TCP4_DROME
ID TCP4_DROME Reviewed; 110 AA.
AC Q9VLR5; Q24026; Q4V3U5;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=RNA polymerase II transcriptional coactivator;
DE AltName: Full=p11;
GN Name=Ssb-c31a; ORFNames=CG8396;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=8559667; DOI=10.1093/nar/23.24.5073;
RA Lacoste J., Codani-Simonart S., Best-Belpomme M., Peronnet F.;
RT "Characterization and cloning of p11, a transrepressor of Drosophila
RT melanogaster retrotransposon 1731.";
RL Nucleic Acids Res. 23:5073-5079(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: General coactivator that functions cooperatively with TAFs
CC and mediates functional interactions between upstream activators and
CC the general transcriptional machinery. Binds single-stranded DNA (By
CC similarity). Binds specifically to the NssBF element, a short
CC nucleotide sequence of the 1731 retrotransposon, to repress promoter
CC activity. {ECO:0000250, ECO:0000269|PubMed:8559667}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:8559667}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically, no
CC expression is seen in larvae and weak expression in pupae.
CC {ECO:0000269|PubMed:8559667}.
CC -!- SIMILARITY: Belongs to the transcriptional coactivator PC4 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC46989.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U18971; AAC46989.1; ALT_FRAME; mRNA.
DR EMBL; AE014134; AAF52619.1; -; Genomic_DNA.
DR EMBL; BT023261; AAY55677.1; -; mRNA.
DR PIR; S66164; S66164.
DR RefSeq; NP_477136.1; NM_057788.4.
DR AlphaFoldDB; Q9VLR5; -.
DR SMR; Q9VLR5; -.
DR BioGRID; 60249; 25.
DR IntAct; Q9VLR5; 2.
DR STRING; 7227.FBpp0079211; -.
DR PaxDb; Q9VLR5; -.
DR PRIDE; Q9VLR5; -.
DR DNASU; 34120; -.
DR EnsemblMetazoa; FBtr0079590; FBpp0079211; FBgn0015299.
DR GeneID; 34120; -.
DR KEGG; dme:Dmel_CG8396; -.
DR CTD; 34120; -.
DR FlyBase; FBgn0015299; Ssb-c31a.
DR VEuPathDB; VectorBase:FBgn0015299; -.
DR eggNOG; KOG2712; Eukaryota.
DR GeneTree; ENSGT00940000166509; -.
DR HOGENOM; CLU_104273_1_2_1; -.
DR InParanoid; Q9VLR5; -.
DR OMA; LDQWNVL; -.
DR OrthoDB; 1616655at2759; -.
DR PhylomeDB; Q9VLR5; -.
DR SignaLink; Q9VLR5; -.
DR BioGRID-ORCS; 34120; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34120; -.
DR PRO; PR:Q9VLR5; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0015299; Expressed in secondary oocyte and 30 other tissues.
DR ExpressionAtlas; Q9VLR5; baseline and differential.
DR Genevisible; Q9VLR5; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0003697; F:single-stranded DNA binding; IDA:FlyBase.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:FlyBase.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR Gene3D; 2.30.31.10; -; 1.
DR InterPro; IPR003173; PC4_C.
DR InterPro; IPR009044; ssDNA-bd_transcriptional_reg.
DR InterPro; IPR045125; Sub1/Tcp4-like.
DR PANTHER; PTHR13215; PTHR13215; 1.
DR Pfam; PF02229; PC4; 1.
DR SUPFAM; SSF54447; SSF54447; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..110
FT /note="RNA polymerase II transcriptional coactivator"
FT /id="PRO_0000215946"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..37
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 110 AA; 12189 MW; 03EF363E88CB32E0 CRC64;
MPKTKKKDSS SDSDSGPDDR IKPASKKAKE SDAPNSDPKD SGENGATSWT LEGLRQVRIN
EFRGRKSVDI REFYDKGGQI LPGKKGISLS LIQWKKLLEV AEEVTRAIEN
