TCF15_MESAU
ID TCF15_MESAU Reviewed; 195 AA.
AC Q60539;
DT 25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Transcription factor 15 {ECO:0000250|UniProtKB:Q60756};
DE Short=TCF-15 {ECO:0000250|UniProtKB:Q60756};
DE AltName: Full=Meso1 {ECO:0000303|PubMed:7597044};
DE AltName: Full=Paraxis {ECO:0000250|UniProtKB:Q60756};
DE AltName: Full=Protein bHLH-EC2 {ECO:0000250|UniProtKB:Q60756};
GN Name=TCF15 {ECO:0000250|UniProtKB:Q60756};
GN Synonyms=BHLHEC2 {ECO:0000250|UniProtKB:Q60756};
OS Mesocricetus auratus (Golden hamster).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC Cricetidae; Cricetinae; Mesocricetus.
OX NCBI_TaxID=10036;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7597044; DOI=10.1073/pnas.92.13.5870;
RA Blanar M.A., Crossley P.H., Peters K.G., Steingrimsson E., Copeland N.G.,
RA Jenkins N.A., Martin G.R., Rutter W.J.;
RT "Meso1, a basic-helix-loop-helix protein involved in mammalian presomitic
RT mesoderm development.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:5870-5874(1995).
CC -!- FUNCTION: Early transcription factor that plays a key role in
CC somitogenesis, paraxial mesoderm development and regulation of stem
CC cell pluripotency. Essential for the mesenchymal to epithelial
CC transition associated with somite formation. Required for somite
CC morphogenesis, thereby regulating patterning of the axial skeleton and
CC skeletal muscles. Required for proper localization of somite epithelium
CC markers during the mesenchymal to epithelial transition. Also plays a
CC key role in regulation of stem cell pluripotency. Promotes pluripotency
CC exit of embryonic stem cells (ESCs) by priming ESCs for
CC differentiation. Acts as a key regulator of self-renewal of
CC hematopoietic stem cells (HSCs) by mediating HSCs quiescence and long-
CC term self-renewal. Together with MEOX2, regulates transcription in
CC heart endothelial cells to regulate fatty acid transport across heart
CC endothelial cells. Acts by forming a heterodimer with another helix-
CC loop-helix (bHLH) protein, such as TCF3/E12, that binds DNA on E-box
CC motifs (5'-CANNTG-3') and activates transcription of target genes.
CC {ECO:0000250|UniProtKB:Q60756}.
CC -!- SUBUNIT: Heterodimer; efficient DNA binding requires dimerization with
CC another bHLH protein, such as TCF3/E12. Interacts with MEOX2.
CC {ECO:0000250|UniProtKB:Q60756}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q60756,
CC ECO:0000255|PROSITE-ProRule:PRU00981}.
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DR EMBL; L35922; AAA98996.1; -; mRNA.
DR RefSeq; NP_001269228.1; NM_001282299.1.
DR AlphaFoldDB; Q60539; -.
DR SMR; Q60539; -.
DR STRING; 10036.XP_005072579.1; -.
DR PRIDE; Q60539; -.
DR GeneID; 101826260; -.
DR CTD; 6939; -.
DR eggNOG; KOG4029; Eukaryota.
DR OrthoDB; 1511100at2759; -.
DR Proteomes; UP000189706; Unplaced.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IEA:Ensembl.
DR GO; GO:0043425; F:bHLH transcription factor binding; IEA:Ensembl.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:Ensembl.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR GO; GO:0070888; F:E-box binding; ISS:UniProtKB.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0043583; P:ear development; IEA:Ensembl.
DR GO; GO:0045198; P:establishment of epithelial cell apical/basal polarity; IEA:Ensembl.
DR GO; GO:0060231; P:mesenchymal to epithelial transition; IEA:Ensembl.
DR GO; GO:0048644; P:muscle organ morphogenesis; IEA:Ensembl.
DR GO; GO:1902037; P:negative regulation of hematopoietic stem cell differentiation; ISS:UniProtKB.
DR GO; GO:0050884; P:neuromuscular process controlling posture; IEA:Ensembl.
DR GO; GO:0048339; P:paraxial mesoderm development; IEA:Ensembl.
DR GO; GO:2000738; P:positive regulation of stem cell differentiation; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0036342; P:post-anal tail morphogenesis; IEA:Ensembl.
DR GO; GO:1903053; P:regulation of extracellular matrix organization; IEA:Ensembl.
DR GO; GO:0003016; P:respiratory system process; IEA:Ensembl.
DR GO; GO:0048705; P:skeletal system morphogenesis; IEA:Ensembl.
DR GO; GO:0001756; P:somitogenesis; IEA:Ensembl.
DR GO; GO:0019827; P:stem cell population maintenance; ISS:UniProtKB.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; Differentiation; DNA-binding; Nucleus;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..195
FT /note="Transcription factor 15"
FT /id="PRO_0000127461"
FT DOMAIN 70..122
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 26..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 60
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q60756"
SQ SEQUENCE 195 AA; 20732 MW; 1F70B4D2DF6276DB CRC64;
MAFALLRPVG AHVLYSDVRL LSEDEENRSE SDASDQSFGC CEGLETTRRG PGPGGGRRAS
SGAGPVVVVR QRQAANARER DRTQSVNTAF TALRTLIPTE PVDRKLSKIE TLRLASSYIA
HLANVLMLGD AADDGQPCFR AAGGGKSAVP AADGRQPRSI CTFCLSNQRK GGSRRDLGGS
CLKVRGVAPL RGPRR
