ID   TCPK_CLAP2              Reviewed;         222 AA.
AC   M1W855;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2013, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Gamma-glutamyl cyclotransferase gliK {ECO:0000250|UniProtKB:E9R9Y3};
DE            Short=GGCT gliK {ECO:0000250|UniProtKB:E9R9Y3};
DE            EC=4.3.2.9 {ECO:0000305|PubMed:27390873};
DE   AltName: Full=Thiocalpurine biosynthesis protein K {ECO:0000303|PubMed:27390873};
GN   Name=tcpK {ECO:0000303|PubMed:27390873}; ORFNames=CPUR_02674;
OS   Claviceps purpurea (strain 20.1) (Ergot fungus) (Sphacelia segetum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Clavicipitaceae; Claviceps.
OX   NCBI_TaxID=1111077;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=20.1;
RX   PubMed=23468653; DOI=10.1371/journal.pgen.1003323;
RA   Schardl C.L., Young C.A., Hesse U., Amyotte S.G., Andreeva K., Calie P.J.,
RA   Fleetwood D.J., Haws D.C., Moore N., Oeser B., Panaccione D.G.,
RA   Schweri K.K., Voisey C.R., Farman M.L., Jaromczyk J.W., Roe B.A.,
RA   O'Sullivan D.M., Scott B., Tudzynski P., An Z., Arnaoudova E.G.,
RA   Bullock C.T., Charlton N.D., Chen L., Cox M., Dinkins R.D., Florea S.,
RA   Glenn A.E., Gordon A., Gueldener U., Harris D.R., Hollin W., Jaromczyk J.,
RA   Johnson R.D., Khan A.K., Leistner E., Leuchtmann A., Li C., Liu J., Liu J.,
RA   Liu M., Mace W., Machado C., Nagabhyru P., Pan J., Schmid J., Sugawara K.,
RA   Steiner U., Takach J.E., Tanaka E., Webb J.S., Wilson E.V., Wiseman J.L.,
RA   Yoshida R., Zeng Z.;
RT   "Plant-symbiotic fungi as chemical engineers: Multi-genome analysis of the
RT   Clavicipitaceae reveals dynamics of alkaloid loci.";
RL   PLoS Genet. 9:E1003323-E1003323(2013).
RN   [2]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=27390873; DOI=10.1371/journal.pone.0158945;
RA   Dopstadt J., Neubauer L., Tudzynski P., Humpf H.U.;
RT   "The epipolythiodiketopiperazine gene cluster in Claviceps purpurea:
RT   dysfunctional cytochrome P450 enzyme prevents formation of the previously
RT   unknown clapurines.";
RL   PLoS ONE 11:E0158945-E0158945(2016).
CC   -!- FUNCTION: Gamma-glutamyl cyclotransferase; part of the gene cluster
CC       that mediates the biosynthesis of an unusual class of
CC       epipolythiodioxopiperazines (ETPs) lacking the reactive thiol group
CC       important for toxicity (PubMed:27390873). Firstly, L-tyrosine is
CC       prenylated by tcpD, before undergoing condensation with L-glycine in a
CC       reaction catalyzed by the NRPS tcpP leading to the diketopiperazine
CC       (DKP) backbone (PubMed:27390873). Afterwards the alpha-carbon of
CC       tyrosine is oxidized by the cytochrome P450 tcpC to form a hydroxyl
CC       group (PubMed:27390873). However, in contrast other ETP biosynthesis
CC       pathways studied so far, tcpC is not able to bishydroxylate the DKP at
CC       both alpha-carbon positions, but hydroxylates the alpha-carbon of the
CC       tyrosine part and the nitrogen of the glycine part (PubMed:27390873).
CC       The next steps involve an alpha,beta-elimination reaction catalyzed by
CC       tcpI, a methylation by the methyltransferase tcpN the action of the
CC       four enzyme cascade tcpG/K/J/I (PubMed:27390873). Due to a
CC       dysfunctional cytochrome P450 monooxygenase tcpC, the pathway leads to
CC       the biosynthesis of probable non-toxic metabolites lacking the reactive
CC       thiol group (PubMed:27390873). {ECO:0000269|PubMed:27390873}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an alpha-(gamma-L-glutamyl)-L-amino acid = 5-oxo-L-proline +
CC         an L-alpha-amino acid; Xref=Rhea:RHEA:20505, ChEBI:CHEBI:58402,
CC         ChEBI:CHEBI:59869, ChEBI:CHEBI:71304; EC=4.3.2.9;
CC         Evidence={ECO:0000305|PubMed:27390873};
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000305|PubMed:27390873}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Expression is positively regulated by the thioclapurine
CC       cluster-specific transcription factor tcpZ (PubMed:27390873).
CC       {ECO:0000269|PubMed:27390873}.
CC   -!- SIMILARITY: Belongs to the class-I pyridoxal-phosphate-dependent
CC       aminotransferase family. {ECO:0000305}.
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DR   EMBL; CAGA01000011; CCE28983.1; -; Genomic_DNA.
DR   AlphaFoldDB; M1W855; -.
DR   SMR; M1W855; -.
DR   STRING; 1111077.M1W855; -.
DR   EnsemblFungi; CCE28983; CCE28983; CPUR_02674.
DR   VEuPathDB; FungiDB:CPUR_02674; -.
DR   eggNOG; ENOG502SBD2; Eukaryota.
DR   HOGENOM; CLU_030506_0_0_1; -.
DR   OrthoDB; 1484710at2759; -.
DR   Proteomes; UP000016801; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0003839; F:gamma-glutamylcyclotransferase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR017939; G-Glutamylcylcotransferase.
DR   PANTHER; PTHR12935; PTHR12935; 1.
PE   2: Evidence at transcript level;
KW   Lyase; Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..222
FT                   /note="Gamma-glutamyl cyclotransferase gliK"
FT                   /id="PRO_0000437728"
FT   TRANSMEM        154..174
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        187..207
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   222 AA;  24889 MW;  BE7888ED08C6B39C CRC64;
     MSASVFIERR KIRPLRTEAA CIPTHALCFN VLGIPYMDPG NGGIRPLNPD DSDATACVHG
     VAYLLTSDDL KKVILSEGGG IAYQVARLNA KLLLDDSPIV VDTLIGRHNV DASNERLPSA
     RYIGVLTRGA NELNLPLSYQ RRLAEQPIYQ PKSGWWFQLG VALFLWPWTR AAIITERLVY
     KHQGPDGHVP AWFLFIFDCL LWLMWAQHDY IHGPIFGRGD GR