TCRA1_MYCTU
ID TCRA1_MYCTU Reviewed; 253 AA.
AC O07776; L0T499;
DT 09-FEB-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 25-MAY-2022, entry version 136.
DE RecName: Full=Transcriptional regulatory protein TcrA;
GN Name=tcrA; OrderedLocusNames=Rv0602c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP FUNCTION.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=17434492; DOI=10.1016/j.febslet.2007.03.089;
RA Shrivastava R., Ghosh A.K., Das A.K.;
RT "Probing the nucleotide binding and phosphorylation by the histidine kinase
RT of a novel three-protein two-component system from Mycobacterium
RT tuberculosis.";
RL FEBS Lett. 581:1903-1909(2007).
RN [3]
RP IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA Raman K., Yeturu K., Chandra N.;
RT "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT through an interactome, reactome and genome-scale structural analysis.";
RL BMC Syst. Biol. 2:109-109(2008).
RN [4]
RP INTERACTION WITH HK2.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=19246748; DOI=10.1099/mic.0.019059-0;
RA Shrivastava R., Ghosh A.K., Das A.K.;
RT "Intra- and intermolecular domain interactions among novel two-component
RT system proteins coded by Rv0600c, Rv0601c and Rv0602c of Mycobacterium
RT tuberculosis.";
RL Microbiology 155:772-779(2009).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Member of the three-protein two-component system
CC HK1/HK2/TcrA. {ECO:0000269|PubMed:17434492}.
CC -!- SUBUNIT: Interacts with HK2. {ECO:0000269|PubMed:19246748}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Phosphorylated by HK2.
CC -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
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DR EMBL; AL123456; CCP43341.1; -; Genomic_DNA.
DR PIR; E70909; E70909.
DR RefSeq; NP_215116.1; NC_000962.3.
DR RefSeq; WP_003403148.1; NZ_KK339370.1.
DR AlphaFoldDB; O07776; -.
DR SMR; O07776; -.
DR STRING; 83332.Rv0602c; -.
DR PaxDb; O07776; -.
DR DNASU; 887870; -.
DR GeneID; 887870; -.
DR KEGG; mtu:Rv0602c; -.
DR TubercuList; Rv0602c; -.
DR eggNOG; COG0745; Bacteria.
DR InParanoid; O07776; -.
DR OMA; GHVVECS; -.
DR PhylomeDB; O07776; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IDA:MTBBASE.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0070298; P:negative regulation of phosphorelay signal transduction system; IDA:MTBBASE.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..253
FT /note="Transcriptional regulatory protein TcrA"
FT /id="PRO_0000391082"
FT DOMAIN 24..138
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 146..244
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 73
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 253 AA; 28170 MW; E4F0DA2EA8E1BA18 CRC64;
MADETTMRAG RGPGRACGRV SGVRILVVED EPKMTALLAR ALTEEGHTVD TVADGRHAVA
AVDGGDYDAV VLDVMLPGID GFEVCARLRR QRVWTPVLML TARGAVTDRI AGLDGGADDY
LTKPFNLDEL FARLRALSRR GPIPRPPTLE AGDLRLDPSE HRVWRADTEI RLSHKEFTLL
EALIRRPGIV HTRAQLLERC WDAAYEARSN IVDVYIRYLR DKIDRPFGVT SLETIRGAGY
RLRKDGGRHA LPR
