ID   TCMO_STRGA              Reviewed;         339 AA.
AC   P39896;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Tetracenomycin polyketide synthesis 8-O-methyl transferase TcmO;
DE            EC=2.1.1.-;
GN   Name=tcmO;
OS   Streptomyces glaucescens.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1907;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 40716 / ETH 22794 / Tue 49;
RX   PubMed=1548230; DOI=10.1128/jb.174.6.1810-1820.1992;
RA   Summers R.G., Wendt-Pienkowski E., Motamedi H., Hutchinson C.R.;
RT   "Nucleotide sequence of the tcmII-tcmIV region of the tetracenomycin C
RT   biosynthetic gene cluster of Streptomyces glaucescens and evidence that the
RT   tcmN gene encodes a multifunctional cyclase-dehydratase-O-methyl
RT   transferase.";
RL   J. Bacteriol. 174:1810-1820(1992).
CC   -!- PATHWAY: Antibiotic biosynthesis; tetracenomycin C biosynthesis.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. Cation-independent O-methyltransferase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU01020}.
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DR   EMBL; M80674; AAA67519.1; -; Genomic_DNA.
DR   PIR; C42276; C42276.
DR   RefSeq; WP_043504920.1; NZ_CP009438.1.
DR   AlphaFoldDB; P39896; -.
DR   SMR; P39896; -.
DR   STRING; 1907.SGLAU_26375; -.
DR   eggNOG; COG2345; Bacteria.
DR   OMA; NTNHGEL; -.
DR   OrthoDB; 1213908at2; -.
DR   BioCyc; MetaCyc:MON-18605; -.
DR   UniPathway; UPA00174; -.
DR   GO; GO:0008171; F:O-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR031725; ASMT_dimerisation.
DR   InterPro; IPR016461; COMT-like.
DR   InterPro; IPR001077; O_MeTrfase_dom.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR11746; PTHR11746; 1.
DR   Pfam; PF16864; Dimerisation2; 1.
DR   Pfam; PF00891; Methyltransf_2; 1.
DR   PIRSF; PIRSF005739; O-mtase; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51683; SAM_OMT_II; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..339
FT                   /note="Tetracenomycin polyketide synthesis 8-O-methyl
FT                   transferase TcmO"
FT                   /id="PRO_0000072457"
FT   ACT_SITE        246
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01020"
FT   BINDING         200
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01020"
FT   BINDING         226..228
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01020"
SQ   SEQUENCE   339 AA;  37035 MW;  B228B66B24217F80 CRC64;
     MTPHTHVRGP GDILQLTMAF YGSRALISAV ELDLFTLLAG KPLPLGELCE RAGIHPRGAR
     DFLDALVALG LLEREGEDTY RNSPAADRHL DRRKPGYVGG YARLADTKLF PVWARLTEAL
     RTGEKQVPSQ GGFFGGYADP EAARGFLGAM DAVNGGVGHS LAGALDWTEY SSFVDLGGAR
     GNLAAHLHRA HPHLRATCFD LPEMEPFFQE HMKSLETTDQ VRFAGGDFFT DPLPRADVFI
     VGHILHYFGL RQREALIARI HQALTPGGAV LVYDRMIDDD RRSAALSLLG SLNMLLTSDE
     GREYTPAECV RWLSDAGFTD VRTTAVSGPD TLAIGRKPR