ID   TCBF_PSESQ              Reviewed;         352 AA.
AC   P27101;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Maleylacetate reductase;
DE            EC=1.3.1.32;
GN   Name=tcbF;
OS   Pseudomonas sp. (strain P51).
OG   Plasmid pP51.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae.
OX   NCBI_TaxID=65067;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2013566; DOI=10.1128/jb.173.8.2425-2434.1991;
RA   van der Meer J.R., Eggen R.I., Zehnder A.J., de Vos W.M.;
RT   "Sequence analysis of the Pseudomonas sp. strain P51 tcb gene cluster,
RT   which encodes metabolism of chlorinated catechols: evidence for
RT   specialization of catechol 1,2-dioxygenases for chlorinated substrates.";
RL   J. Bacteriol. 173:2425-2434(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-oxoadipate + NAD(+) = H(+) + maleylacetate + NADH;
CC         Xref=Rhea:RHEA:16981, ChEBI:CHEBI:15378, ChEBI:CHEBI:15775,
CC         ChEBI:CHEBI:16468, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.3.1.32;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-oxoadipate + NADP(+) = H(+) + maleylacetate + NADPH;
CC         Xref=Rhea:RHEA:16985, ChEBI:CHEBI:15378, ChEBI:CHEBI:15775,
CC         ChEBI:CHEBI:16468, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.3.1.32;
CC   -!- PATHWAY: Aromatic compound metabolism; 3-chlorocatechol degradation.
CC   -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC       family. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to be a trans-dienelactoneisomerase.
CC       {ECO:0000305|PubMed:2013566}.
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DR   EMBL; M57629; AAD13629.1; -; Genomic_DNA.
DR   PIR; E43673; E43673.
DR   AlphaFoldDB; P27101; -.
DR   SMR; P27101; -.
DR   BioCyc; MetaCyc:MON-14415; -.
DR   UniPathway; UPA00083; -.
DR   GO; GO:0018506; F:maleylacetate reductase activity; NAS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0046300; P:2,4-dichlorophenoxyacetic acid catabolic process; NAS:UniProtKB.
DR   CDD; cd08177; MAR; 1.
DR   InterPro; IPR001670; ADH_Fe/GldA.
DR   InterPro; IPR039697; Alcohol_dehydrogenase_Fe.
DR   InterPro; IPR034786; MAR.
DR   PANTHER; PTHR11496; PTHR11496; 1.
DR   Pfam; PF00465; Fe-ADH; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; NAD; Oxidoreductase; Plasmid.
FT   CHAIN           1..352
FT                   /note="Maleylacetate reductase"
FT                   /id="PRO_0000087849"
SQ   SEQUENCE   352 AA;  37499 MW;  C72A9D2671FFFAB3 CRC64;
     MNFIHDPLTP RVLFGAGRLQ SLGEELKLLG IRRVLVISTP EQRELANQVA ALIPGSVAGF
     FDRATMHVPS QIVDQAASVA RELGVDSYVA PGGGSTIGLA KMLALHSSLP IVAIPTTYAG
     SEMTSIYGVT ENELKKTGRD RRVLARTVIY DPELTFGLPT GISVTSGLNA IAHAVEGLYA
     PEVNPILAIM AQQGIAALAK SIPTIRSAPT DLEARSQAQY GAWLCGSVLG NVSMALHHKL
     CHTLGGTFNL PHAETHTVVL PHALAYNTPA IPRANAWLQE ALATREPAQA LFDLAKSNGA
     PVSLQSIGMK EADLDRACEL VMSAQYPNPR PLEKHAIANL LRRAYLGEPP QP