TCPN_VIBCH
ID TCPN_VIBCH Reviewed; 276 AA.
AC P0C6D6; P29492; Q9KTQ8;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=TCP pilus virulence regulatory protein;
GN Name=tcpN; Synonyms=toxT; OrderedLocusNames=VC_0838;
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Classical Inaba Z17561 / Serotype O1;
RX PubMed=1352761; DOI=10.1016/0378-1119(92)90634-2;
RA Ogierman M.A., Manning P.A.;
RT "Homology of TcpN, a putative regulatory protein of Vibrio cholerae, to the
RT AraC family of transcriptional activators.";
RL Gene 116:93-97(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 25870 / Classical Inaba 569B / Serotype O1;
RX PubMed=1400247; DOI=10.1128/jb.174.21.6974-6980.1992;
RA Higgins D.E., Nazareno E., DiRita V.J.;
RT "The virulence gene activator ToxT from Vibrio cholerae is a member of the
RT AraC family of transcriptional activators.";
RL J. Bacteriol. 174:6974-6980(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22.
RX PubMed=8097177; DOI=10.1016/0378-1119(93)90588-t;
RA Kaufman M.R., Shaw C.E., Jones I.D., Taylor R.K.;
RT "Biogenesis and regulation of the Vibrio cholerae toxin-coregulated pilus:
RT analogies to other virulence factor secretory systems.";
RL Gene 126:43-49(1993).
CC -!- FUNCTION: Probable regulatory protein for the tcp operon.
CC -!- INTERACTION:
CC P0C6D6; P0C6D6: tcpN; NbExp=2; IntAct=EBI-15621125, EBI-15621125;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
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DR EMBL; X64098; CAA45465.1; -; Genomic_DNA.
DR EMBL; M93963; AAA27565.1; -; Genomic_DNA.
DR EMBL; AE003852; AAF94001.1; -; Genomic_DNA.
DR EMBL; L01623; AAA27567.1; -; Genomic_DNA.
DR PIR; B45247; B45247.
DR PIR; C82273; C82273.
DR PIR; S23274; S23274.
DR RefSeq; NP_230486.1; NC_002505.1.
DR RefSeq; WP_000579582.1; NZ_LT906614.1.
DR AlphaFoldDB; P0C6D6; -.
DR SMR; P0C6D6; -.
DR DIP; DIP-60864N; -.
DR STRING; 243277.VC_0838; -.
DR PRIDE; P0C6D6; -.
DR DNASU; 2614505; -.
DR EnsemblBacteria; AAF94001; AAF94001; VC_0838.
DR GeneID; 57739540; -.
DR KEGG; vch:VC_0838; -.
DR PATRIC; fig|243277.26.peg.799; -.
DR eggNOG; COG2207; Bacteria.
DR HOGENOM; CLU_1004526_0_0_6; -.
DR OMA; EIDECIV; -.
DR BioCyc; VCHO:VC0838-MON; -.
DR Proteomes; UP000000584; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR Gene3D; 1.10.10.1310; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR InterPro; IPR044875; ToxT_HTH1.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR Pfam; PF00165; HTH_AraC; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation; Virulence.
FT CHAIN 1..276
FT /note="TCP pilus virulence regulatory protein"
FT /id="PRO_0000194587"
FT DOMAIN 172..269
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 189..210
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 236..259
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT CONFLICT 65
FT /note="S -> A (in Ref. 2; AAA27567)"
FT /evidence="ECO:0000305"
FT CONFLICT 250
FT /note="Y -> N (in Ref. 1; CAA45465)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 276 AA; 32055 MW; 0702B68C99680757 CRC64;
MIGKKSFQTN VYRMSKFDTY IFNNLYINDY KMFWIDSGIA KLIDKNCLVS YEINSSSIIL
LKKNSIQRFS LTSLSDENIN VSVITISDSF IRSLKSYILG DLMIRNLYSE NKDLLLWNCE
HNDIAVLSEV VNGFREINYS DEFLKVFFSG FFSKVEKKYN SIFITDDLDA MEKISCLVKS
DITRNWRWAD ICGELRTNRM ILKKELESRG VKFRELINSI RISYSISLMK TGEFKIKQIA
YQSGFASVSY FSTVFKSTMN VAPSEYLFML TGVAEK
