TCPF_VIBCH
ID TCPF_VIBCH Reviewed; 338 AA.
AC P0C6Q5; P29488; Q9KTQ9;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 1.
DT 03-AUG-2022, entry version 50.
DE RecName: Full=Toxin coregulated pilus biosynthesis protein F;
DE AltName: Full=TCP pilus biosynthesis protein TcpF;
DE Flags: Precursor;
GN Name=tcpF; OrderedLocusNames=VC_0837;
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1400247; DOI=10.1128/jb.174.21.6974-6980.1992;
RA Higgins D.E., Nazareno E., DiRita V.J.;
RT "The virulence gene activator ToxT from Vibrio cholerae is a member of the
RT AraC family of transcriptional activators.";
RL J. Bacteriol. 174:6974-6980(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Classical Inaba Z17561 / Serotype O1;
RX PubMed=8097178; DOI=10.1016/0378-1119(93)90589-u;
RA Ogierman M.A., Zabihi S., Mourtzios L., Manning P.A.;
RT "Genetic organization and sequence of the promoter-distal region of the tcp
RT gene cluster of Vibrio cholerae.";
RL Gene 126:51-60(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8097177; DOI=10.1016/0378-1119(93)90588-t;
RA Kaufman M.R., Shaw C.E., Jones I.D., Taylor R.K.;
RT "Biogenesis and regulation of the Vibrio cholerae toxin-coregulated pilus:
RT analogies to other virulence factor secretory systems.";
RL Gene 126:43-49(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
CC -!- FUNCTION: Involved in TCP pilus biogenesis. May be a channel protein.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Peripheral
CC membrane protein {ECO:0000305}.
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DR EMBL; L01623; AAA27566.1; -; Genomic_DNA.
DR EMBL; X64098; CAA45464.1; -; Genomic_DNA.
DR EMBL; M93963; AAA27564.1; -; Genomic_DNA.
DR EMBL; AE003852; AAF94000.1; -; Genomic_DNA.
DR PIR; JN0526; JN0526.
DR RefSeq; NP_230485.1; NC_002505.1.
DR PDB; 3OC5; X-ray; 2.40 A; A=21-338.
DR PDB; 3OC8; X-ray; 2.10 A; A=206-338.
DR PDBsum; 3OC5; -.
DR PDBsum; 3OC8; -.
DR AlphaFoldDB; P0C6Q5; -.
DR SMR; P0C6Q5; -.
DR STRING; 243277.VC_0837; -.
DR PRIDE; P0C6Q5; -.
DR DNASU; 2614504; -.
DR EnsemblBacteria; AAF94000; AAF94000; VC_0837.
DR KEGG; vch:VC_0837; -.
DR PATRIC; fig|243277.26.peg.798; -.
DR HOGENOM; CLU_839222_0_0_6; -.
DR OMA; KPYQPSD; -.
DR BioCyc; VCHO:VC0837-MON; -.
DR EvolutionaryTrace; P0C6Q5; -.
DR Proteomes; UP000000584; Chromosome 1.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 2.60.40.3240; -; 1.
DR InterPro; IPR009405; TcpF.
DR InterPro; IPR043125; TcpF_C.
DR Pfam; PF06340; TcpF; 1.
DR PIRSF; PIRSF020763; TcpF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell outer membrane; Membrane; Reference proteome; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..338
FT /note="Toxin coregulated pilus biosynthesis protein F"
FT /id="PRO_0000022477"
FT CONFLICT 21..23
FT /note="FND -> INE (in Ref. 2; CAA45464)"
FT /evidence="ECO:0000305"
FT CONFLICT 40
FT /note="D -> E (in Ref. 2; CAA45464)"
FT /evidence="ECO:0000305"
FT CONFLICT 236
FT /note="N -> S (in Ref. 4; AAF94000)"
FT /evidence="ECO:0000305"
FT CONFLICT 281
FT /note="N -> G (in Ref. 4; AAF94000)"
FT /evidence="ECO:0000305"
FT TURN 47..50
FT /evidence="ECO:0007829|PDB:3OC5"
FT HELIX 51..56
FT /evidence="ECO:0007829|PDB:3OC5"
FT HELIX 61..68
FT /evidence="ECO:0007829|PDB:3OC5"
FT HELIX 78..82
FT /evidence="ECO:0007829|PDB:3OC5"
FT HELIX 96..104
FT /evidence="ECO:0007829|PDB:3OC5"
FT HELIX 108..114
FT /evidence="ECO:0007829|PDB:3OC5"
FT HELIX 116..118
FT /evidence="ECO:0007829|PDB:3OC5"
FT HELIX 132..143
FT /evidence="ECO:0007829|PDB:3OC5"
FT HELIX 149..154
FT /evidence="ECO:0007829|PDB:3OC5"
FT STRAND 156..164
FT /evidence="ECO:0007829|PDB:3OC5"
FT STRAND 167..175
FT /evidence="ECO:0007829|PDB:3OC5"
FT TURN 190..192
FT /evidence="ECO:0007829|PDB:3OC5"
FT STRAND 200..204
FT /evidence="ECO:0007829|PDB:3OC5"
FT STRAND 228..234
FT /evidence="ECO:0007829|PDB:3OC8"
FT HELIX 235..240
FT /evidence="ECO:0007829|PDB:3OC8"
FT STRAND 248..256
FT /evidence="ECO:0007829|PDB:3OC8"
FT TURN 260..262
FT /evidence="ECO:0007829|PDB:3OC8"
FT STRAND 269..274
FT /evidence="ECO:0007829|PDB:3OC8"
FT STRAND 286..292
FT /evidence="ECO:0007829|PDB:3OC8"
FT HELIX 295..297
FT /evidence="ECO:0007829|PDB:3OC8"
FT HELIX 301..306
FT /evidence="ECO:0007829|PDB:3OC8"
FT STRAND 313..320
FT /evidence="ECO:0007829|PDB:3OC8"
FT HELIX 335..337
FT /evidence="ECO:0007829|PDB:3OC8"
SQ SEQUENCE 338 AA; 38211 MW; 82D6E9B660506939 CRC64;
MRYKKTLMLS IMITSFNSFA FNDNYSSTST VYATSNEATD SRGSEHLRYP YLECIKIGMS
RDYLENCVKV SFPTSQDMFY DAYPSTESDG AKTRTKEDFS ARLLAGDYDS LQKLYIDFYL
AQTTFDWEIP TRDQIETLVN YANEGKLSTA LNQEYITGRF LTKENGRYDI VNVGGVPDNT
PVKLPAIVSK RGLMGTTSVV NAIPNEIYPH IKVYEGTLSR LKPGGAMIAV LEYDVNELSK
HGYTNLWDVQ FKVLVGVPHA ETGVIYDPVY EETVKPYQPS NNLTGKKLYN VSTNDMHNGY
KWSNTMFSNS NYKTQILLTK GDGSGVKLYS KAYSENFK
