ID   TCR_STRRM               Reviewed;         347 AA.
AC   P14551;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Tetracycline resistance determinant;
DE   AltName: Full=TET347;
GN   Name=tetB; Synonyms=otrB;
OS   Streptomyces rimosus.
OG   Plasmid.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1927;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3053973; DOI=10.1099/00221287-134-3-585;
RA   Reynes J.-P., Calmels T., Drocourt D., Tiraby G.;
RT   "Cloning, expression in Escherichia coli and nucleotide sequence of a
RT   tetracycline-resistance gene from Streptomyces rimosus.";
RL   J. Gen. Microbiol. 134:585-598(1988).
CC   -!- FUNCTION: Resistance to tetracycline by an active tetracycline efflux.
CC       This is an energy-dependent process that decreases the accumulation of
CC       the antibiotic in whole cells. This protein functions as a metal-
CC       tetracycline/H(+) antiporter.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
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DR   EMBL; M20370; AAA26831.1; -; Genomic_DNA.
DR   PIR; A46567; A46567.
DR   AlphaFoldDB; P14551; -.
DR   SMR; P14551; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Antiport; Cell membrane; Hydrogen ion transport;
KW   Ion transport; Membrane; Plasmid; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..347
FT                   /note="Tetracycline resistance determinant"
FT                   /id="PRO_0000173388"
FT   TRANSMEM        3..20
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        30..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        73..95
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        108..130
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..156
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        161..183
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        204..226
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        294..316
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          321..347
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   347 AA;  35818 MW;  AC8EFB68F1A9299E CRC64;
     MDVLGAAALA LFLVPLLIVA EQGRTWGWGS PAALALFALG AAGLAVFIPV ELRRGDEAIL
     PLGLFRRGSI ALCSAVNFTI GVGIFGTVTT LPLFLQMVQG RTPTQAGLVV IPFMLGTIAS
     QMVSGKLIAS SGRFKKLAIV GLGSMAGALL AMATTGATTP MWGIVLIVLW LGVGIGLSQT
     VITSPMQNSA PKSQLGVANG ASACAGQIGG STGIAVLFSV MFAVALGRLA DLLHTPRYER
     LLTDPAITGD PANHRFLDMA ESGQGAGINL DDTSLLNGID ARLMQPVTDS FAHGFHIMFL
     PGGVVLLAGF VMTWFLRELQ EETAPEEERP AESGAGAKNG PLPASDA