TCMO_ORYSJ
ID TCMO_ORYSJ Reviewed; 500 AA.
AC Q5W6F1; A0A0N7KKJ1;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Trans-cinnamate 4-monooxygenase {ECO:0000305};
DE EC=1.14.14.91 {ECO:0000250|UniProtKB:Q94IP1};
DE AltName: Full=Cinnamic acid 4-hydroxylase {ECO:0000303|Ref.1};
DE Short=C4H {ECO:0000305};
DE Short=CA4H {ECO:0000305};
DE AltName: Full=Cinnamic acid 4-hydroxylase-like protein {ECO:0000303|Ref.1};
DE Short=C4H-like {ECO:0000303|Ref.1};
DE Short=OsC4HL {ECO:0000303|Ref.1};
DE AltName: Full=Cytochrome P450 73A33 {ECO:0000305};
DE AltName: Full=Cytochrome P450C4H {ECO:0000305};
GN Name=C4HL {ECO:0000303|Ref.1};
GN OrderedLocusNames=Os05g0320700 {ECO:0000312|EMBL:BAS93361.1},
GN LOC_Os05g25640 {ECO:0000305};
GN ORFNames=OsJ_18060 {ECO:0000312|EMBL:EEE63250.1},
GN OSJNBb0006B22.11 {ECO:0000312|EMBL:AAV44089.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION BY WOUNDING.
RX DOI=10.1007/BF03030528;
RA Yang D.H., Chung B.Y., Kim J.S., Kim J.H., Yun P.Y., Lee Y.K., Lim Y.P.,
RA Lee M.C.;
RT "cDNA cloning and sequence analysis of the rice cinnamate-4-hydroxylase
RT gene, a cytochrome p450-dependent monooxygenase involved in the general
RT phenylpropanoid pathway.";
RL J. Plant Biol. 48:311-318(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Catalyzes the first oxidative step of the phenylpropanoid
CC pathway in higher plants by transforming trans-cinnamate into p-
CC coumarate (By similarity). The compounds formed by this pathway are
CC essential components for lignification, pollination, and defense
CC against ultraviolet light, predators and pathogens (By similarity).
CC {ECO:0000250|UniProtKB:Q94IP1}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-cinnamate + O2 + reduced [NADPH--hemoprotein reductase] =
CC (E)-4-coumarate + H(+) + H2O + oxidized [NADPH--hemoprotein
CC reductase]; Xref=Rhea:RHEA:10608, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC COMP:11965, ChEBI:CHEBI:12876, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:15669, ChEBI:CHEBI:57618,
CC ChEBI:CHEBI:58210; EC=1.14.14.91;
CC Evidence={ECO:0000250|UniProtKB:Q94IP1};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:10609;
CC Evidence={ECO:0000250|UniProtKB:Q94IP1};
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:Q94IP1};
CC -!- PATHWAY: Phenylpropanoid metabolism; trans-4-coumarate biosynthesis;
CC trans-4-coumarate from trans-cinnamate: step 1/1. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and leaves. {ECO:0000269|Ref.1}.
CC -!- INDUCTION: Induced by wounding. {ECO:0000269|Ref.1}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB207105; BAF45113.1; -; mRNA.
DR EMBL; AC136224; AAV44089.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF17104.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS93361.1; -; Genomic_DNA.
DR EMBL; CM000142; EEE63250.1; -; Genomic_DNA.
DR EMBL; AK100598; BAG94673.1; -; mRNA.
DR EMBL; AK104994; BAG97065.1; -; mRNA.
DR RefSeq; XP_015639656.1; XM_015784170.1.
DR AlphaFoldDB; Q5W6F1; -.
DR SMR; Q5W6F1; -.
DR STRING; 4530.OS05T0320700-02; -.
DR PaxDb; Q5W6F1; -.
DR PRIDE; Q5W6F1; -.
DR EnsemblPlants; Os05t0320700-02; Os05t0320700-02; Os05g0320700.
DR GeneID; 4338409; -.
DR Gramene; Os05t0320700-02; Os05t0320700-02; Os05g0320700.
DR KEGG; osa:4338409; -.
DR eggNOG; KOG0156; Eukaryota.
DR HOGENOM; CLU_001570_4_0_1; -.
DR InParanoid; Q5W6F1; -.
DR OMA; IHRHPRD; -.
DR OrthoDB; 702827at2759; -.
DR PlantReactome; R-OSA-1119418; Suberin biosynthesis.
DR PlantReactome; R-OSA-1119582; Phenylpropanoid biosynthesis, initial reactions.
DR UniPathway; UPA00825; UER00789.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000007752; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016710; F:trans-cinnamate 4-monooxygenase activity; IBA:GO_Central.
DR GO; GO:0009808; P:lignin metabolic process; IBA:GO_Central.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..500
FT /note="Trans-cinnamate 4-monooxygenase"
FT /id="PRO_0000451426"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 213..218
FT /ligand="(E)-cinnamate"
FT /ligand_id="ChEBI:CHEBI:15669"
FT /evidence="ECO:0000250|UniProtKB:Q94IP1"
FT BINDING 302
FT /ligand="(E)-cinnamate"
FT /ligand_id="ChEBI:CHEBI:15669"
FT /evidence="ECO:0000250|UniProtKB:Q94IP1"
FT BINDING 442
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:Q94IP1"
SQ SEQUENCE 500 AA; 56883 MW; FD4242B9BE859870 CRC64;
MDALLVEKVL LGLFVAAVLA LVVAKLTGKR LRLPPGPAGA PIVGNWLQVG DDLNHRNLMA
LARRFGDILL LRMGVRNLVV VSSPDLAKEV LHTQGVEFGS RTRNVVFDIF TGKGQDMVFT
VYGDHWRKMR RIMTVPFFTN KVVAQNRAGW EEEARLVVED VRRDPAAATS GVVIRRRLQL
MMYNDMFRIM FDRRFDSVDD PLFNKLKAFN AERSRLSQSF EYNYGDFIPV LRPFLRRYLA
RCHQLKSQRM KLFEDHFVQE RKRVMEQTGE IRCAMDHILE AERKGEINHD NVLYIVENIN
VAAIETTLWS IEWGIAELVN HPSIQSKVRE EMASVLGGAA VTEPDLERLP YLQAVVKETL
RLRMAIPLLV PHMNLADGKL AGYDIPAESK ILVNAWFLAN DPKRWVRPDE FRPERFLEEE
KAVEAHGNDF RFVPFGVGRR SCPGIILALP IIGITLGRLV QSFDLLPPPG MDKVDTTEKP
GQFSNQILKH ATVVCKPIDA
