ID   TCMG_STRGA              Reviewed;         572 AA.
AC   P39888;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Tetracenomycin polyketide synthesis hydroxylase TcmG;
DE            EC=1.14.13.-;
GN   Name=tcmG;
OS   Streptomyces glaucescens.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1907;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 40716 / ETH 22794 / Tue 49;
RX   PubMed=8509339; DOI=10.1128/jb.175.12.3876-3886.1993;
RA   Decker H., Motamedi H., Hutchinson C.R.;
RT   "Nucleotide sequences and heterologous expression of tcmG and tcmP,
RT   biosynthetic genes for tetracenomycin C in Streptomyces glaucescens.";
RL   J. Bacteriol. 175:3876-3886(1993).
CC   -!- FUNCTION: Hydroxylates tetracenomycin A2 at positions C-4, C-4A, and
CC       C12A to give tetracenomycin C (TCM C). Hydroxylates as well 9-
CC       carboxymethyl-TCM B3 to yield 8-demethyl-TMC C, a side reaction.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- PATHWAY: Antibiotic biosynthesis; tetracenomycin C biosynthesis.
CC   -!- SIMILARITY: Belongs to the PheA/TfdB FAD monooxygenase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA67511.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; M80674; AAA67511.1; ALT_INIT; Genomic_DNA.
DR   PIR; A47127; A47127.
DR   AlphaFoldDB; P39888; -.
DR   SMR; P39888; -.
DR   STRING; 1907.SGLAU_26335; -.
DR   eggNOG; COG0654; Bacteria.
DR   BioCyc; MetaCyc:MON-18607; -.
DR   BRENDA; 1.14.13.200; 6020.
DR   UniPathway; UPA00174; -.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002938; FAD-bd.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   Pfam; PF01494; FAD_binding_3; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; FAD; Flavoprotein; Oxidoreductase.
FT   CHAIN           1..572
FT                   /note="Tetracenomycin polyketide synthesis hydroxylase
FT                   TcmG"
FT                   /id="PRO_0000214048"
FT   BINDING         18..47
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
FT   BINDING         323..333
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   572 AA;  61727 MW;  57AADC23ECE79269 CRC64;
     MPVSDRPKGC ILSTEEVPVL IVGGGLTGLS AALFLSQHGV SCRLVEKHRG TTVLTRASGI
     SSRTMELLRG VGLERTVIER GPKLVEGARW RELGQPADQI PWVVIRANGL HDLENAVTVE
     EPSLDVGHLS PTRPYWCGQD RLEPILRDEA VRRGARIDFN TRMEAFTADE SGVTATIVDQ
     ATGEQSTVRA RYLIAADGVR SPVRETLGIT RTGHGTIGNA MSVLFKADLR DTVKGRRFVI
     CYLPNPDEPG VLQLPEVPAV LQLFDEDRWI FGFFFDPRET SPEQFTDERC AQIIRTATGL
     PGLPVEVQMA RPWEMSHNSA RSYRSGRVFL AGDAAHVHPP AGAFGANGGI QDAHNLAWKL
     AAVLKGTAGD ALLDTYEQER LPIGAAVADQ AWIRHTWRLN DSEELRRALR ESTLVATGYR
     YTSDAVLGAA YPEPIPAAHD LTGRPGYRVP HVWLGRGGER VSTVDLGGDA FVVLAGPDGG
     EWQAAADKVA QDLGVPVHCH PVGGDGQLTD PDGAFLGTTG LTRNGALLIR PDGFVAWRAE
     YLPEDAAGEL RSALERILAR TSGTPGGTAL EG