ID   TCAR_STAAE              Reviewed;         151 AA.
AC   A6QJJ8;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=HTH-type transcriptional regulator TcaR;
GN   Name=tcaR; OrderedLocusNames=NWMN_2258;
OS   Staphylococcus aureus (strain Newman).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=426430;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Newman;
RX   PubMed=17951380; DOI=10.1128/jb.01000-07;
RA   Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K.;
RT   "Genome sequence of Staphylococcus aureus strain Newman and comparative
RT   analysis of staphylococcal genomes: polymorphism and evolution of two major
RT   pathogenicity islands.";
RL   J. Bacteriol. 190:300-310(2008).
RN   [2]
RP   FUNCTION AS ICA OPERON REPRESSOR, AND DISRUPTION PHENOTYPE.
RX   PubMed=15060048; DOI=10.1128/jb.186.8.2449-2456.2004;
RA   Jefferson K.K., Pier D.B., Goldmann D.A., Pier G.B.;
RT   "The teicoplanin-associated locus regulator (TcaR) and the intercellular
RT   adhesin locus regulator (IcaR) are transcriptional inhibitors of the ica
RT   locus in Staphylococcus aureus.";
RL   J. Bacteriol. 186:2449-2456(2004).
CC   -!- FUNCTION: Involved in the antibiotic teicoplanin susceptibility.
CC       Inactivation of the tcaRAB operon leads to teicoplanin resistance.
CC       {ECO:0000269|PubMed:15060048}.
CC   -!- FUNCTION: Is a weak negative regulator of transcription of the icaADBC
CC       operon necessary for biofilm production. {ECO:0000269|PubMed:15060048}.
CC   -!- DISRUPTION PHENOTYPE: Cells display a 5-fold increase of the ica
CC       transcription but has no significant effect on the adherence ability
CC       nor on the amount of poly-N-acetylglucosamine polysaccharide (PNAG)
CC       produced with respect to the wild-type strain. Double deletion of icaR
CC       and tcaR increases ica transcription about 500-fold, and poly-N-
CC       acetylglucosamine polysaccharide (PNAG) synthesis about 100-fold.
CC       Adherence is significantly greater than that of the single icaR mutant.
CC       {ECO:0000269|PubMed:15060048}.
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DR   EMBL; AP009351; BAF68530.1; -; Genomic_DNA.
DR   RefSeq; WP_000238542.1; NZ_CP023390.1.
DR   AlphaFoldDB; A6QJJ8; -.
DR   SMR; A6QJJ8; -.
DR   EnsemblBacteria; BAF68530; BAF68530; NWMN_2258.
DR   KEGG; sae:NWMN_2258; -.
DR   HOGENOM; CLU_1721241_0_0_9; -.
DR   OMA; LVEWDDC; -.
DR   Proteomes; UP000006386; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   CDD; cd00090; HTH_ARSR; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011991; ArsR-like_HTH.
DR   InterPro; IPR000835; HTH_MarR-typ.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF12802; MarR_2; 1.
DR   SMART; SM00347; HTH_MARR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50995; HTH_MARR_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..151
FT                   /note="HTH-type transcriptional regulator TcaR"
FT                   /id="PRO_0000317037"
FT   DOMAIN          1..142
FT                   /note="HTH marR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00345"
FT   DNA_BIND        54..77
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00345"
SQ   SEQUENCE   151 AA;  17521 MW;  7FEFD9D39F2F6C53 CRC64;
     MVKHLQDHIQ FLEQFINNVN ALTAKMLKDL QNEYEISLEQ SNVLGMLNKE PLTISEITQR
     QGVNKAAVSR RIKKLIDAKL VKLDKPNLNI DQRLKFITLT DKGRAYLKER NAIMTDIAQD
     ITNDLNSEDI ENVRQVLEVI NHRIKTYSNH K