ID   TCR_STRAG               Reviewed;         458 AA.
AC   P13924;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Tetracycline resistance protein;
GN   Name=tet;
OS   Streptococcus agalactiae.
OG   Plasmid pMV158.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=1311;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2677995; DOI=10.1093/nar/17.18.7283;
RA   van der Lelie D., Bron S., Venema G., Oskam L.;
RT   "Similarity of minus origins of replication and flanking open reading
RT   frames of plasmids pUB110, pTB913 and pMV158.";
RL   Nucleic Acids Res. 17:7283-7294(1989).
CC   -!- FUNCTION: Resistance to tetracycline by an active tetracycline efflux.
CC       This is an energy-dependent process that decreases the accumulation of
CC       the antibiotic in whole cells. This protein functions as a metal-
CC       tetracycline/H(+) antiporter.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
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DR   EMBL; X15669; CAA33712.1; -; Genomic_DNA.
DR   RefSeq; WP_012218461.1; NG_048207.1.
DR   RefSeq; YP_001586273.1; NC_010096.1.
DR   AlphaFoldDB; P13924; -.
DR   SMR; P13924; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Antiport; Cell membrane; Hydrogen ion transport;
KW   Ion transport; Membrane; Plasmid; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..458
FT                   /note="Tetracycline resistance protein"
FT                   /id="PRO_0000173386"
FT   TRANSMEM        12..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..100
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        140..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        165..185
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        201..221
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        223..240
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        256..276
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        297..317
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        324..344
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        346..365
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        432..451
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   458 AA;  50006 MW;  AD2014E7CA199995 CRC64;
     MNTSYSQSNL RHNQILIWLC ILSFFSVLNE MVLNVSLPDI ANDFNKPPAS TNWVNTAFML
     TFSIGTAVYG KLSDQLGIKR LLLFGIIINC FGSVIGFVGH SFFSLLIMAR FIQGAGAAAF
     PALVMVVVAR YIPKENRGKA FGLIGSIVAM GEGVGPAIVG MIAHYIHWSY LLLIPMITII
     TVPFLMKLLK KEVRIKGHFD IKGIILMSVG IVFFMLFTTS YSISFLIVSV LSFLIFVKHI
     RKVTDPFVDP GLGKNILFMI GVLCGGIIFG TVAGFVSMVP YMMKDVHQLS TAEIGSVIIF
     PGTMSVIIFG YIGGILVDRR GPLYVLNIGV TFLSVSFLTA FFLLETTSWF MTIIIVFVLG
     GLSFTKTVIP TIVSSSLKQQ EAGAGMSLLN FTSFLSEGTG IAIVGGLLSI PLLDQRLLPM
     GVDQSTYLYS NLLLLFSGII VISGLVTVNV YKHSQRDF