ID   TCMH_STRGA              Reviewed;         112 AA.
AC   P39889;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Tetracenomycin-F1 monooxygenase;
DE            EC=1.13.12.21;
DE   AltName: Full=Tetracenomycin polyketide synthesis hydroxylase TcmH;
GN   Name=tcmH;
OS   Streptomyces glaucescens.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1907;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 40716 / ETH 22794 / Tue 49;
RX   PubMed=8244926; DOI=10.1128/jb.175.23.7571-7580.1993;
RA   Summers R.G., Wendt-Pienkowski E., Motamedi H., Hutchinson C.R.;
RT   "The tcmVI region of the tetracenomycin C biosynthetic gene cluster of
RT   Streptomyces glaucescens encodes the tetracenomycin F1 monooxygenase,
RT   tetracenomycin F2 cyclase, and, most likely, a second cyclase.";
RL   J. Bacteriol. 175:7571-7580(1993).
RN   [2]
RP   PROTEIN SEQUENCE OF 2-19, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, ACTIVITY
RP   REGULATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=8329392; DOI=10.1021/bi00077a019;
RA   Shen B., Hutchinson C.R.;
RT   "Tetracenomycin F1 monooxygenase: oxidation of a naphthacenone to a
RT   naphthacenequinone in the biosynthesis of tetracenomycin C in Streptomyces
RT   glaucescens.";
RL   Biochemistry 32:6656-6663(1993).
CC   -!- FUNCTION: Oxygenase required for conversion of tetracenomycin F1 to
CC       tetracenomycin D3. {ECO:0000269|PubMed:8329392}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=O2 + tetracenomycin F1 = H(+) + H2O + tetracenomycin D3;
CC         Xref=Rhea:RHEA:37447, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:74931, ChEBI:CHEBI:77990;
CC         EC=1.13.12.21; Evidence={ECO:0000269|PubMed:8329392};
CC   -!- ACTIVITY REGULATION: Inhibited by p-chloromercuribenzoic acid, N-
CC       ethylmaleimide and diethyl pyrocarbonate. {ECO:0000269|PubMed:8329392}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=7.47 uM for tetracenomycin F1 {ECO:0000269|PubMed:8329392};
CC         Vmax=473 nmol/min/mg enzyme {ECO:0000269|PubMed:8329392};
CC       pH dependence:
CC         Optimum pH is 7.5. {ECO:0000269|PubMed:8329392};
CC   -!- PATHWAY: Antibiotic biosynthesis; tetracenomycin C biosynthesis.
CC   -!- SUBUNIT: Homotrimer. {ECO:0000269|PubMed:8329392}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M80674; AAA67512.1; -; Genomic_DNA.
DR   PIR; A53291; A53291.
DR   RefSeq; WP_052413894.1; NZ_CP009438.1.
DR   AlphaFoldDB; P39889; -.
DR   SMR; P39889; -.
DR   STRING; 1907.SGLAU_26340; -.
DR   eggNOG; COG2329; Bacteria.
DR   OrthoDB; 1909149at2; -.
DR   BioCyc; MetaCyc:MON-18103; -.
DR   UniPathway; UPA00174; -.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR007138; ABM_dom.
DR   InterPro; IPR011008; Dimeric_a/b-barrel.
DR   Pfam; PF03992; ABM; 1.
DR   SUPFAM; SSF54909; SSF54909; 1.
DR   PROSITE; PS51725; ABM; 1.
PE   1: Evidence at protein level;
KW   Antibiotic biosynthesis; Direct protein sequencing; Oxidoreductase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8329392"
FT   CHAIN           2..112
FT                   /note="Tetracenomycin-F1 monooxygenase"
FT                   /id="PRO_0000072453"
FT   DOMAIN          11..100
FT                   /note="ABM"
SQ   SEQUENCE   112 AA;  12683 MW;  242ACFF596F73C9F CRC64;
     MATISPSPDL FTLVNVFGVA PEKQRELRDH LVQVTEDLIR HMPGFVSATF HLSRDGEQVV
     NYAQWRSEAD FRAMHADPRL QPHFDYCRSV SRPKPIFCEV THSFGATSPE GA