ID   TCSR3_PAES9             Reviewed;         170 AA.
AC   T0CUB8;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2013, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=RNA polymerase sigma factor TcsR {ECO:0000305};
GN   Name=tcsR {ECO:0000303|PubMed:23873908};
GN   ORFNames=H477_0267 {ECO:0000312|EMBL:EPZ61155.1};
OS   Paeniclostridium sordellii (strain ATCC 9714 / DSM 2141 / JCM 3814 / LMG
OS   15708 / NCIMB 10717 / 211) (Clostridium sordellii).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC   Paeniclostridium.
OX   NCBI_TaxID=1292036;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], FUNCTION, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=ATCC 9714 / DSM 2141 / JCM 3814 / LMG 15708 / NCIMB 10717 / 211;
RX   PubMed=23873908; DOI=10.1128/jb.00711-13;
RA   Sirigi Reddy A.R., Girinathan B.P., Zapotocny R., Govind R.;
RT   "Identification and characterization of Clostridium sordellii toxin gene
RT   regulator.";
RL   J. Bacteriol. 195:4246-4254(2013).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released (Probable). Transcriptional regulator specifically required to
CC       activate expression of the toxin gene locus, composed of tcsL and
CC       tcdE/utxA (PubMed:23873908). {ECO:0000269|PubMed:23873908,
CC       ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking tcsR grow normally but display
CC       reduced toxin production. {ECO:0000269|PubMed:23873908}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR   EMBL; APWR01000088; EPZ61155.1; -; Genomic_DNA.
DR   AlphaFoldDB; T0CUB8; -.
DR   SMR; T0CUB8; -.
DR   EnsemblBacteria; EPZ61155; EPZ61155; H477_0267.
DR   PATRIC; fig|1292036.3.peg.1021; -.
DR   Proteomes; UP000015748; Unassembled WGS sequence.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..170
FT                   /note="RNA polymerase sigma factor TcsR"
FT                   /id="PRO_0000451205"
FT   DNA_BIND        143..162
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000305"
FT   REGION          122..169
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   170 AA;  20434 MW;  C5D3BAC14524B94A CRC64;
     MSNLYESIRK YKCGYIEEIL NILDMFDPLL NKFQRNSCYE DMKSELSLFM FNLIDNFPLE
     KDCFKEDKFI INYIYKSLKN KFIQVNKLHQ KVKSYETNID IIWVNNCDYA NLLSTVIFED
     IIKDLTQNEK NILRKIYLHG LRESEISREL NISRQAVNKT HLRALEKLKN