BQMT1_ORYSJ
ID BQMT1_ORYSJ Reviewed; 330 AA.
AC Q6ZLD3; A0A0P0X3P8;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=2-methyl-6-phytyl-1,4-hydroquinone methyltransferase 1, chloroplastic;
DE EC=2.1.1.295;
DE AltName: Full=37 kDa inner envelope membrane protein;
DE Short=E37;
DE AltName: Full=Arsenite methyltransferase 2;
DE Short=OsArsM2;
DE AltName: Full=MPBQ/MSBQ methyltransferase;
DE AltName: Full=Protein VTE3 homolog;
DE Flags: Precursor;
GN Name=ARSM2; OrderedLocusNames=Os07g0179300, LOC_Os07g08200;
GN ORFNames=OJ1014_E09.7, OJ1361_E02.125;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Root;
RA Duan G., Rosen B.P., Zhu Y.;
RT "Arsenite methylation in rice.";
RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Involved in a key methylation step in both tocopherols
CC (vitamin E) and plastoquinone synthesis. Catalyzes the conversion of 2-
CC methyl-6-phytyl-1,4-hydroquinone (MPBQ) to 2,3-dimethyl-6-phytyl-1,4-
CC hydroquinone (DMPQ, a substrate for tocopherol cyclase), and 2-methyl-
CC 6-solanyl-1,4-benzoquinone (MSBQ) to plastoquinone (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-methyl-6-phytyl-1,4-benzene-1,4-diol + S-adenosyl-L-
CC methionine = 2,3-dimethyl-6-phytylbenzene-1,4-diol + H(+) + S-
CC adenosyl-L-homocysteine; Xref=Rhea:RHEA:37979, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:75920,
CC ChEBI:CHEBI:75921; EC=2.1.1.295;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-methyl-6-all-trans-nonaprenylbenzene-1,4-diol + S-adenosyl-
CC L-methionine = H(+) + plastoquinol-9 + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:37999, ChEBI:CHEBI:15378, ChEBI:CHEBI:28026,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:75402;
CC EC=2.1.1.295;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=6-geranylgeranyl-2-methylbenzene-1,4-diol + S-adenosyl-L-
CC methionine = 6-geranylgeranyl-2,3-dimethylbenzene-1,4-diol + H(+) +
CC S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:38007, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:75411,
CC ChEBI:CHEBI:75412; EC=2.1.1.295;
CC -!- PATHWAY: Cofactor biosynthesis; tocopherol biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. MPBQ/MBSQ MT family. {ECO:0000255|PROSITE-
CC ProRule:PRU01069}.
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DR EMBL; DQ514526; ABF60542.1; -; mRNA.
DR EMBL; AP003800; BAC83032.1; -; Genomic_DNA.
DR EMBL; AP003826; BAD30335.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT00307.1; -; Genomic_DNA.
DR RefSeq; XP_015647024.1; XM_015791538.1.
DR AlphaFoldDB; Q6ZLD3; -.
DR SMR; Q6ZLD3; -.
DR STRING; 4530.OS07T0179300-00; -.
DR PaxDb; Q6ZLD3; -.
DR PRIDE; Q6ZLD3; -.
DR EnsemblPlants; Os07t0179300-00; Os07t0179300-00; Os07g0179300.
DR GeneID; 107277102; -.
DR Gramene; Os07t0179300-00; Os07t0179300-00; Os07g0179300.
DR KEGG; osa:107277102; -.
DR eggNOG; KOG1540; Eukaryota.
DR HOGENOM; CLU_051421_0_0_1; -.
DR InParanoid; Q6ZLD3; -.
DR OMA; MAATYFV; -.
DR OrthoDB; 1178802at2759; -.
DR PlantReactome; R-OSA-1119287; Vitamin E biosynthesis.
DR UniPathway; UPA00160; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR Genevisible; Q6ZLD3; OS.
DR GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0102550; F:2-methyl-6-geranylgeranyl-1,4-benzoquinol methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0051741; F:2-methyl-6-phytyl-1,4-benzoquinone methyltransferase activity; IEA:InterPro.
DR GO; GO:0051742; F:2-methyl-6-solanyl-1,4-benzoquinone methyltransferase activity; IEA:RHEA.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0010189; P:vitamin E biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR041698; Methyltransf_25.
DR InterPro; IPR044649; MPBQ/MSBQ_MT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR031164; SAM_MPBQ_MSBQ_MT.
DR PANTHER; PTHR44516; PTHR44516; 1.
DR Pfam; PF13649; Methyltransf_25; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS51734; SAM_MPBQ_MSBQ_MT; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Methyltransferase; Plastid; Plastid inner membrane;
KW Reference proteome; S-adenosyl-L-methionine; Transferase; Transit peptide;
KW Transmembrane; Transmembrane helix.
FT TRANSIT 1..45
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 46..330
FT /note="2-methyl-6-phytyl-1,4-hydroquinone methyltransferase
FT 1, chloroplastic"
FT /id="PRO_0000422877"
FT TOPO_DOM 46..295
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 317..330
FT /note="Stromal"
FT /evidence="ECO:0000255"
FT REGION 107..116
FT /note="SAM motif I"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01069"
FT REGION 152..165
FT /note="SAM motif II"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01069"
FT REGION 193..206
FT /note="SAM motif III"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01069"
SQ SEQUENCE 330 AA; 37439 MW; 6A0CFC0C05829B45 CRC64;
MKEMVSSSTF RAPGGLGFLG PSKIGLIPLR NRSGVRSRVK YIAPKCAVSS ARPASQPRFI
QHKKEAFWFY RFLSIVYDHV INPGHWTEDM RDDALEPAEL YHHGLKVVDV GGGTGFTTLG
IVKHVDNENV TLLDQSPHQL EKARQKVALN GVNIIEGDAE DLPYPTDTFD RYVSAGSIEY
WPDPQRGIRE AYRVLKLGGV ACLIGPVHPT FWLSRFFADM WMLFPKEEEY IEWFQKAGFQ
DVKIKRIGPK WYRGVRRHGL IMGCSVTGVK RSSGDSPLQL GPKAEDVEKP VNPFTFIFRF
VMGTICASYY VLVPIYMWMK DQIVPKDQPI
