TCEA3_HUMAN
ID TCEA3_HUMAN Reviewed; 348 AA.
AC O75764; A8K2K7; Q5DR83;
DT 12-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 12-FEB-2003, sequence version 2.
DT 03-AUG-2022, entry version 182.
DE RecName: Full=Transcription elongation factor A protein 3;
DE AltName: Full=Transcription elongation factor S-II protein 3;
DE AltName: Full=Transcription elongation factor TFIIS.h;
GN Name=TCEA3; Synonyms=TFIISH;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC TISSUE=Embryonic rhabdomyosarcoma;
RA Behrends U., Gotz C., Mautner J.;
RT "SEREX-defined rhabdomyosarcoma antigens.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Colon;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 29-348 (ISOFORM 1).
RC TISSUE=Liver, and Spleen;
RX PubMed=9790746; DOI=10.1006/geno.1998.5449;
RA Labhart P., Morgan G.T.;
RT "Identification of novel genes encoding transcription elongation factor
RT TFIIS (TCEA) in vertebrates: conservation of three distinct TFIIS isoforms
RT in frog, mouse, and human.";
RL Genomics 52:278-288(1998).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115 AND SER-140, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: Necessary for efficient RNA polymerase II transcription
CC elongation past template-encoded arresting sites. The arresting sites
CC in DNA have the property of trapping a certain fraction of elongating
CC RNA polymerases that pass through, resulting in locked ternary
CC complexes. Cleavage of the nascent transcript by S-II allows the
CC resumption of elongation from the new 3'-terminus.
CC -!- INTERACTION:
CC O75764; Q8WVC0: LEO1; NbExp=3; IntAct=EBI-3913577, EBI-932432;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00649,
CC ECO:0000255|PROSITE-ProRule:PRU00651}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O75764-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O75764-2; Sequence=VSP_056838, VSP_056839;
CC -!- SIMILARITY: Belongs to the TFS-II family. {ECO:0000305}.
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DR EMBL; AY540752; AAT44402.1; -; mRNA.
DR EMBL; AK290272; BAF82961.1; -; mRNA.
DR EMBL; AL109936; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL357134; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC041613; AAH41613.1; -; mRNA.
DR EMBL; AJ223473; CAA11393.1; -; mRNA.
DR CCDS; CCDS44086.1; -. [O75764-1]
DR RefSeq; NP_003187.1; NM_003196.2. [O75764-1]
DR AlphaFoldDB; O75764; -.
DR SMR; O75764; -.
DR BioGRID; 112782; 6.
DR IntAct; O75764; 4.
DR STRING; 9606.ENSP00000406293; -.
DR iPTMnet; O75764; -.
DR PhosphoSitePlus; O75764; -.
DR BioMuta; TCEA3; -.
DR EPD; O75764; -.
DR jPOST; O75764; -.
DR MassIVE; O75764; -.
DR MaxQB; O75764; -.
DR PaxDb; O75764; -.
DR PeptideAtlas; O75764; -.
DR PRIDE; O75764; -.
DR ProteomicsDB; 50182; -. [O75764-1]
DR Antibodypedia; 8084; 203 antibodies from 24 providers.
DR DNASU; 6920; -.
DR Ensembl; ENST00000374601.7; ENSP00000363729.3; ENSG00000204219.11. [O75764-2]
DR Ensembl; ENST00000450454.7; ENSP00000406293.2; ENSG00000204219.11. [O75764-1]
DR Ensembl; ENST00000634832.1; ENSP00000488968.1; ENSG00000282941.3. [O75764-2]
DR Ensembl; ENST00000635035.2; ENSP00000489592.1; ENSG00000282941.3. [O75764-1]
DR GeneID; 6920; -.
DR KEGG; hsa:6920; -.
DR MANE-Select; ENST00000450454.7; ENSP00000406293.2; NM_003196.3; NP_003187.1.
DR UCSC; uc057dfl.1; human. [O75764-1]
DR CTD; 6920; -.
DR DisGeNET; 6920; -.
DR GeneCards; TCEA3; -.
DR HGNC; HGNC:11615; TCEA3.
DR HPA; ENSG00000204219; Tissue enhanced (skeletal).
DR MIM; 604128; gene.
DR neXtProt; NX_O75764; -.
DR OpenTargets; ENSG00000204219; -.
DR PharmGKB; PA36374; -.
DR VEuPathDB; HostDB:ENSG00000204219; -.
DR eggNOG; KOG1105; Eukaryota.
DR GeneTree; ENSGT00940000157034; -.
DR HOGENOM; CLU_037637_2_0_1; -.
DR InParanoid; O75764; -.
DR OMA; RKNMEGA; -.
DR OrthoDB; 1579101at2759; -.
DR PhylomeDB; O75764; -.
DR TreeFam; TF314970; -.
DR PathwayCommons; O75764; -.
DR SignaLink; O75764; -.
DR BioGRID-ORCS; 6920; 9 hits in 1074 CRISPR screens.
DR ChiTaRS; TCEA3; human.
DR GenomeRNAi; 6920; -.
DR Pharos; O75764; Tbio.
DR PRO; PR:O75764; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; O75764; protein.
DR Bgee; ENSG00000204219; Expressed in skeletal muscle tissue and 97 other tissues.
DR ExpressionAtlas; O75764; baseline and differential.
DR Genevisible; O75764; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.472.30; -; 1.
DR Gene3D; 1.20.930.10; -; 1.
DR InterPro; IPR035100; TF_IIS-typ.
DR InterPro; IPR003617; TFIIS/CRSP70_N_sub.
DR InterPro; IPR035441; TFIIS/LEDGF_dom_sf.
DR InterPro; IPR003618; TFIIS_cen_dom.
DR InterPro; IPR036575; TFIIS_cen_dom_sf.
DR InterPro; IPR017923; TFIIS_N.
DR InterPro; IPR006289; TFSII.
DR InterPro; IPR001222; Znf_TFIIS.
DR Pfam; PF08711; Med26; 1.
DR Pfam; PF01096; TFIIS_C; 1.
DR Pfam; PF07500; TFIIS_M; 1.
DR PIRSF; PIRSF006704; TF_IIS; 1.
DR SMART; SM00510; TFS2M; 1.
DR SMART; SM00509; TFS2N; 1.
DR SMART; SM00440; ZnF_C2C2; 1.
DR SUPFAM; SSF46942; SSF46942; 1.
DR SUPFAM; SSF47676; SSF47676; 1.
DR TIGRFAMs; TIGR01385; TFSII; 1.
DR PROSITE; PS51321; TFIIS_CENTRAL; 1.
DR PROSITE; PS51319; TFIIS_N; 1.
DR PROSITE; PS00466; ZF_TFIIS_1; 1.
DR PROSITE; PS51133; ZF_TFIIS_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..348
FT /note="Transcription elongation factor A protein 3"
FT /id="PRO_0000121452"
FT DOMAIN 5..82
FT /note="TFIIS N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00649"
FT DOMAIN 187..303
FT /note="TFIIS central"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00651"
FT ZN_FING 306..346
FT /note="TFIIS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00472"
FT REGION 78..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 82..132
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 133..147
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 310
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00472"
FT BINDING 313
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00472"
FT BINDING 338
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00472"
FT BINDING 341
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00472"
FT MOD_RES 115
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 140
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT VAR_SEQ 149..154
FT /note="SNSSKS -> ACSLMQ (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_056838"
FT VAR_SEQ 155..348
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_056839"
SQ SEQUENCE 348 AA; 38972 MW; 3D0A046B5096753D CRC64;
MGQEEELLRI AKKLEKMVAR KNTEGALDLL KKLHSCQMSI QLLQTTRIGV AVNGVRKHCS
DKEVVSLAKV LIKNWKRLLD SPGPPKGEKG EEREKAKKKE KGLECSDWKP EAGLSPPRKK
REDPKTRRDS VDSKSSASSS PKRPSVERSN SSKSKAESPK TPSSPLTPTF ASSMCLLAPC
YLTGDSVRDK CVEMLSAALK ADDDYKDYGV NCDKMASEIE DHIYQELKST DMKYRNRVRS
RISNLKDPRN PGLRRNVLSG AISAGLIAKM TAEEMASDEL RELRNAMTQE AIREHQMAKT
GGTTTDLFQC SKCKKKNCTY NQVQTRSADE PMTTFVLCNE CGNRWKFC
