ID   TCR_BACIU               Reviewed;         458 AA.
AC   P0A4K8; P11063; P72219;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Tetracycline resistance protein;
GN   Name=tet;
OS   Bacillus subtilis.
OG   Plasmid pHY163PLK, Plasmid pNS1981, and Plasmid pTB19.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=pHY163PLK;
RX   PubMed=6186390; DOI=10.1016/0092-8674(83)90502-0;
RA   Selzer G., Som T., Itoh T., Tomizawa J.;
RT   "The origin of replication of plasmid P15A and comparative studies on the
RT   nucleotide sequences around the origin of related plasmids.";
RL   Cell 32:119-129(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=pHY163PLK;
RA   Ishiwa H., Shibahara H.;
RT   "New shuttle vectors for Escherichia coli and Bacillus subtilis. III.
RT   Nucleotide sequence analysis of tetracycline resistance gene of pAMalpha1
RT   and ori-177.";
RL   Jpn. J. Genet. 60:485-498(1985).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / VKM B-501; PLASMID=pNS1981;
RX   PubMed=3090576; DOI=10.1016/0147-619x(86)90081-8;
RA   Makaguchi R., Shishido K., Hoshino T., Furukawa K.;
RT   "The nucleotide sequence of the tetracycline resistance gene of plasmid
RT   pNS1981 from Bacillus subtilis differs from pTHT15 from a Thermophilic
RT   bacillus by two base pairs.";
RL   Plasmid 16:72-73(1986).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=pTB19;
RX   PubMed=1946749; DOI=10.1016/0147-619x(91)90034-t;
RA   Oskam L., Hillenga D.J., Venema G., Bron S.;
RT   "The large Bacillus plasmid pTB19 contains two integrated rolling-circle
RT   plasmids carrying mobilization functions.";
RL   Plasmid 26:30-39(1991).
CC   -!- FUNCTION: Resistance to tetracycline by an active tetracycline efflux.
CC       This is an energy-dependent process that decreases the accumulation of
CC       the antibiotic in whole cells. This protein functions as a metal-
CC       tetracycline/H(+) antiporter.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
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DR   EMBL; D13792; -; NOT_ANNOTATED_CDS; Other_DNA.
DR   EMBL; D00006; BAA00005.1; -; Genomic_DNA.
DR   EMBL; M63891; AAA98304.1; -; Genomic_DNA.
DR   RefSeq; WP_001574277.1; NZ_JYFL01000027.1.
DR   AlphaFoldDB; P0A4K8; -.
DR   SMR; P0A4K8; -.
DR   GeneID; 66465484; -.
DR   PATRIC; fig|1423.174.peg.4451; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Antiport; Cell membrane; Hydrogen ion transport;
KW   Ion transport; Membrane; Plasmid; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..458
FT                   /note="Tetracycline resistance protein"
FT                   /id="PRO_0000173391"
FT   TRANSMEM        12..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..100
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        140..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        165..185
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        201..221
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        223..240
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        256..276
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        297..317
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        324..344
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        346..365
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        432..451
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   458 AA;  50093 MW;  6A708777D44C2074 CRC64;
     MNTSYSQSNL RHNQILIWLC ILSFFSVLNE MVLNVSLPDI ANDFNKPPAS TNWVNTAFML
     TFSIGTAVYG KLSDQLGIKR LLLFGIIINC FGSVIGFVGH SFFSLLIMAR FIQGAGAAAF
     PALVMVVVAR YIPKENRGKA FGLIGSIVAM GEGVGPAIGG MIAHYIHWSY LLLIPMITII
     TVPFLMKLLK KEVRIKGHFD IKGIILMSVG IVFFMLFTTS YSISFLIVSV LSFLIFVKHI
     RKVTDPFVDP GLGKNIPFMI GVLCGGIIFG TVAGFVSMVP YMMKDVHQLS TAEIGSVIIF
     PGTMSVIIFG YIGGILVDRR GPLYVLNIGV TFLSVSFLTA SFLLETTSWF MTIIIVFVLG
     GLSFTKTVIS TIVSSSLKQQ EAGAGMSLLN FTSFLSEGTG IAIVGGLLSI PLLDQRLLPM
     EVDQSTYLYS NLLLLFSGII VISWLVTLNV YKHSQRDF