ID   TCD_SCHPO               Reviewed;         485 AA.
AC   O13861; Q9UT99;
DT   29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=tRNA threonylcarbamoyladenosine dehydratase;
DE            EC=6.1.-.-;
DE   AltName: Full=t(6)A37 dehydratase;
GN   ORFNames=SPAC1A6.10, SPAC30D11.05c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Catalyzes the ATP-dependent dehydration of
CC       threonylcarbamoyladenosine at position 37 (t(6)A37) to form cyclic
CC       t(6)A37 (ct(6)A37) in tRNAs that read codons beginning with adenine.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the HesA/MoeB/ThiF family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CU329670; CAB16360.1; -; Genomic_DNA.
DR   PIR; T38013; T38013.
DR   RefSeq; NP_593202.1; NM_001018598.2.
DR   AlphaFoldDB; O13861; -.
DR   SMR; O13861; -.
DR   STRING; 4896.SPAC1A6.10.1; -.
DR   iPTMnet; O13861; -.
DR   MaxQB; O13861; -.
DR   PaxDb; O13861; -.
DR   PRIDE; O13861; -.
DR   EnsemblFungi; SPAC1A6.10.1; SPAC1A6.10.1:pep; SPAC1A6.10.
DR   GeneID; 2542532; -.
DR   KEGG; spo:SPAC1A6.10; -.
DR   PomBase; SPAC1A6.10; -.
DR   VEuPathDB; FungiDB:SPAC1A6.10; -.
DR   eggNOG; KOG2018; Eukaryota.
DR   HOGENOM; CLU_013325_9_3_1; -.
DR   InParanoid; O13861; -.
DR   OMA; QISDISC; -.
DR   PhylomeDB; O13861; -.
DR   PRO; PR:O13861; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005741; C:mitochondrial outer membrane; ISO:PomBase.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0061503; F:tRNA threonylcarbamoyladenosine dehydratase; ISO:PomBase.
DR   GO; GO:0008641; F:ubiquitin-like modifier activating enzyme activity; ISM:PomBase.
DR   GO; GO:0061504; P:cyclic threonylcarbamoyladenosine biosynthetic process; ISO:PomBase.
DR   InterPro; IPR045886; ThiF/MoeB/HesA.
DR   InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR   InterPro; IPR035985; Ubiquitin-activating_enz.
DR   PANTHER; PTHR43267; PTHR43267; 1.
DR   Pfam; PF00899; ThiF; 1.
DR   SUPFAM; SSF69572; SSF69572; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Membrane; Mitochondrion; Mitochondrion outer membrane;
KW   Nucleotide-binding; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..485
FT                   /note="tRNA threonylcarbamoyladenosine dehydratase"
FT                   /id="PRO_0000120582"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        126..146
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        332..352
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   485 AA;  54299 MW;  2EBF9A725A44DD5F CRC64;
     METLQVEVFF LFDFTSIMAG FKVPSWITYK SFWIAVSSSV TTACVILGTL EFRKHRSIRR
     LQSMIVPEAG KSIQLSSSGV PIEIYDAGEE DEGISKGVPY DENLIREQLA RNYAFFGEDG
     MERLRNSFVI VVGCGGVGSW VINMLARSGV QKIRIVDFDQ VSLSSLNRHS IATLQDVGTP
     KTLAIKKAIK KFAPWIEVDA RNALFNPDSA DDLLSGNPDF VIDAIDNIQT KVDLLSYCYN
     HKLPVIASTG SACKSDPTRV NIADISATSE DPLSRATRRR LRLLGIMEGI PVVFSTEKPD
     PRKASLLPLS EEEFEKGDVD ELSALPEFRA RILPVIGPMP GIFGLTIATY VLTSIAKYPM
     DPISTMTRPR LYEEAVKRLH AEARKAGVNL DKTFNASEMS YIIEEVYVGR SALPPHESQK
     VTVVRWNPQL PFDHTNLVAM TRNEARYHED NVLAKNVDPS TVYGKDVIEV VHSFLRRLRM
     WEMLY