ID   TCR_GEOSE               Reviewed;         458 AA.
AC   P07561;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Tetracycline resistance protein;
GN   Name=tet;
OS   Geobacillus stearothermophilus (Bacillus stearothermophilus).
OG   Plasmid pTHT15.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2996983; DOI=10.1016/0378-1119(85)90265-3;
RA   Hoshino T., Ikeda T., Tomizuka N., Furukawa K.;
RT   "Nucleotide sequence of the tetracycline resistance gene of pTHT15, a
RT   thermophilic Bacillus plasmid: comparison with staphylococcal TcR
RT   controls.";
RL   Gene 37:131-138(1985).
CC   -!- FUNCTION: Resistance to tetracycline by an active tetracycline efflux.
CC       This is an energy-dependent process that decreases the accumulation of
CC       the antibiotic in whole cells. This protein functions as a metal-
CC       tetracycline/H(+) antiporter.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
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DR   EMBL; M11036; AAA22851.1; -; Genomic_DNA.
DR   RefSeq; WP_063856080.1; NG_048201.1.
DR   AlphaFoldDB; P07561; -.
DR   SMR; P07561; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Antiport; Cell membrane; Hydrogen ion transport;
KW   Ion transport; Membrane; Plasmid; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..458
FT                   /note="Tetracycline resistance protein"
FT                   /id="PRO_0000173382"
FT   TRANSMEM        12..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..100
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        140..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        165..185
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        201..221
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        223..240
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        256..276
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        297..317
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        324..344
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        346..365
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        432..451
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   458 AA;  50119 MW;  E1F33F4C57E7B06A CRC64;
     MNTSYSQSNL RHNQILIWLC ILSFFSVLNE MVLNVSLPDI ANDFNKPPAS TNWVNTAFML
     TFSIGTAVYG KLSDQLGIKR LLLFGIIINC FGSVIGFVGH SFFSLLIMAR FIQGAGAAAF
     PALVMVVVAR YIPKENRGKA FGLIGSIVAM GEGVGPAIGG MIAHYIHWSY LLLIPMITII
     TVPFLMKLLK KEVRIKGHFD IKGIILMSVG IVFFMLFTTS YSISFLIVSV LSFLIFVKHI
     RKVTDPFVDP GLGKNIPFMI GVLCGGIIFG TVAGFVSMVP YMMKDVHQLS TAEIGSVIIF
     PGTMSVIIFG YIGGILVDRR GPLYVLNIGV TFLSVSFLTA SFLLETTSWF MTIIIVFVLG
     GLLFTKTVIS TIVSSSLKQQ EAGAGMSLLN FTSFLSEGTG IAIVGGLLSI PLLDQRLLPM
     EVDQSTYLYS NLLLLFSGII VISWLVTLNV YKHSQRDF