ID   TCYJ_BACSU              Reviewed;         269 AA.
AC   O34406; Q795U6;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=L-cystine-binding protein TcyJ;
DE   Flags: Precursor;
GN   Name=tcyJ; Synonyms=ytmJ; OrderedLocusNames=BSU29380;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9387221; DOI=10.1099/00221287-143-11-3431;
RA   Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT   "Sequencing and functional annotation of the Bacillus subtilis genes in the
RT   200 kb rrnB-dnaB region.";
RL   Microbiology 143:3431-3441(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   INDUCTION.
RC   STRAIN=168;
RX   PubMed=12193636; DOI=10.1128/jb.184.18.5179-5186.2002;
RA   Auger S., Danchin A., Martin-Verstraete I.;
RT   "Global expression profile of Bacillus subtilis grown in the presence of
RT   sulfate or methionine.";
RL   J. Bacteriol. 184:5179-5186(2002).
RN   [4]
RP   FUNCTION IN L-CYSTINE TRANSPORT.
RC   STRAIN=168;
RX   PubMed=15262924; DOI=10.1128/jb.186.15.4875-4884.2004;
RA   Burguiere P., Auger S., Hullo M.-F., Danchin A., Martin-Verstraete I.;
RT   "Three different systems participate in L-cystine uptake in Bacillus
RT   subtilis.";
RL   J. Bacteriol. 186:4875-4884(2004).
CC   -!- FUNCTION: Part of the ABC transporter complex TcyJKLMN involved in L-
CC       cystine import. Is also involved in cystathionine, djenkolate, and S-
CC       methylcysteine transport. {ECO:0000269|PubMed:15262924}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (TcyN),
CC       two transmembrane proteins (TcyL and TcyM) and two solute-binding
CC       proteins (TcyJ and TcyK). {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}.
CC   -!- INDUCTION: More strongly expressed in the presence of methionine than
CC       in the presence of sulfate. {ECO:0000269|PubMed:12193636}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein 3 family.
CC       {ECO:0000305}.
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DR   EMBL; AF008220; AAC00325.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14898.1; -; Genomic_DNA.
DR   PIR; D69996; D69996.
DR   RefSeq; NP_390816.1; NC_000964.3.
DR   RefSeq; WP_004399114.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; O34406; -.
DR   SMR; O34406; -.
DR   STRING; 224308.BSU29380; -.
DR   TCDB; 3.A.1.3.13; the atp-binding cassette (abc) superfamily.
DR   PaxDb; O34406; -.
DR   PRIDE; O34406; -.
DR   EnsemblBacteria; CAB14898; CAB14898; BSU_29380.
DR   GeneID; 937354; -.
DR   KEGG; bsu:BSU29380; -.
DR   PATRIC; fig|224308.179.peg.3192; -.
DR   eggNOG; COG0834; Bacteria.
DR   InParanoid; O34406; -.
DR   OMA; QNQLDGF; -.
DR   PhylomeDB; O34406; -.
DR   BioCyc; BSUB:BSU29380-MON; -.
DR   SABIO-RK; O34406; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   InterPro; IPR001638; Solute-binding_3/MltF_N.
DR   Pfam; PF00497; SBP_bac_3; 1.
DR   SMART; SM00062; PBPb; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Amino-acid transport; Cell membrane; Lipoprotein; Membrane; Palmitate;
KW   Reference proteome; Signal; Transport.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           21..269
FT                   /note="L-cystine-binding protein TcyJ"
FT                   /id="PRO_0000281739"
FT   LIPID           21
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           21
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   269 AA;  30174 MW;  9563896705018458 CRC64;
     MNKRKGLVLL LSVFALLGGG CSQTNNKTDR QAQTVIVGTG TDFPNIAFLN EKGELTGYDI
     EVMKAIDKEL PQYTFEFKTM DFSNLLTSLG NKKIDVIAHN MAKNKEREKR FLYHKVPYNY
     SPMYITVKED NHKIHTLKDL HGKTVIVGAT SNAADYITKY NKTHGSPIHL KYAGQGSNDT
     ANQIETGRAD ATIATPFAVD FQNKTHAFRQ KTVGDVLLDT EVYFMFNKGS QTLADDTDQA
     IKKLEKNGTL KKLSRKWLGA DYSKSSFEK