TCP1L_HUMAN
ID TCP1L_HUMAN Reviewed; 215 AA.
AC Q8TDR4; Q53EW0; Q96LN5;
DT 10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=T-complex protein 10A homolog 1;
DE Short=T-complex protein 10A-1;
DE Short=TCP10A-1;
DE AltName: Full=TCP10-like;
GN Name=TCP10L; ORFNames=PRED77;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND MUTAGENESIS OF LEU-82.
RX PubMed=14586771; DOI=10.1007/s10038-003-0075-6;
RA Chen Z., Yu L., Wu H., Yu J., Zhang L., Jiang D., Ma L., Li D., Zhao S.;
RT "Identification of a novel liver-specific expressed gene, TCP10L, encoding
RT a human leucine zipper protein with transcription inhibition activity.";
RL J. Hum. Genet. 48:556-563(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ARG-145.
RC TISSUE=Testis;
RA Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT ARG-145.
RX PubMed=10830953; DOI=10.1038/35012518;
RA Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S.,
RA Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M.,
RA Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A., Menzel U.,
RA Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A.,
RA Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J.,
RA Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K.,
RA Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G.,
RA Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J.,
RA Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S.,
RA Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K.,
RA Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.;
RT "The DNA sequence of human chromosome 21.";
RL Nature 405:311-319(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH ZIPK/DAPK3.
RX PubMed=15910542; DOI=10.1111/j.1365-2605.2005.00522.x;
RA Yu H., Jiang D., Guo Z., Saiyin H., Guo J., Wang X., Yu L.;
RT "TCP10L is expressed specifically in spermatogenic cells and binds to death
RT associated protein kinase-3.";
RL Int. J. Androl. 28:163-170(2005).
RN [7]
RP INTERACTION WITH MAD4.
RX PubMed=15469726; DOI=10.5483/bmbrep.2004.37.4.402;
RA Jiang D.J., Yu H.X., Hexige S.Y., Guo Z.K., Wang X., Ma L.J., Chen Z.,
RA Zhao S.Y., Yu L.;
RT "Human liver specific transcriptional factor TCP10L binds to MAD4.";
RL J. Biochem. Mol. Biol. 37:402-407(2004).
RN [8]
RP SELF-ASSOCIATION, AND MUTAGENESIS OF LEU-89.
RX PubMed=17377852; DOI=10.1007/s11033-007-9067-5;
RA Zhong Z., Qiu J., Chen X., Wan B., Ni J., Yang Y., Bai M., Zhang H., Yu L.;
RT "Identification of TCP10L as primate-specific gene derived via segmental
RT duplication and homodimerization of TCP10L through the leucine zipper
RT motif.";
RL Mol. Biol. Rep. 35:171-178(2008).
RN [9]
RP FUNCTION.
RX PubMed=24565846; DOI=10.1016/j.bbrc.2014.02.049;
RA Zuo J., Cai H., Wu Y., Ma H., Jiang W., Liu C., Han D., Ji G., Yu L.;
RT "TCP10L acts as a tumor suppressor by inhibiting cell proliferation in
RT hepatocellular carcinoma.";
RL Biochem. Biophys. Res. Commun. 446:61-67(2014).
CC -!- FUNCTION: May be involved in transcriptional regulation. Has in vitro
CC transcription inhibition activity. Acts as a tumor suppressor in
CC hepatocellular carcinoma (HCC) cells. {ECO:0000269|PubMed:14586771,
CC ECO:0000269|PubMed:24565846}.
CC -!- SUBUNIT: Self-associates (via leucine zipper). Interacts (via leucine
CC zipper) with ZIPK/DAPK3 (via leucine zipper). Interacts with MAD4.
CC {ECO:0000269|PubMed:15469726, ECO:0000269|PubMed:15910542,
CC ECO:0000269|PubMed:17377852}.
CC -!- INTERACTION:
CC Q8TDR4; Q9Y5Z0: BACE2; NbExp=3; IntAct=EBI-3923210, EBI-11282723;
CC Q8TDR4; Q53TS8: C2CD6; NbExp=3; IntAct=EBI-3923210, EBI-739879;
CC Q8TDR4; Q68D86: CCDC102B; NbExp=3; IntAct=EBI-3923210, EBI-10171570;
CC Q8TDR4; Q2TAC2-2: CCDC57; NbExp=3; IntAct=EBI-3923210, EBI-10961624;
CC Q8TDR4; Q6NSX1: CCDC70; NbExp=3; IntAct=EBI-3923210, EBI-6873045;
CC Q8TDR4; Q86X02: CDR2L; NbExp=3; IntAct=EBI-3923210, EBI-11063830;
CC Q8TDR4; O76071: CIAO1; NbExp=3; IntAct=EBI-3923210, EBI-725145;
CC Q8TDR4; P02489: CRYAA; NbExp=3; IntAct=EBI-3923210, EBI-6875961;
CC Q8TDR4; O43293: DAPK3; NbExp=5; IntAct=EBI-3923210, EBI-77293;
CC Q8TDR4; Q14192: FHL2; NbExp=3; IntAct=EBI-3923210, EBI-701903;
CC Q8TDR4; O43559: FRS3; NbExp=3; IntAct=EBI-3923210, EBI-725515;
CC Q8TDR4; Q4V328: GRIPAP1; NbExp=3; IntAct=EBI-3923210, EBI-717919;
CC Q8TDR4; P28799-2: GRN; NbExp=3; IntAct=EBI-3923210, EBI-25860013;
CC Q8TDR4; P42858: HTT; NbExp=3; IntAct=EBI-3923210, EBI-466029;
CC Q8TDR4; Q92876: KLK6; NbExp=3; IntAct=EBI-3923210, EBI-2432309;
CC Q8TDR4; P25800: LMO1; NbExp=3; IntAct=EBI-3923210, EBI-8639312;
CC Q8TDR4; P25791-3: LMO2; NbExp=3; IntAct=EBI-3923210, EBI-11959475;
CC Q8TDR4; Q8TAP4-4: LMO3; NbExp=3; IntAct=EBI-3923210, EBI-11742507;
CC Q8TDR4; P45984: MAPK9; NbExp=3; IntAct=EBI-3923210, EBI-713568;
CC Q8TDR4; P55081: MFAP1; NbExp=3; IntAct=EBI-3923210, EBI-1048159;
CC Q8TDR4; Q14582: MXD4; NbExp=6; IntAct=EBI-3923210, EBI-3943670;
CC Q8TDR4; O43639: NCK2; NbExp=3; IntAct=EBI-3923210, EBI-713635;
CC Q8TDR4; I6L9F6: NEFL; NbExp=3; IntAct=EBI-3923210, EBI-10178578;
CC Q8TDR4; P07196: NEFL; NbExp=3; IntAct=EBI-3923210, EBI-475646;
CC Q8TDR4; Q96HA8: NTAQ1; NbExp=3; IntAct=EBI-3923210, EBI-741158;
CC Q8TDR4; Q9UBU9: NXF1; NbExp=3; IntAct=EBI-3923210, EBI-398874;
CC Q8TDR4; Q13153: PAK1; NbExp=3; IntAct=EBI-3923210, EBI-1307;
CC Q8TDR4; P05129: PRKCG; NbExp=3; IntAct=EBI-3923210, EBI-949799;
CC Q8TDR4; P41219: PRPH; NbExp=6; IntAct=EBI-3923210, EBI-752074;
CC Q8TDR4; Q15311: RALBP1; NbExp=3; IntAct=EBI-3923210, EBI-749285;
CC Q8TDR4; Q96GM5: SMARCD1; NbExp=3; IntAct=EBI-3923210, EBI-358489;
CC Q8TDR4; Q8N8B7-2: TCEANC; NbExp=3; IntAct=EBI-3923210, EBI-11955057;
CC Q8TDR4; Q8TDR4: TCP10L; NbExp=2; IntAct=EBI-3923210, EBI-3923210;
CC Q8TDR4; Q96PN8: TSSK3; NbExp=3; IntAct=EBI-3923210, EBI-3918381;
CC Q8TDR4; O76024: WFS1; NbExp=3; IntAct=EBI-3923210, EBI-720609;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14586771,
CC ECO:0000269|PubMed:15910542}.
CC -!- TISSUE SPECIFICITY: Expressed in liver and testis. Expressed in the
CC seminiferous tubules (at protein level). {ECO:0000269|PubMed:14586771,
CC ECO:0000269|PubMed:15910542}.
CC -!- SIMILARITY: Belongs to the TCP10 family. {ECO:0000305}.
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DR EMBL; AF115967; AAM18531.1; -; mRNA.
DR EMBL; AK058078; BAB71653.1; -; mRNA.
DR EMBL; AK223529; BAD97249.1; -; mRNA.
DR EMBL; AP001714; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC033855; AAH33855.1; -; mRNA.
DR CCDS; CCDS13616.1; -.
DR RefSeq; NP_653260.1; NM_144659.5.
DR AlphaFoldDB; Q8TDR4; -.
DR SMR; Q8TDR4; -.
DR BioGRID; 126602; 71.
DR IntAct; Q8TDR4; 72.
DR STRING; 9606.ENSP00000300258; -.
DR iPTMnet; Q8TDR4; -.
DR PhosphoSitePlus; Q8TDR4; -.
DR BioMuta; TCP10L; -.
DR DMDM; 23822246; -.
DR MassIVE; Q8TDR4; -.
DR PaxDb; Q8TDR4; -.
DR PeptideAtlas; Q8TDR4; -.
DR PRIDE; Q8TDR4; -.
DR ProteomicsDB; 74333; -.
DR Antibodypedia; 34914; 71 antibodies from 19 providers.
DR DNASU; 140290; -.
DR Ensembl; ENST00000300258.8; ENSP00000300258.3; ENSG00000242220.9.
DR Ensembl; ENST00000674156.1; ENSP00000501209.1; ENSG00000242220.9.
DR GeneID; 140290; -.
DR KEGG; hsa:140290; -.
DR MANE-Select; ENST00000300258.8; ENSP00000300258.3; NM_144659.7; NP_653260.1.
DR UCSC; uc002ypw.5; human.
DR CTD; 140290; -.
DR DisGeNET; 140290; -.
DR GeneCards; TCP10L; -.
DR HGNC; HGNC:11657; TCP10L.
DR HPA; ENSG00000242220; Tissue enriched (testis).
DR MIM; 608365; gene.
DR neXtProt; NX_Q8TDR4; -.
DR OpenTargets; ENSG00000242220; -.
DR PharmGKB; PA36408; -.
DR VEuPathDB; HostDB:ENSG00000242220; -.
DR eggNOG; ENOG502TE43; Eukaryota.
DR GeneTree; ENSGT00530000063927; -.
DR InParanoid; Q8TDR4; -.
DR OMA; QGTHPED; -.
DR OrthoDB; 1079968at2759; -.
DR PhylomeDB; Q8TDR4; -.
DR PathwayCommons; Q8TDR4; -.
DR SignaLink; Q8TDR4; -.
DR BioGRID-ORCS; 140290; 8 hits in 1064 CRISPR screens.
DR GeneWiki; TCP10L; -.
DR GenomeRNAi; 140290; -.
DR Pharos; Q8TDR4; Tbio.
DR PRO; PR:Q8TDR4; -.
DR Proteomes; UP000005640; Chromosome 21.
DR RNAct; Q8TDR4; protein.
DR Bgee; ENSG00000242220; Expressed in left testis and 91 other tissues.
DR ExpressionAtlas; Q8TDR4; baseline and differential.
DR Genevisible; Q8TDR4; HS.
DR GO; GO:0005814; C:centriole; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0140297; F:DNA-binding transcription factor binding; IDA:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0043621; F:protein self-association; IDA:UniProtKB.
DR GO; GO:0003714; F:transcription corepressor activity; IDA:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR InterPro; IPR026581; TCP10_fam.
DR PANTHER; PTHR10331; PTHR10331; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation; Tumor suppressor.
FT CHAIN 1..215
FT /note="T-complex protein 10A homolog 1"
FT /id="PRO_0000072462"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 75..96
FT /note="Leucine-zipper"
FT REGION 150..215
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 69..110
FT /evidence="ECO:0000255"
FT COMPBIAS 150..166
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 185..200
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 145
FT /note="H -> R (in dbSNP:rs9622)"
FT /evidence="ECO:0000269|PubMed:10830953, ECO:0000269|Ref.3"
FT /id="VAR_016099"
FT VARIANT 194
FT /note="R -> H (in dbSNP:rs16989521)"
FT /id="VAR_049089"
FT MUTAGEN 82
FT /note="L->F: Greatly decreases in vitro transcription
FT inhibition activity."
FT /evidence="ECO:0000269|PubMed:14586771"
FT MUTAGEN 89
FT /note="L->P: Disrupts self-association."
FT /evidence="ECO:0000269|PubMed:17377852"
SQ SEQUENCE 215 AA; 23863 MW; CF3F74AB520F55FE CRC64;
MLAGQLEARD PKEGTHPEDP CPGAGAVMEK TAVAAEVLTE DCNTGEMPPL QQQIIRLHQE
LGRQKSLWAD VHGKLRSHID ALREQNMELR EKLRALQLQR WKARKKSAAS PHAGQESHTL
ALEPAFGKIS PLSADEETIP KYAGHKNQSA TLLGQRSSSN NSAPPKPMSL KIERISSWKT
PPQENRDKNL SRRRQDRRAT PTGRPTPCAE RRGGV
