TCR1_ECOLX
ID TCR1_ECOLX Reviewed; 399 AA.
AC P02982;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 25-MAY-2022, entry version 144.
DE RecName: Full=Tetracycline resistance protein, class A;
DE Short=TetA(A);
GN Name=tetA;
OS Escherichia coli.
OG Plasmid RP1, and Plasmid pLV501.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=RP1; TRANSPOSON=Tn1721;
RX PubMed=6310527; DOI=10.1093/nar/11.17.6089;
RA Waters S.H., Rogowsky P., Grinsted J., Altenbuchner J., Schmitt R.;
RT "The tetracycline resistance determinants of RP1 and Tn1721: nucleotide
RT sequence analysis.";
RL Nucleic Acids Res. 11:6089-6105(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TRANSPOSON=Tn1721;
RX PubMed=1312499; DOI=10.1016/0378-1119(92)90597-i;
RA Allmeier H., Cresnar B., Greck M., Schmitt R.;
RT "Complete nucleotide sequence of Tn1721: gene organization and a novel gene
RT product with features of a chemotaxis protein.";
RL Gene 111:11-20(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 85-399.
RC PLASMID=pLV501;
RA Fletcher J.N., Hart C.A., Batt R.M., Saunders J.R.;
RT "The epithelial cell invasive determinant of enteropathogenic Escherichia
RT coli plasmid pLV501 is encoded on Tn4355, a novel Tn21-type transposon.";
RL Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Resistance to tetracycline by an active tetracycline efflux.
CC This is an energy-dependent process that decreases the accumulation of
CC the antibiotic in whole cells. This protein functions as a metal-
CC tetracycline/H(+) antiporter.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC family. {ECO:0000305}.
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DR EMBL; X00006; CAA24909.1; -; Genomic_DNA.
DR EMBL; X61367; CAA43643.1; -; Genomic_DNA.
DR EMBL; L29404; AAA83545.2; -; Genomic_DNA.
DR PIR; A03509; YTECR1.
DR PIR; JQ1479; JQ1479.
DR RefSeq; NP_957551.1; NC_005327.1.
DR RefSeq; WP_000804064.1; NZ_WVVZ01000065.1.
DR RefSeq; YP_009060101.1; NC_024956.1.
DR RefSeq; YP_190205.1; NC_006671.1.
DR AlphaFoldDB; P02982; -.
DR SMR; P02982; -.
DR TCDB; 2.A.1.2.4; the major facilitator superfamily (mfs).
DR GeneID; 58463759; -.
DR OMA; VRAGSWK; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004734; Multidrug-R.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR InterPro; IPR001958; Tet-R_TetA/multi-R_MdtG.
DR Pfam; PF07690; MFS_1; 1.
DR PRINTS; PR01035; TCRTETA.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00880; 2_A_01_02; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Antiport; Cell inner membrane; Cell membrane;
KW Hydrogen ion transport; Ion transport; Membrane; Plasmid; Transmembrane;
KW Transmembrane helix; Transport; Transposable element.
FT CHAIN 1..399
FT /note="Tetracycline resistance protein, class A"
FT /id="PRO_0000173392"
FT TRANSMEM 7..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..121
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..225
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..267
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 302..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..358
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..390
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 5
FT /note="R -> I (in Ref. 1; CAA24909)"
FT /evidence="ECO:0000305"
FT CONFLICT 55
FT /note="M -> V (in Ref. 1; CAA24909)"
FT /evidence="ECO:0000305"
FT CONFLICT 75
FT /note="V -> I (in Ref. 1; CAA24909)"
FT /evidence="ECO:0000305"
FT CONFLICT 84
FT /note="A -> T (in Ref. 1; CAA24909)"
FT /evidence="ECO:0000305"
FT CONFLICT 201..203
FT /note="ASF -> SFV (in Ref. 1; CAA24909)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 399 AA; 42241 MW; 298427E6B5478374 CRC64;
MKPNRPLIVI LSTVALDAVG IGLIMPVLPG LLRDLVHSND VTAHYGILLA LYALMQFACA
PVLGALSDRF GRRPVLLVSL AGAAVDYAIM ATAPFLWVLY IGRIVAGITG ATGAVAGAYI
ADITDGDERA RHFGFMSACF GFGMVAGPVL GGLMGGFSPH APFFAAAALN GLNFLTGCFL
LPESHKGERR PLRREALNPL ASFRWARGMT VVAALMAVFF IMQLVGQVPA ALWVIFGEDR
FHWDATTIGI SLAAFGILHS LAQAMITGPV AARLGERRAL MLGMIADGTG YILLAFATRG
WMAFPIMVLL ASGGIGMPAL QAMLSRQVDE ERQGQLQGSL AALTSLTSIV GPLLFTAIYA
ASITTWNGWA WIAGAALYLL CLPALRRGLW SGAGQRADR
