ID   TCDA_BACSU              Reviewed;         254 AA.
AC   O32037;
DT   15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 3.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=tRNA threonylcarbamoyladenosine dehydratase;
DE            EC=6.1.-.-;
DE   AltName: Full=t(6)A37 dehydratase;
GN   Name=tcdA; Synonyms=yrvM; OrderedLocusNames=BSU27540;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [2]
RP   SEQUENCE REVISION TO 40.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
CC   -!- FUNCTION: Catalyzes the ATP-dependent dehydration of
CC       threonylcarbamoyladenosine at position 37 (t(6)A37) to form cyclic
CC       t(6)A37 (ct(6)A37) in tRNAs that read codons beginning with adenine.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the HesA/MoeB/ThiF family. {ECO:0000305}.
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DR   EMBL; AL009126; CAB14713.3; -; Genomic_DNA.
DR   RefSeq; NP_390632.3; NC_000964.3.
DR   RefSeq; WP_009967893.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; O32037; -.
DR   SMR; O32037; -.
DR   STRING; 224308.BSU27540; -.
DR   jPOST; O32037; -.
DR   PaxDb; O32037; -.
DR   PRIDE; O32037; -.
DR   EnsemblBacteria; CAB14713; CAB14713; BSU_27540.
DR   GeneID; 938590; -.
DR   KEGG; bsu:BSU27540; -.
DR   PATRIC; fig|224308.179.peg.2992; -.
DR   eggNOG; COG1179; Bacteria.
DR   InParanoid; O32037; -.
DR   OMA; DMDDICV; -.
DR   PhylomeDB; O32037; -.
DR   BioCyc; BSUB:BSU27540-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0061503; F:tRNA threonylcarbamoyladenosine dehydratase; IBA:GO_Central.
DR   GO; GO:0008641; F:ubiquitin-like modifier activating enzyme activity; IEA:InterPro.
DR   GO; GO:0061504; P:cyclic threonylcarbamoyladenosine biosynthetic process; IBA:GO_Central.
DR   InterPro; IPR045886; ThiF/MoeB/HesA.
DR   InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR   InterPro; IPR035985; Ubiquitin-activating_enz.
DR   PANTHER; PTHR43267; PTHR43267; 1.
DR   Pfam; PF00899; ThiF; 1.
DR   SUPFAM; SSF69572; SSF69572; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..254
FT                   /note="tRNA threonylcarbamoyladenosine dehydratase"
FT                   /id="PRO_0000389650"
SQ   SEQUENCE   254 AA;  28084 MW;  0AA9B6D50724635C CRC64;
     MLHQFSRNEL AIGKEGLETL KNSTVAVLGV GGVGSFAAEA LARSGVGRIL LVDKDDVDIT
     NVNRQLHALL STVGQPKVDL MKARIADINP ECEVIALKMF YTEETYEQFF DYGLDYVIDA
     SDTICYKIHL MKECLKRDIP LISSMGAANK TDPTRFQIAD ISKTHTDPIA KVVRTKLRKE
     GIKKGVQVIF SDESPIVIRE DVRKEVGNDE AKIRKAKMPP SSNAFVPSVA GLIMGGHVVM
     DLLKDIEIKR VKDK