI5-1]
DR ProteomicsDB; 76412; -. [Q96EI5-2]
DR Antibodypedia; 35108; 72 antibodies from 22 providers.
DR DNASU; 79921; -.
DR Ensembl; ENST00000372629.4; ENSP00000361712.4; ENSG00000133142.19. [Q96EI5-2]
DR Ensembl; ENST00000415568.2; ENSP00000415564.2; ENSG00000133142.19. [Q96EI5-1]
DR Ensembl; ENST00000468024.5; ENSP00000421857.1; ENSG00000133142.19. [Q96EI5-1]
DR Ensembl; ENST00000472484.6; ENSP00000421156.1; ENSG00000133142.19. [Q96EI5-1]
DR Ensembl; ENST00000472745.1; ENSP00000424314.1; ENSG00000133142.19. [Q96EI5-1]
DR Ensembl; ENST00000494801.5; ENSP00000427494.1; ENSG00000133142.19. [Q96EI5-1]
DR GeneID; 79921; -.
DR KEGG; hsa:79921; -.
DR MANE-Select; ENST00000472484.6; ENSP00000421156.1; NM_001006935.3; NP_001006936.1.
DR UCSC; uc004ekl.4; human. [Q96EI5-1]
DR CTD; 79921; -.
DR DisGeNET; 79921; -.
DR GeneCards; TCEAL4; -.
DR HGNC; HGNC:26121; TCEAL4.
DR HPA; ENSG00000133142; Low tissue specificity.
DR neXtProt; NX_Q96EI5; -.
DR OpenTargets; ENSG00000133142; -.
DR PharmGKB; PA128394724; -.
DR VEuPathDB; HostDB:ENSG00000133142; -.
DR eggNOG; ENOG502SXKH; Eukaryota.
DR GeneTree; ENSGT00950000183164; -.
DR HOGENOM; CLU_078412_0_0_1; -.
DR InParanoid; Q96EI5; -.
DR OMA; QGEMEYK; -.
DR OrthoDB; 1366017at2759; -.
DR PhylomeDB; Q96EI5; -.
DR TreeFam; TF336871; -.
DR PathwayCommons; Q96EI5; -.
DR SignaLink; Q96EI5; -.
DR BioGRID-ORCS; 79921; 24 hits in 711 CRISPR screens.
DR ChiTaRS; TCEAL4; human.
DR GeneWiki; TCEAL4; -.
DR GenomeRNAi; 79921; -.
DR Pharos; Q96EI5; Tdark.
DR PRO; PR:Q96EI5; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; Q96EI5; protein.
DR Bgee; ENSG00000133142; Expressed in right ovary and 207 other tissues.
DR ExpressionAtlas; Q96EI5; baseline and differential.
DR Genevisible; Q96EI5; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0050699; F:WW domain binding; IBA:GO_Central.
DR InterPro; IPR021156; TF_A-like/BEX.
DR Pfam; PF04538; BEX; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..215
FT /note="Transcription elongation factor A protein-like 4"
FT /id="PRO_0000239209"
FT REGION 1..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..133
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES 6
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 88
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 102
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT VAR_SEQ 1
FT /note="M -> MPAGGQAPQLTPVIPAFWEARGLPNSKGRDKVHICQCEEWKGHISYV
FT ERDITTSEIKSLPQVSVRAFHAASGTLEWRKGNGYGRGKGWGWGRVESREAGPSVDRDG
FT GLRVCCSQRSAKPEKEEQPVQNPRRSVKDRKRRGNLDM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12905813"
FT /id="VSP_019109"
FT CONFLICT 132
FT /note="K -> T (in Ref. 5; AAH12296)"
FT /evidence="ECO:0000305"
FT CONFLICT 157
FT /note="R -> G (in Ref. 3; BAB15023)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 215 AA; 24647 MW; 53420116CD9A7A0B CRC64;
MEKLYSENEG MASNQGKMEN EEQPQDERKP EVTCTLEDKK LENEGKTENK GKTGDEEMLK
DKGKPESEGE AKEGKSEREG ESEMEGGSER EGKPEIEGKP ESEGEPGSET RAAGKRPAED
DVPRKAKRKT NKGLAHYLKE YKEAIHDMNF SNEDMIREFD NMAKVQDEKR KSKQKLGAFL
WMQRNLQDPF YPRGPREFRG GCRAPRRDIE DIPYV
