ID   TCPI_VIBCH              Reviewed;         620 AA.
AC   P0C6D8; P29486; Q9KTR5;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Toxin coregulated pilus biosynthesis protein I;
DE   AltName: Full=TCP pilus biosynthesis protein TcpI;
GN   Name=tcpI; Synonyms=tagB; OrderedLocusNames=VC_0825;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-406.
RC   STRAIN=Classical Inaba Z17561 / Serotype O1;
RX   PubMed=8621096; DOI=10.1016/0378-1119(95)00744-x;
RA   Ogierman M.A., Voss E., Meaney C., Faast R., Attridge S.R., Manning P.A.;
RT   "Comparison of the promoter proximal regions of the toxin-co-regulated tcp
RT   gene cluster in classical and El Tor strains of Vibrio cholerae O1.";
RL   Gene 170:9-16(1996).
CC   -!- FUNCTION: May function as an environmental regulator of TCP biogenesis.
CC       Negatively regulates the synthesis of the major pilin subunit of TCP
CC       (TcpA) (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Peripheral
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the methyl-accepting chemotaxis (MCP) protein
CC       family. {ECO:0000305}.
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DR   EMBL; AE003852; AAF93988.1; -; Genomic_DNA.
DR   EMBL; X64098; CAA45452.1; -; Genomic_DNA.
DR   PIR; D82274; D82274.
DR   RefSeq; NP_230473.1; NC_002505.1.
DR   RefSeq; WP_000591844.1; NZ_LT906614.1.
DR   AlphaFoldDB; P0C6D8; -.
DR   SMR; P0C6D8; -.
DR   STRING; 243277.VC_0825; -.
DR   DNASU; 2614492; -.
DR   EnsemblBacteria; AAF93988; AAF93988; VC_0825.
DR   GeneID; 57739528; -.
DR   KEGG; vch:VC_0825; -.
DR   PATRIC; fig|243277.26.peg.786; -.
DR   eggNOG; COG0840; Bacteria.
DR   HOGENOM; CLU_000445_107_19_6; -.
DR   OMA; KEMKREW; -.
DR   BioCyc; VCHO:VC0825-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006935; P:chemotaxis; IEA:UniProt.
DR   GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR   InterPro; IPR004089; MCPsignal_dom.
DR   InterPro; IPR029151; Sensor-like_sf.
DR   Pfam; PF00015; MCPsignal; 1.
DR   SMART; SM00283; MA; 1.
DR   SUPFAM; SSF103190; SSF103190; 1.
DR   PROSITE; PS50111; CHEMOTAXIS_TRANSDUC_2; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Methylation;
KW   Reference proteome; Transducer.
FT   CHAIN           1..620
FT                   /note="Toxin coregulated pilus biosynthesis protein I"
FT                   /id="PRO_0000110563"
FT   DOMAIN          344..580
FT                   /note="Methyl-accepting transducer"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00284"
FT   CONFLICT        18
FT                   /note="L -> S (in Ref. 2; CAA45452)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        63
FT                   /note="T -> I (in Ref. 2; CAA45452)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        302
FT                   /note="A -> S (in Ref. 2; CAA45452)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        402..406
FT                   /note="ETAQK -> RRPKN (in Ref. 2; CAA45452)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   620 AA;  69028 MW;  9DBEF34A042BBF91 CRC64;
     MIKKIISVFL LLACIITLAF TAFFYHSKLS DQTKSISSLS SQQAQERLQS YQDSLDFYKK
     LNTSLSVAIA NSLRDKAVEE LNAIALRIQE NHGFIGVTFA SLDGTMFTDI GTLDWNAKTL
     RRDWFVKTVE LGTKHYTAFD IDKTTGQHVL TIATPVYVGN DIVGSVALDI AGDQIASPNG
     SGMFMMTDRN FNVFASDLTH STLIGKDLTK EKPLFKNLVS GQYVTFSDAD SHWFAVSQTE
     IDGENKLFTI IDIQQIVQTY KRDIQLIIAG FSGFSCVMLI GLYWVLSKEL SGVRQIREWI
     LALSDGQIKE RRPIKFHNEL DTIAQSLENL QFRLLDVVRN SHRTMNDLSI KQTDITYSIE
     GNTNNSQQEL GLIEQVATAT TQLSCTSFDV MQQAQSAELN AETAQKLIAE SHDIIDSSSK
     QTEMVTLSIH ESQQIINQLR EFSDNISSVT DVINNISDQT NLLALNAAIE AARAGEQGRG
     FAVVADEVRS LAVKTQQSTI DIQGIILKLQ EQSQLADQVM TRNVSLIHET QVANRALIAS
     FNLISDKVLE ISNINSIVST AANEQKIVTE DVAKQMEDIR YLVQENLSAM ERTKQANQNI
     SDLTTNLNDA LSFFKIELTS