ID   TCMA_STRGA              Reviewed;         538 AA.
AC   P39886;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Tetracenomycin C resistance and export protein;
GN   Name=tcmA;
OS   Streptomyces glaucescens.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1907;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 40716 / ETH 22794 / Tue 49;
RX   PubMed=1592819; DOI=10.1128/jb.174.11.3651-3658.1992;
RA   Guilfoile P.G., Hutchinson C.R.;
RT   "Sequence and transcriptional analysis of the Streptomyces glaucescens
RT   tcmAR tetracenomycin C resistance and repressor gene loci.";
RL   J. Bacteriol. 174:3651-3658(1992).
CC   -!- FUNCTION: Resistance to tetracenomycin C by an active tetracenomycin C
CC       efflux system which is probably energized by transmembrane
CC       electrochemical gradients.
CC   -!- PATHWAY: Antibiotic biosynthesis; tetracenomycin C biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. EmrB family.
CC       {ECO:0000305}.
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DR   EMBL; M80674; AAA67509.1; -; Genomic_DNA.
DR   PIR; A41901; A41901.
DR   RefSeq; WP_052413892.1; NZ_CP009438.1.
DR   AlphaFoldDB; P39886; -.
DR   SMR; P39886; -.
DR   STRING; 1907.SGLAU_26325; -.
DR   TCDB; 2.A.1.3.12; the major facilitator superfamily (mfs).
DR   eggNOG; COG0477; Bacteria.
DR   OMA; WDSAYFI; -.
DR   OrthoDB; 1108236at2; -.
DR   UniPathway; UPA00174; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR004638; EmrB-like.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   TIGRFAMs; TIGR00711; efflux_EmrB; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Antibiotic resistance; Cell membrane; Membrane;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..538
FT                   /note="Tetracenomycin C resistance and export protein"
FT                   /id="PRO_0000173366"
FT   TRANSMEM        28..48
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        126..146
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        154..174
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        181..201
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        213..233
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        239..259
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        286..306
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        319..339
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        342..362
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        371..391
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        413..433
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        494..514
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   538 AA;  54846 MW;  DED3F28C1F22AA56 CRC64;
     MSTETHDEPS GVAHTPASGL RGRPWPTLLA VAVGVMMVAL DSTIVAIANP AIQQDLHASL
     ADVQWITNGY LLALAVSLIT AGKLGDRFGH RQTFLVGVAG FAVTSAAIGL SGSVAAIVVF
     RVLQGLFGAL MQPSALGLLR VTFPPGKLNM AIGIWSGVVG ASTAAGPIIG GLLVQHVGWE
     AVFFINVPVG LAALVAGLVI LTDARAERAP KSFDVSGIVL LSGAMFCLVW GLIKAPAWGW
     GDLRTLGFLA AAVLAFAGFT LRESRATEPL MPLAMFRSVP LSAGTVLMVL MAFSFIGGLF
     FVTFYLQNVH GMSPVESGVH LLPLTGMMIV GAPVSGIVIS RFGPGGPLVV GMLLTAASLW
     GMSTLEADSG MGITSLWFVL LGLGLAPVMV GTTDVIVSNA PAELAGVAGG LQQSAMQVGG
     SLGTAVLGVL MASRVGDVFP DKWAEANLPR VGPREAAAIE DAAEVGAVPP AGTLPGRHAG
     TLSEVVHSSF ISGMGLAFTV AGAVALVAAA VALFTRKAEP DERAPEEFPV PASTAGRG