TDA6_YEAST
ID TDA6_YEAST Reviewed; 467 AA.
AC Q06466; D6W4F3;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Putative vacuolar protein sorting-associated protein TDA6;
DE AltName: Full=Topoisomerase I damage affected protein 6;
GN Name=TDA6; OrderedLocusNames=YPR157W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP INDUCTION.
RX PubMed=17608707; DOI=10.1111/j.1567-1364.2007.00270.x;
RA Dardalhon M., Lin W., Nicolas A., Averbeck D.;
RT "Specific transcriptional responses induced by 8-methoxypsoralen and UVA in
RT yeast.";
RL FEMS Yeast Res. 7:866-878(2007).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=21173034; DOI=10.1101/gr.109033.110;
RA Reid R.J., Gonzalez-Barrera S., Sunjevaric I., Alvaro D., Ciccone S.,
RA Wagner M., Rothstein R.;
RT "Selective ploidy ablation, a high-throughput plasmid transfer protocol,
RT identifies new genes affecting topoisomerase I-induced DNA damage.";
RL Genome Res. 21:477-486(2011).
CC -!- FUNCTION: Involved in vacuolar protein sorting. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein.
CC -!- INDUCTION: By 8-methoxypsoralen and UVA. {ECO:0000269|PubMed:17608707}.
CC -!- DISRUPTION PHENOTYPE: Leads to cell death when overexpressing the
CC camptothecin mimetic TOP1-T(722)A mutant.
CC {ECO:0000269|PubMed:21173034}.
CC -!- SIMILARITY: Belongs to the VPS62 family. {ECO:0000305}.
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DR EMBL; U28371; AAB68054.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11569.1; -; Genomic_DNA.
DR PIR; S61141; S61141.
DR RefSeq; NP_015483.1; NM_001184254.1.
DR AlphaFoldDB; Q06466; -.
DR BioGRID; 36324; 42.
DR DIP; DIP-3824N; -.
DR MINT; Q06466; -.
DR STRING; 4932.YPR157W; -.
DR PaxDb; Q06466; -.
DR PRIDE; Q06466; -.
DR EnsemblFungi; YPR157W_mRNA; YPR157W; YPR157W.
DR GeneID; 856280; -.
DR KEGG; sce:YPR157W; -.
DR SGD; S000006361; TDA6.
DR VEuPathDB; FungiDB:YPR157W; -.
DR eggNOG; ENOG502RJPB; Eukaryota.
DR GeneTree; ENSGT00940000176347; -.
DR HOGENOM; CLU_024079_2_0_1; -.
DR InParanoid; Q06466; -.
DR OMA; GRGTHAN; -.
DR BioCyc; YEAST:G3O-34288-MON; -.
DR PRO; PR:Q06466; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q06466; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
PE 2: Evidence at transcript level;
KW Glycoprotein; Membrane; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..467
FT /note="Putative vacuolar protein sorting-associated protein
FT TDA6"
FT /id="PRO_0000259482"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 61
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 124
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 141
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 467 AA; 53809 MW; 163CBC858653B60D CRC64;
MHCVLARILL WFLIVDLSVI RALVLPPLKD YDPLEPLMKR DMAMGQRNRF KVDGQLPPIL
NSTDVTDDQR SLHTPGEIPS YVINHCPLVH LYSEEKYWPS DIAEYVQNFQ IKDKNGNSIS
THENLTLHDL KAEYHVDLFG NKTETHIPSS EVFLTSLDDF DKDPKWLLGH LPEYGTGYNS
KAPAILIVVD KGNGWVDAFW FFFYPFNHGP FIMGHGPWGN HVGDWEHSLV RFYKGIPKYL
WMSAHSSGTG YRYEAVEKFK KLRKRKQQDS DDGGDTILER PLIFSARGTH ANYASAGQHA
HDIPFFFMPL SDFTDRGPLW DPSLNFYSYT FDGKTVTPSS EREESLGLDW LHFQGGWGDQ
QLPARDPRQK WCVAQWKYIG GPRGPLFKKL DRLNLCGGVK KWNFWNGGCP ARRLIKKAEG
LDSESTDLMG DNCGVLLYRI RPKWLRGILR FLMWRGILCS LMEFFTN
