TCRX_MYCTU
ID TCRX_MYCTU Reviewed; 234 AA.
AC O69730; L0TF65; Q50808;
DT 27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 3.
DT 25-MAY-2022, entry version 149.
DE RecName: Full=Probable transcriptional regulatory protein TcrX;
GN Name=tcrX; OrderedLocusNames=Rv3765c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 16-103.
RC STRAIN=ATCC 25618 / H37Rv;
RA Casali N., Wren B.W., Stoker N.G.;
RL Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, AND GENE NAME.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=12595424; DOI=10.1128/iai.71.3.1134-1140.2003;
RA Parish T., Smith D.A., Kendall S., Casali N., Bancroft G.J., Stoker N.G.;
RT "Deletion of two-component regulatory systems increases the virulence of
RT Mycobacterium tuberculosis.";
RL Infect. Immun. 71:1134-1140(2003).
RN [4]
RP FUNCTION, AND PHOSPHORYLATION.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=19962420; DOI=10.1016/j.biochi.2009.11.009;
RA Bhattacharya M., Biswas A., Das A.K.;
RT "Interaction analysis of TcrX/Y two component system from Mycobacterium
RT tuberculosis.";
RL Biochimie 92:263-272(2010).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Member of the two-component regulatory system TcrY/TcrX.
CC {ECO:0000269|PubMed:12595424, ECO:0000269|PubMed:19962420}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Phosphorylated by TcrY. {ECO:0000269|PubMed:19962420}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking TcrX and TcrY show an increase in
CC virulence in mouse model of infection, with significantly shorter
CC survival times. {ECO:0000269|PubMed:12595424}.
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DR EMBL; X99370; CAA67749.1; -; Genomic_DNA.
DR EMBL; AL123456; CCP46592.1; -; Genomic_DNA.
DR PIR; F70801; F70801.
DR RefSeq; NP_218282.1; NC_000962.3.
DR RefSeq; WP_003420552.1; NZ_NVQJ01000009.1.
DR AlphaFoldDB; O69730; -.
DR SMR; O69730; -.
DR STRING; 83332.Rv3765c; -.
DR PaxDb; O69730; -.
DR DNASU; 886100; -.
DR GeneID; 45427765; -.
DR GeneID; 886100; -.
DR KEGG; mtu:Rv3765c; -.
DR TubercuList; Rv3765c; -.
DR eggNOG; COG0745; Bacteria.
DR InParanoid; O69730; -.
DR OMA; QLWGYPP; -.
DR PhylomeDB; O69730; -.
DR SABIO-RK; O69730; -.
DR PHI-base; PHI:3615; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0010106; P:cellular response to iron ion starvation; IEP:MTBBASE.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..234
FT /note="Probable transcriptional regulatory protein TcrX"
FT /id="PRO_0000412201"
FT DOMAIN 10..124
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 135..232
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 59
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT CONFLICT 81
FT /note="L -> P (in Ref. 2; CAA67749)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 234 AA; 25844 MW; 4595223F8B0F9604 CRC64;
MRRADGQPVT VLVVDDEPVL AEMVSMALRY EGWNITTAGD GSSAIAAARR QRPDVVVLDV
MLPDMSGLDV LHKLRSENPG LPVLLLTAKD AVEDRIAGLT AGGDDYVTKP FSIEEVVLRL
RALLRRTGVT TVDSGAQLVV GDLVLDEDSH EVMRAGEPVS LTSTEFELLR FMMHNSKRVL
SKAQILDRVW SYDFGGRSNI VELYISYLRK KIDNGREPMI HTLRGAGYVL KPAR
