TDA2_YEAST
ID TDA2_YEAST Reviewed; 126 AA.
AC P40045; D3DLX6;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Topoisomerase I damage affected protein 2;
GN Name=TDA2; OrderedLocusNames=YER071C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=19053807; DOI=10.1021/pr800524g;
RA Narayanaswamy R., Moradi E.K., Niu W., Hart G.T., Davis M., McGary K.L.,
RA Ellington A.D., Marcotte E.M.;
RT "Systematic definition of protein constituents along the major polarization
RT axis reveals an adaptive reuse of the polarization machinery in pheromone-
RT treated budding yeast.";
RL J. Proteome Res. 8:6-19(2009).
RN [7]
RP DISRUPTION PHENOTYPE.
RX PubMed=21173034; DOI=10.1101/gr.109033.110;
RA Reid R.J., Gonzalez-Barrera S., Sunjevaric I., Alvaro D., Ciccone S.,
RA Wagner M., Rothstein R.;
RT "Selective ploidy ablation, a high-throughput plasmid transfer protocol,
RT identifies new genes affecting topoisomerase I-induced DNA damage.";
RL Genome Res. 21:477-486(2011).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Cell
CC projection {ECO:0000269|PubMed:19053807}. Note=Concentrates at
CC cytoplasmic punctate structures and localizes at the mating projection
CC tip. {ECO:0000269|PubMed:19053807}.
CC -!- DISRUPTION PHENOTYPE: Leads to cell death when overexpressing the
CC camptothecin mimetic TOP1-T(722)A mutant.
CC {ECO:0000269|PubMed:21173034}.
CC -!- MISCELLANEOUS: Present with 799 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the TDA2 family. {ECO:0000305}.
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DR EMBL; U18813; AAB64607.1; -; Genomic_DNA.
DR EMBL; AY692721; AAT92740.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07730.1; -; Genomic_DNA.
DR PIR; S50574; S50574.
DR RefSeq; NP_010994.3; NM_001178962.3.
DR PDB; 5VKY; X-ray; 2.30 A; A/B=2-126.
DR PDBsum; 5VKY; -.
DR AlphaFoldDB; P40045; -.
DR SMR; P40045; -.
DR BioGRID; 36814; 80.
DR ComplexPortal; CPX-3541; AIM21-TDA2 actin assembly regulator complex.
DR DIP; DIP-4134N; -.
DR IntAct; P40045; 5.
DR MINT; P40045; -.
DR STRING; 4932.YER071C; -.
DR iPTMnet; P40045; -.
DR MaxQB; P40045; -.
DR PaxDb; P40045; -.
DR PRIDE; P40045; -.
DR EnsemblFungi; YER071C_mRNA; YER071C; YER071C.
DR GeneID; 856802; -.
DR KEGG; sce:YER071C; -.
DR SGD; S000000873; TDA2.
DR VEuPathDB; FungiDB:YER071C; -.
DR eggNOG; ENOG502S7YH; Eukaryota.
DR HOGENOM; CLU_137494_1_0_1; -.
DR InParanoid; P40045; -.
DR OMA; ITIIWIS; -.
DR BioCyc; YEAST:G3O-30243-MON; -.
DR PRO; PR:P40045; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P40045; protein.
DR GO; GO:0030479; C:actin cortical patch; IDA:SGD.
DR GO; GO:0110131; C:Aim21-Tda2 complex; IPI:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0043332; C:mating projection tip; HDA:SGD.
DR GO; GO:0030837; P:negative regulation of actin filament polymerization; IPI:SGD.
DR GO; GO:2000813; P:negative regulation of barbed-end actin filament capping; IDA:ComplexPortal.
DR Gene3D; 3.30.1140.40; -; 1.
DR InterPro; IPR005334; Tctex-1-like.
DR InterPro; IPR038586; Tctex-1-like_sf.
DR Pfam; PF03645; Tctex-1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cell projection; Cytoplasm; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..126
FT /note="Topoisomerase I damage affected protein 2"
FT /id="PRO_0000202634"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22814378"
SQ SEQUENCE 126 AA; 14498 MW; 2F5CD27AF0C9AFE3 CRC64;
MSMQIEIKDG RSDNSPLPER KLVTLIQESY DSLKDDNEIN LSTESTSNLL IKLVLEKLEK
HSSLYKYIAS VTTLNIEGLN EENANFSLKN DIGASWESKK DGIFNYKLED KNNNECYLIT
ILWLHK
