TCF23_MOUSE
ID TCF23_MOUSE Reviewed; 209 AA.
AC Q9JLR5;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Transcription factor 23;
DE Short=TCF-23;
DE AltName: Full=Basic helix-loop-helix transcription factor OUT;
DE AltName: Full=Ovary, uterus and testis protein;
GN Name=Tcf23; Synonyms=Out;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, SUBUNIT, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=ICR; TISSUE=Ovary;
RX PubMed=10652346; DOI=10.1074/jbc.275.5.3510;
RA Narumi O., Mori S., Boku S., Tsuji Y., Hashimoto N., Nishikawa S.,
RA Yokota Y.;
RT "OUT, a novel basic helix-loop-helix transcription factor with an Id-like
RT inhibitory activity.";
RL J. Biol. Chem. 275:3510-3521(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Ovary, and Uterus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Inhibits E-box-mediated binding and transactivation of bHLH
CC factors. Inhibitory effect is similar to that of ID proteins. Inhibits
CC the formation of TCF3 and MYOD1 homodimers and heterodimers. Lacks DNA
CC binding activity. May be involved in the regulation or modulation of
CC smooth muscle contraction of the uterus during pregnancy and
CC particularly around the time of delivery. Seems to play a role in the
CC inhibition of myogenesis. {ECO:0000269|PubMed:10652346}.
CC -!- SUBUNIT: Forms inactive heterodimeric complex with TCF3.
CC {ECO:0000269|PubMed:10652346}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:10652346}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the uterus (predominantly in
CC myometrium), ovary, and testis. Expression in the uterus is higher in
CC the diestrus phase than in the estrus phase and reaches a maximum at
CC 7.5 dpc. Expression declines towards the time of delivery and returns
CC to the non-pregnant level 4 days after delivery. Low expression seen in
CC lung, heart, intestine, and spleen. {ECO:0000269|PubMed:10652346}.
CC -!- DOMAIN: Both the bHLH region and the C-terminal portion are essential
CC for inhibitory function.
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DR EMBL; AB059397; BAB69473.1; -; Genomic_DNA.
DR EMBL; AF142405; AAF31168.1; -; mRNA.
DR EMBL; AK030277; BAC26873.1; -; mRNA.
DR EMBL; AK030297; BAC26885.1; -; mRNA.
DR EMBL; BC119346; AAI19347.1; -; mRNA.
DR EMBL; BC120609; AAI20610.1; -; mRNA.
DR CCDS; CCDS19168.1; -.
DR RefSeq; NP_444315.1; NM_053085.2.
DR AlphaFoldDB; Q9JLR5; -.
DR SMR; Q9JLR5; -.
DR BioGRID; 213716; 1.
DR STRING; 10090.ENSMUSP00000006818; -.
DR iPTMnet; Q9JLR5; -.
DR PhosphoSitePlus; Q9JLR5; -.
DR PaxDb; Q9JLR5; -.
DR PRIDE; Q9JLR5; -.
DR ProteomicsDB; 263148; -.
DR Antibodypedia; 47343; 27 antibodies from 12 providers.
DR DNASU; 69852; -.
DR Ensembl; ENSMUST00000006818; ENSMUSP00000006818; ENSMUSG00000006642.
DR GeneID; 69852; -.
DR KEGG; mmu:69852; -.
DR UCSC; uc008wws.1; mouse.
DR CTD; 150921; -.
DR MGI; MGI:1934960; Tcf23.
DR VEuPathDB; HostDB:ENSMUSG00000006642; -.
DR eggNOG; KOG4029; Eukaryota.
DR GeneTree; ENSGT00940000161395; -.
DR HOGENOM; CLU_101416_1_0_1; -.
DR InParanoid; Q9JLR5; -.
DR OMA; RTRQDLW; -.
DR OrthoDB; 1462533at2759; -.
DR PhylomeDB; Q9JLR5; -.
DR TreeFam; TF350742; -.
DR BioGRID-ORCS; 69852; 5 hits in 72 CRISPR screens.
DR PRO; PR:Q9JLR5; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q9JLR5; protein.
DR Bgee; ENSMUSG00000006642; Expressed in pyloric antrum and 64 other tissues.
DR Genevisible; Q9JLR5; MM.
DR GO; GO:0000791; C:euchromatin; IDA:ARUK-UCL.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IPI:ARUK-UCL.
DR GO; GO:0140416; F:transcription regulator inhibitor activity; IDA:ARUK-UCL.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0046697; P:decidualization; ISO:MGI.
DR GO; GO:0032502; P:developmental process; IBA:GO_Central.
DR GO; GO:0007517; P:muscle organ development; IEA:UniProtKB-KW.
DR GO; GO:0051148; P:negative regulation of muscle cell differentiation; IDA:ARUK-UCL.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:ARUK-UCL.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; Myogenesis; Nucleus;
KW Reference proteome.
FT CHAIN 1..209
FT /note="Transcription factor 23"
FT /id="PRO_0000315821"
FT DOMAIN 75..127
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 54..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 209 AA; 22895 MW; 611E3878DE92446A CRC64;
MSQEATEAPA MPGEGHGHNK AKARWLLGTD RKRSRINRTR QDLWEDTSWS NHRLSRATSA
PRGTRARGTA HGRSEASPEN AARERTRVKT LRQAFLALQA ALPAVPPDTK LSKLDVLVLA
TSYIAHLTRT LGHELPGPAW PPFVRGLRYL HPLKKWPMRS RLYAGGLGCS DLDSTTAITT
GQRCKDAELG SQDSVAAESL LTSPAFGNK
