TCDE_CLODI
ID TCDE_CLODI Reviewed; 166 AA.
AC P16153;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=Holin-like protein TcdE;
GN Name=tcdE {ECO:0000303|PubMed:22685398};
GN Synonyms=utxA {ECO:0000303|PubMed:1603068};
OS Clostridioides difficile (Peptoclostridium difficile).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC Clostridioides.
OX NCBI_TaxID=1496;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 4325 / VPI 10463;
RX PubMed=1603068; DOI=10.1007/bf00587587;
RA von Eichel-Streiber C., Laufenberg-Feldmann R., Sartingen S., Schulze J.,
RA Sauerborn M.;
RT "Comparative sequence analysis of the Clostridium difficile toxins A and
RT B.";
RL Mol. Gen. Genet. 233:260-268(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 4325 / VPI 10463;
RX PubMed=2105276; DOI=10.1128/iai.58.2.480-488.1990;
RA Dove C.H., Wang S.-Z., Price S.B., Phelps C.J., Lyerly D.M., Wilkins T.D.,
RA Johnson J.L.;
RT "Molecular characterization of the Clostridium difficile toxin A gene.";
RL Infect. Immun. 58:480-488(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 4325 / VPI 10463;
RA von Eichel-Streiber C.;
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 25-166.
RC STRAIN=ATCC 4325 / VPI 10463;
RX PubMed=2109310; DOI=10.1093/nar/18.6.1629;
RA Sauerborn M., von Eichel-Streiber C.;
RT "Nucleotide sequence of Clostridium difficile toxin A.";
RL Nucleic Acids Res. 18:1629-1630(1990).
RN [5]
RP FUNCTION, ALTERNATIVE INITIATION (ISOFORMS 1; 2 AND 3), SUBUNIT,
RP SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=22685398; DOI=10.1371/journal.ppat.1002727;
RA Govind R., Dupuy B.;
RT "Secretion of Clostridium difficile toxins A and B requires the holin-like
RT protein TcdE.";
RL PLoS Pathog. 8:e1002727-e1002727(2012).
CC -!- FUNCTION: [Isoform 1]: Holin-like protein required for secretion of
CC toxins A and B (TcdA and TcdB) (PubMed:22685398). Facilitates the
CC release of toxins to the extracellular environment without causing the
CC bacterial cell lysis (PubMed:22685398). {ECO:0000269|PubMed:22685398}.
CC -!- FUNCTION: [Isoform 2]: Has weak activity, suggesting that it may act as
CC a antiholin when multiple forms are produced.
CC {ECO:0000305|PubMed:22685398}.
CC -!- FUNCTION: [Isoform 3]: Has weak activity, suggesting that it may act as
CC a antiholin when multiple forms are produced.
CC {ECO:0000305|PubMed:22685398}.
CC -!- SUBUNIT: Homomultimer. {ECO:0000269|PubMed:22685398}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:22685398};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=3;
CC Name=1; Synonyms=Long {ECO:0000303|PubMed:1603068};
CC IsoId=P16153-1; Sequence=Displayed;
CC Name=2; Synonyms=Short {ECO:0000303|PubMed:1603068};
CC IsoId=P16153-2; Sequence=VSP_018824;
CC Name=3;
CC IsoId=P16153-3; Sequence=VSP_060769;
CC -!- DISRUPTION PHENOTYPE: Cells grow normally but accumulate a strongly
CC reduced amount of toxins TcdA and TcdB in the medium.
CC {ECO:0000269|PubMed:22685398}.
CC -!- SIMILARITY: Belongs to the bacteriophage holin family. {ECO:0000305}.
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DR EMBL; X60984; CAA43300.1; -; Genomic_DNA.
DR EMBL; X60984; CAA43301.1; -; Genomic_DNA.
DR EMBL; M30308; AAA83532.1; -; Genomic_DNA.
DR EMBL; M30308; AAA83533.1; -; Genomic_DNA.
DR EMBL; X92982; CAA63563.1; -; Genomic_DNA.
DR EMBL; X51797; CAA36093.1; -; Genomic_DNA.
DR PIR; B37052; B37052.
DR RefSeq; WP_003434540.1; NZ_VINH01000002.1. [P16153-1]
DR RefSeq; WP_018112585.1; NZ_BIWI01000430.1. [P16153-3]
DR AlphaFoldDB; P16153; -.
DR SMR; P16153; -.
DR TCDB; 1.E.19.3.1; the clostridium difficile tcde holin (tcde holin) family.
DR GeneID; 66353158; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0034291; F:canonical holin activity; IDA:CACAO.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:1901998; P:toxin transport; IMP:CACAO.
DR InterPro; IPR006480; Phage_holin_4_1.
DR Pfam; PF05105; Phage_holin_4_1; 1.
DR TIGRFAMs; TIGR01593; holin_tox_secr; 1.
PE 1: Evidence at protein level;
KW Alternative initiation; Cell membrane; Membrane; Protein transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..166
FT /note="Holin-like protein TcdE"
FT /id="PRO_0000022643"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..26
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_018824"
FT VAR_SEQ 1..24
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_060769"
SQ SEQUENCE 166 AA; 18800 MW; 1671FAE1884C7EE2 CRC64;
MHSSSPFYIS NGNKIFFYIN LGGVMNMTIS FLSEHIFIKL VILTISFDTL LGCLSAIKSR
KFNSSFGIDG GIRKVAMIAC IFFLSVVDIL TKFNFLFMLP QDCINFLRLK HLGISEFFSI
LFILYESVSI LKNMCLCGLP VPKRLKEKIA ILLDAMTDEM NAKDEK
