TCF21_MOUSE
ID TCF21_MOUSE Reviewed; 179 AA.
AC O35437; Q3U023;
DT 14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Transcription factor 21;
DE Short=TCF-21;
DE AltName: Full=Capsulin;
DE AltName: Full=Epicardin;
DE AltName: Full=Podocyte-expressed 1;
DE Short=Pod-1;
GN Name=Tcf21; Synonyms=Pod1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Embryo;
RX PubMed=9545526; DOI=10.1016/s0925-4773(98)00031-8;
RA Hidai H., Bardales R., Goodwin R., Quertermous T., Quertermous E.E.;
RT "Cloning of capsulin, a basic helix-loop-helix factor expressed in
RT progenitor cells of the pericardium and the coronary arteries.";
RL Mech. Dev. 73:33-43(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ; TISSUE=Embryonic heart, Fibroblast, and Uterus;
RX PubMed=9733105;
RX DOI=10.1002/(sici)1097-0177(199809)213:1<105::aid-aja10>3.0.co;2-1;
RA Robb L., Mifsud L., Hartley L., Biben C., Copeland N.G., Gilbert D.J.,
RA Jenkins N.A., Harvey R.P.;
RT "Epicardin: a novel basic helix-loop-helix transcription factor gene
RT expressed in epicardium, branchial arch myoblasts, and mesenchyme of
RT developing lung, gut, kidney, and gonads.";
RL Dev. Dyn. 213:105-113(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Embryo;
RX PubMed=9545521; DOI=10.1016/s0925-4773(98)00030-6;
RA Lu J.-R., Richardson J.A., Olson E.N.;
RT "Capsulin: a novel bHLH transcription factor expressed in epicardial
RT progenitors and mesenchyme of visceral organs.";
RL Mech. Dev. 73:23-32(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=129/Sv;
RX PubMed=10572052; DOI=10.1242/dev.126.24.5771;
RA Quaggin S.E., Schwartz L., Cui S., Igarashi P., Deimling J., Post M.,
RA Rossant J.;
RT "The basic-helix-loop-helix protein pod1 is critically important for kidney
RT and lung organogenesis.";
RL Development 126:5771-5783(1999).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11287187; DOI=10.1016/s0925-4773(01)00298-2;
RA Tamura M., Kanno Y., Chuma S., Saito T., Nakatsuji N.;
RT "Pod-1/Capsulin shows a sex- and stage-dependent expression pattern in the
RT mouse gonad development and represses expression of Ad4BP/SF-1.";
RL Mech. Dev. 102:135-144(2001).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Embryo, Muellerian duct, and Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP FUNCTION.
RX PubMed=10944221; DOI=10.1073/pnas.97.17.9525;
RA Lu J.-R., Chang P., Richardson J.A., Gan L., Weiler H., Olson E.N.;
RT "The basic helix-loop-helix transcription factor capsulin controls spleen
RT organogenesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:9525-9530(2000).
CC -!- FUNCTION: Involved in epithelial-mesenchymal interactions in kidney and
CC lung morphogenesis that include epithelial differentiation and
CC branching morphogenesis. May be involved in the organogenesis of the
CC spleen and heart and in cardiac and coronary artery development. May
CC function in the development and sex differentiation of gonad via
CC transcriptional regulation of AD4BP/SF-1.
CC {ECO:0000269|PubMed:10944221}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Forms a heterodimer with TCF3 and binds the E box (5'-CANNTG-
CC 3').
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed at high levels in lung, kidney, gut,
CC heart, ovary and podocytes (visceral glomerular epithelial cells). Also
CC found in spleen, large intestine, uterus, bladder and testis.
CC -!- DEVELOPMENTAL STAGE: Expressed during embryogenesis specifically in
CC mesodermally-derived cells that surround the epithelium of the
CC developing gastrointestinal, genitourinary, respiratory systems and in
CC spiral septum of the heart and in epicardial precursor cells fated to
CC form the coronary arteries. Expression pattern is sex- and stage-
CC dependent during gonadogenesis. At 13.5 dpc expressed at higher levels
CC in testis than ovary. In 3-month old adults expression drastically
CC decreased in testis while it increased in ovary showing an opposite
CC sex-dependent pattern in adults compared with fetuses. Expression in
CC other organs was similar between the adults and the fetal stage.
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DR EMBL; AF029753; AAB84256.1; -; mRNA.
DR EMBL; AF047418; AAC62531.1; -; mRNA.
DR EMBL; AF036945; AAC23537.1; -; mRNA.
DR EMBL; AF035717; AAC62513.1; -; mRNA.
DR EMBL; AB009453; BAA23883.1; -; mRNA.
DR EMBL; AK011575; BAB27709.1; -; mRNA.
DR EMBL; AK135423; BAE22527.1; -; mRNA.
DR EMBL; AK157289; BAE34032.1; -; mRNA.
DR EMBL; BC053525; AAH53525.1; -; mRNA.
DR CCDS; CCDS23732.1; -.
DR RefSeq; NP_035675.1; NM_011545.1.
DR AlphaFoldDB; O35437; -.
DR SMR; O35437; -.
DR BioGRID; 204005; 4.
DR IntAct; O35437; 3.
DR MINT; O35437; -.
DR STRING; 10090.ENSMUSP00000053178; -.
DR iPTMnet; O35437; -.
DR PhosphoSitePlus; O35437; -.
DR PaxDb; O35437; -.
DR PRIDE; O35437; -.
DR ProteomicsDB; 263089; -.
DR Antibodypedia; 19727; 161 antibodies from 23 providers.
DR DNASU; 21412; -.
DR Ensembl; ENSMUST00000049930; ENSMUSP00000053178; ENSMUSG00000045680.
DR GeneID; 21412; -.
DR KEGG; mmu:21412; -.
DR UCSC; uc007eps.2; mouse.
DR CTD; 6943; -.
DR MGI; MGI:1202715; Tcf21.
DR VEuPathDB; HostDB:ENSMUSG00000045680; -.
DR eggNOG; KOG4029; Eukaryota.
DR GeneTree; ENSGT00940000160174; -.
DR HOGENOM; CLU_092663_0_0_1; -.
DR InParanoid; O35437; -.
DR OrthoDB; 1374128at2759; -.
DR PhylomeDB; O35437; -.
DR TreeFam; TF350742; -.
DR BioGRID-ORCS; 21412; 0 hits in 70 CRISPR screens.
DR PRO; PR:O35437; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; O35437; protein.
DR Bgee; ENSMUSG00000045680; Expressed in left lung lobe and 176 other tissues.
DR ExpressionAtlas; O35437; baseline and differential.
DR Genevisible; O35437; MM.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0005667; C:transcription regulator complex; IDA:UniProtKB.
DR GO; GO:0043425; F:bHLH transcription factor binding; IPI:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:UniProtKB.
DR GO; GO:0070888; F:E-box binding; IDA:BHF-UCL.
DR GO; GO:0042826; F:histone deacetylase binding; IPI:BHF-UCL.
DR GO; GO:0050681; F:nuclear androgen receptor binding; IPI:BHF-UCL.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0009887; P:animal organ morphogenesis; IMP:MGI.
DR GO; GO:0001658; P:branching involved in ureteric bud morphogenesis; IMP:MGI.
DR GO; GO:0014707; P:branchiomeric skeletal muscle development; IGI:UniProtKB.
DR GO; GO:0060435; P:bronchiole development; IMP:MGI.
DR GO; GO:0032502; P:developmental process; IBA:GO_Central.
DR GO; GO:0060539; P:diaphragm development; IGI:UniProtKB.
DR GO; GO:0048557; P:embryonic digestive tract morphogenesis; ISS:UniProtKB.
DR GO; GO:0030855; P:epithelial cell differentiation; IMP:MGI.
DR GO; GO:0048732; P:gland development; ISS:UniProtKB.
DR GO; GO:0032835; P:glomerulus development; IMP:MGI.
DR GO; GO:0001822; P:kidney development; IMP:MGI.
DR GO; GO:0048286; P:lung alveolus development; IMP:MGI.
DR GO; GO:0060425; P:lung morphogenesis; IMP:MGI.
DR GO; GO:0060426; P:lung vasculature development; IMP:MGI.
DR GO; GO:0072277; P:metanephric glomerular capillary formation; IMP:UniProtKB.
DR GO; GO:0072162; P:metanephric mesenchymal cell differentiation; IMP:UniProtKB.
DR GO; GO:0001763; P:morphogenesis of a branching structure; IMP:MGI.
DR GO; GO:0060766; P:negative regulation of androgen receptor signaling pathway; IDA:BHF-UCL.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0031063; P:regulation of histone deacetylation; IC:BHF-UCL.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048608; P:reproductive structure development; IMP:MGI.
DR GO; GO:0060541; P:respiratory system development; IMP:MGI.
DR GO; GO:0060021; P:roof of mouth development; IGI:UniProtKB.
DR GO; GO:0060008; P:Sertoli cell differentiation; ISS:UniProtKB.
DR GO; GO:0007530; P:sex determination; IMP:MGI.
DR GO; GO:0007548; P:sex differentiation; IMP:MGI.
DR GO; GO:0048536; P:spleen development; IMP:UniProtKB.
DR GO; GO:0001657; P:ureteric bud development; IMP:MGI.
DR GO; GO:0001944; P:vasculature development; IMP:MGI.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..179
FT /note="Transcription factor 21"
FT /id="PRO_0000127465"
FT DOMAIN 79..131
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 19..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 27..49
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 67..81
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 179 AA; 19681 MW; 7BA3892818DB355F CRC64;
MSTGSLSDVE DLQEVEMLDC DSLKVDSNKE FGTSNESTEE GSNCENGSPQ KGRGGLGKRR
KAPTKKSPLS GVSQEGKQVQ RNAANARERA RMRVLSKAFS RLKTTLPWVP PDTKLSKLDT
LRLASSYIAH LRQILANDKY ENGYIHPVNL TWPFMVAGKP ENDLKEVVTA NRLCGTTAS
