ID   TCPE_VIBC3              Reviewed;         340 AA.
AC   A5F382; C3LYK1; P29487; Q9JQ06;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Toxin coregulated pilus biosynthesis protein E;
DE   AltName: Full=TCP pilus biosynthesis protein TcpE;
GN   Name=tcpE; OrderedLocusNames=VC0395_A0361, VC395_0852;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11179381; DOI=10.1128/iai.69.3.1947-1952.2001;
RA   Karaolis D.K.R., Lan R., Kaper J.B., Reeves P.R.;
RT   "Comparison of Vibrio cholerae pathogenicity islands in sixth and seventh
RT   pandemic strains.";
RL   Infect. Immun. 69:1947-1952(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
CC   -!- FUNCTION: Probably involved in cholera toxin receptor (GM1) interaction
CC       in order to bring the cells within close proximity of the ganglioside
CC       for efficient toxin delivery. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GSP F family. {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-15 is the initiator.
CC       {ECO:0000305}.
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DR   EMBL; AF325733; AAK20763.1; -; Genomic_DNA.
DR   EMBL; CP000627; ABQ21872.1; -; Genomic_DNA.
DR   EMBL; CP001235; ACP08867.1; -; Genomic_DNA.
DR   RefSeq; WP_000691946.1; NZ_JAACZH010000023.1.
DR   AlphaFoldDB; A5F382; -.
DR   SMR; A5F382; -.
DR   STRING; 345073.VC395_0852; -.
DR   EnsemblBacteria; ABQ21872; ABQ21872; VC0395_A0361.
DR   GeneID; 57739538; -.
DR   KEGG; vco:VC0395_A0361; -.
DR   KEGG; vcr:VC395_0852; -.
DR   PATRIC; fig|345073.21.peg.824; -.
DR   eggNOG; COG1459; Bacteria.
DR   HOGENOM; CLU_063664_0_0_6; -.
DR   OMA; YQFCADM; -.
DR   Proteomes; UP000000249; Chromosome 2.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   Gene3D; 1.20.81.30; -; 2.
DR   InterPro; IPR003004; GspF/PilC.
DR   InterPro; IPR001992; T2SS_GspF/T4SS_PilC_CS.
DR   InterPro; IPR018076; T2SS_GspF_dom.
DR   InterPro; IPR042094; T2SS_GspF_sf.
DR   PANTHER; PTHR30012; PTHR30012; 1.
DR   Pfam; PF00482; T2SSF; 2.
DR   PROSITE; PS00874; T2SP_F; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..340
FT                   /note="Toxin coregulated pilus biosynthesis protein E"
FT                   /id="PRO_0000321852"
FT   TRANSMEM        108..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        312..333
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   340 AA;  38068 MW;  96DE4B3B7DAE18E4 CRC64;
     MKIISKKYRL ELYSMLVDLL NDNIPLYDAL NKIQNEGVGI YDKNFIKSIE LIKDRMKSNS
     SLTDALTGLI PDKEVLMINV AENSGKISSG IAAIRKNIID ADEIKSKAIS SMITPSVMLI
     VTMVVIAGYS VKVFPTFESV LPVSRWPGVT QALYNLGFSL YEGLWIKVLI FVAIFITILV
     FMSKNITGNF RDGFLDKLPP FNFVKHIAAT EFLANMSMLL DSRVPFKEGL DIVDHKTTRW
     LSSHLQRMKA NMQEGLDYKQ ALDTNLLDKK MLLTMAVYSE LPNFSDVMQK LAIEANINLH
     KKIATLAGVM KNISLITLAL SVIWIFGAIF SLVDKLSSSL