TCRB_BACSU
ID TCRB_BACSU Reviewed; 458 AA.
AC P23054;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Tetracycline resistance protein;
GN Name=tetB; Synonyms=tet; OrderedLocusNames=BSU40770;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=2844262; DOI=10.1016/0167-4781(88)90142-x;
RA Sakaguchi R., Amano H., Shishido K.;
RT "Nucleotide sequence homology of the tetracycline-resistance determinant
RT naturally maintained in Bacillus subtilis Marburg 168 chromosome and the
RT tetracycline-resistance gene of B. subtilis plasmid pNS1981.";
RL Biochim. Biophys. Acta 950:441-444(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA Ogasawara N., Nakai S., Yoshikawa H.;
RT "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis
RT chromosome containing the replication origin.";
RL DNA Res. 1:1-14(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
CC -!- FUNCTION: Resistance to tetracycline by an active tetracycline efflux.
CC This is an energy-dependent process that decreases the accumulation of
CC the antibiotic in whole cells. This protein functions as a metal-
CC tetracycline/H(+) antiporter.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC family. {ECO:0000305}.
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DR EMBL; X08034; CAA30827.1; -; Genomic_DNA.
DR EMBL; D26185; BAA05208.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB16114.1; -; Genomic_DNA.
DR PIR; JT0427; YTBSY8.
DR RefSeq; NP_391957.1; NC_000964.3.
DR RefSeq; WP_003242953.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; P23054; -.
DR SMR; P23054; -.
DR STRING; 224308.BSU40770; -.
DR TCDB; 2.A.1.3.16; the major facilitator superfamily (mfs).
DR PaxDb; P23054; -.
DR PRIDE; P23054; -.
DR EnsemblBacteria; CAB16114; CAB16114; BSU_40770.
DR GeneID; 937890; -.
DR KEGG; ag:CAA30827; -.
DR KEGG; bsu:BSU40770; -.
DR PATRIC; fig|224308.179.peg.4419; -.
DR eggNOG; COG2814; Bacteria.
DR InParanoid; P23054; -.
DR OMA; ESRAMNH; -.
DR PhylomeDB; P23054; -.
DR BioCyc; BSUB:BSU40770-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Antiport; Cell membrane; Hydrogen ion transport;
KW Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..458
FT /note="Tetracycline resistance protein"
FT /id="PRO_0000173383"
FT TRANSMEM 12..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 324..344
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 346..365
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 432..451
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 458 AA; 49756 MW; 31D7B1256FD4B90E CRC64;
MNTSYSQSTL RHNQVLIWLC VLSFFSVLNE MVLNVSLPDI ANEFNKLPAS ANWVNTAFML
TFSIGTALYG KLSDQLGIKN LLLFGIMVNG LGSIIGFVGH SFFPILILAR FIQGIGAAAF
PALVMVVVAR YIPKENRGKA FGLIGSLVAM GEGVGPAIGG MVAHYIHWSY LLLIPTATII
TVPFLIKLLK KEERIRGHID MAGIILMSAG IVFFMLFTTS YRFSFLIISI LAFFIFVQHI
RKAQDPFVDP ELGKNVFFVI GTLCGGLIFG TVAGFVSMVP YMMKDVHHLS TAAIGSGIIF
PGTMSVIIFG YIGGLLVDRK GSLYVLTIGS ALLSSGFLIA AFFIDAAPWI MTIIVIFVFG
GLSFTKTVIS TVVSSSLKEK EAGAGMSLLN FTSFLSEGTG IAIVGGLLSI GFLDHRLLPI
DVDHSTYLYS NMLILFAGII VICWLVILNV YKRSRRHG
