TCBE_PSESQ
ID TCBE_PSESQ Reviewed; 238 AA.
AC P27100;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Carboxymethylenebutenolidase;
DE EC=3.1.1.45;
DE AltName: Full=Dienelactone hydrolase;
DE Short=DLH;
GN Name=tcbE;
OS Pseudomonas sp. (strain P51).
OG Plasmid pP51.
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae.
OX NCBI_TaxID=65067;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2013566; DOI=10.1128/jb.173.8.2425-2434.1991;
RA van der Meer J.R., Eggen R.I., Zehnder A.J., de Vos W.M.;
RT "Sequence analysis of the Pseudomonas sp. strain P51 tcb gene cluster,
RT which encodes metabolism of chlorinated catechols: evidence for
RT specialization of catechol 1,2-dioxygenases for chlorinated substrates.";
RL J. Bacteriol. 173:2425-2434(1991).
CC -!- FUNCTION: Ring cleavage of cyclic ester dienelactone to produce
CC maleylacetate.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC 2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC -!- PATHWAY: Aromatic compound metabolism; 3-chlorocatechol degradation.
CC -!- SUBUNIT: Monomer.
CC -!- MISCELLANEOUS: Carboxymethylenebutenolidase is specific for
CC dienelactone and has no activity toward enol-lactones.
CC -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC {ECO:0000305}.
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DR EMBL; M57629; AAD13628.1; -; Genomic_DNA.
DR PIR; D43673; D43673.
DR AlphaFoldDB; P27100; -.
DR SMR; P27100; -.
DR ESTHER; alceu-CBNC; Dienelactone_hydrolase.
DR BioCyc; MetaCyc:MON-14406; -.
DR UniPathway; UPA00083; -.
DR GO; GO:0008806; F:carboxymethylenebutenolidase activity; IEA:UniProtKB-EC.
DR GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002925; Dienelactn_hydro.
DR Pfam; PF01738; DLH; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 3: Inferred from homology;
KW Aromatic hydrocarbons catabolism; Hydrolase; Plasmid; Serine esterase.
FT CHAIN 1..238
FT /note="Carboxymethylenebutenolidase"
FT /id="PRO_0000161573"
FT ACT_SITE 123
FT /evidence="ECO:0000250"
FT ACT_SITE 171
FT /evidence="ECO:0000250"
FT ACT_SITE 201
FT /evidence="ECO:0000250"
SQ SEQUENCE 238 AA; 25812 MW; DB57EAD5229C03C8 CRC64;
MLTEGLSIDA KGGGRFGAHL QLPARGRGPV VMVAQEIFGV NPFMTEVLAW LASEGFVGLC
PDLYWRHGPG IEFDPNDEVQ RARALGMFRD YKLEDGVADL RATVAYAASQ PFCDGGVAVI
GYCLGGALAY EVAAEGFAQC CVGYYGVGFE KRLERARLVK TPSMFHMGTN DHFVTAEARQ
LITNAFEANP AIALHWYDAG HSFARASSPN FSPEATRTAN ARTLEMLKRM KPIGTIGQ