TCR_STAAU
ID TCR_STAAU Reviewed; 459 AA.
AC P02983; P03863; P14512; Q79CG9;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 31-AUG-2004, sequence version 3.
DT 25-MAY-2022, entry version 133.
DE RecName: Full=Tetracycline resistance protein;
DE AltName: Full=Tetracycline efflux protein;
GN Name=tet; Synonyms=tet(K);
OS Staphylococcus aureus.
OG Plasmid pT181, Plasmid pTP5, Plasmid pKH6, and Plasmid pKH1.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=1280;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pT181;
RX PubMed=6657777; DOI=10.1016/0147-619x(83)90039-2;
RA Khan S.A., Novick R.P.;
RT "Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid
RT from Staphylococcus aureus.";
RL Plasmid 10:251-259(1983).
RN [2]
RP SEQUENCE REVISION.
RC PLASMID=pT181;
RX PubMed=3142848; DOI=10.1128/jb.170.12.5522-5528.1988;
RA Mojumdar M., Khan S.A.;
RT "Characterization of the tetracycline resistance gene of plasmid pT181 of
RT Staphylococcus aureus.";
RL J. Bacteriol. 170:5522-5528(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pTP5;
RA Noguchi N., Aoki T., Sasatsu M., Kono M., Shishido K., Ando T.;
RT "Determination of the complete nucleotide sequence of pNS1, a
RT staphylococcal tetracycline-resistance plasmid propagated in Bacillus
RT subtilis.";
RL FEMS Microbiol. Lett. 37:283-288(1986).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pKH6;
RA Moon K.H., Lee D.W., Yoon S.J., Kim W.K., Shin C.K., Im S.H.;
RT "Complete nucleotide sequence of pKH6, a tetracycline-resistance plasmid
RT from multidrug-resistant Staphylococcus aureus SA2.";
RL Sanop Misaengmul Hakhoe Chi 24:423-426(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pT181;
RX PubMed=8234490; DOI=10.1006/plas.1993.1045;
RA Guay G.G., Khan S.A., Rothstein D.M.;
RT "The tet(K) gene of plasmid pT181 of Staphylococcus aureus encodes an
RT efflux protein that contains 14 transmembrane helices.";
RL Plasmid 30:163-166(1993).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pKH1;
RA Moon K.H., Kim W.K., Yoon S.J., Kim J.M., Shin C.K., Im S.H.;
RT "Association of tet gene with partial sequence of IS431mec in tetracycline
RT resistance plasmid pKH1.";
RL Arch. Pharm. Res. 19:171-172(1996).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pKH1;
RA Moon K.H., Kim W.K., Yoon S.J., Kim J.M., Shin C.K., Im S.H.;
RT "Relationship between two tetracycline resistance plasmids of
RT Staphylococcus aureus in Korea.";
RL J. Microbiol. Biotechnol. 6:292-294(1996).
CC -!- FUNCTION: Resistance to tetracycline by an active tetracycline efflux.
CC This is an energy-dependent process that decreases the accumulation of
CC the antibiotic in whole cells. This protein functions as a metal-
CC tetracycline/H(+) antiporter.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA26034.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAA26035.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; J01764; AAA26034.1; ALT_SEQ; Genomic_DNA.
DR EMBL; J01764; AAA26035.1; ALT_INIT; Genomic_DNA.
DR EMBL; M23644; AAA98301.1; -; Genomic_DNA.
DR EMBL; M16217; AAA19179.1; -; Unassigned_DNA.
DR EMBL; U38428; AAB07712.1; -; Genomic_DNA.
DR EMBL; S67449; AAB28795.1; -; Genomic_DNA.
DR EMBL; U38656; AAB09660.1; -; Genomic_DNA.
DR PIR; A04492; QQSACT.
DR PIR; S42238; S42238.
DR RefSeq; NP_040465.1; NC_001391.1.
DR RefSeq; NP_040470.1; NC_001393.1.
DR RefSeq; NP_040471.1; NC_001393.1.
DR RefSeq; NP_044360.1; NC_001763.1.
DR RefSeq; NP_053795.1; NC_001767.1.
DR RefSeq; WP_000492283.1; NZ_WTUM01000069.1.
DR RefSeq; WP_010889938.1; NC_001763.1.
DR RefSeq; WP_010889939.1; NC_001393.1.
DR RefSeq; YP_001649337.1; NC_010262.1.
DR RefSeq; YP_006937482.1; NC_013307.1.
DR RefSeq; YP_006937490.1; NC_013309.1.
DR RefSeq; YP_006937494.1; NC_013311.1.
DR RefSeq; YP_006937498.1; NC_013312.1.
DR RefSeq; YP_006937647.1; NC_013320.1.
DR RefSeq; YP_006937988.1; NC_013328.1.
DR RefSeq; YP_006938259.1; NC_013336.1.
DR AlphaFoldDB; P02983; -.
DR SMR; P02983; -.
DR TCDB; 2.A.1.3.6; the major facilitator superfamily (mfs).
DR GeneID; 50019748; -.
DR GeneID; 61682012; -.
DR GeneID; 64117985; -.
DR OMA; ESRAMNH; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Antiport; Cell membrane; Hydrogen ion transport;
KW Ion transport; Membrane; Plasmid; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..459
FT /note="Tetracycline resistance protein"
FT /id="PRO_0000173384"
FT TRANSMEM 12..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..218
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..283
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 322..344
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 428..450
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 361
FT /note="G -> E (in Ref. 1; AAA26034)"
FT /evidence="ECO:0000305"
FT CONFLICT 438
FT /note="I -> N (in Ref. 1; AAA26034)"
FT /evidence="ECO:0000305"
FT CONFLICT 443
FT /note="C -> S (in Ref. 1; AAA26034)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 459 AA; 50695 MW; FE53C55535372B41 CRC64;
MFSLYKKFKG LFYSVLFWLC ILSFFSVLNE MVLNVSLPDI ANHFNTTPGI TNWVNTAYML
TFSIGTAVYG KLSDYINIKK LLIIGISLSC LGSLIAFIGH NHFFILIFGR LVQGVGSAAF
PSLIMVVVAR NITRKKQGKA FGFIGSIVAL GEGLGPSIGG IIAHYIHWSY LLILPMITIV
TIPFLIKVMV PGKSTKNTLD IVGIVLMSIS IICFMLFTTN YNWTFLILFT IFFVIFIKHI
SRVSNPFINP KLGKNIPFML GLFSGGLIFS IVAGFISMVP YMMKTIYHVN VATIGNSVIF
PGTMSVIVFG YFGGFLVDRK GSLFVFILGS LSISISFLTI AFFVEFSMWL TTFMFIFVMG
GLSFTKTVIS KIVSSSLSEE EVASGMSLLN FTSFLSEGTG IAIVGGLLSL QLINRKLVLE
FINYSSGVYS NILVAMAILI ILCCLLTIIV FKRSEKQFE
