ID   TDEA_AGGAC              Reviewed;         457 AA.
AC   Q2EHL7;
DT   07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Toxin and drug export protein A {ECO:0000303|PubMed:17116373};
DE   Flags: Precursor;
GN   Name=tdeA {ECO:0000303|PubMed:17116373};
OS   Aggregatibacter actinomycetemcomitans (Actinobacillus
OS   actinomycetemcomitans) (Haemophilus actinomycetemcomitans).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Aggregatibacter.
OX   NCBI_TaxID=714;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=IDH781;
RX   PubMed=17116373; DOI=10.1016/j.gene.2006.10.004;
RA   Crosby J.A., Kachlany S.C.;
RT   "TdeA, a TolC-like protein required for toxin and drug export in
RT   Aggregatibacter (Actinobacillus) actinomycetemcomitans.";
RL   Gene 388:83-92(2007).
RN   [2]
RP   SUBUNIT, AND CHARACTERIZATION OF THE PERIPLASMIC DOMAIN.
RX   PubMed=18633280;
RA   Kim S., Yum S., Jo W.S., Lee B.L., Jeong M.H., Ha N.C.;
RT   "Expression and biochemical characterization of the periplasmic domain of
RT   bacterial outer membrane porin TdeA.";
RL   J. Microbiol. Biotechnol. 18:845-851(2008).
CC   -!- FUNCTION: Required for secretion of the LtxA leukotoxin and resistance
CC       to various antimicrobial compounds. {ECO:0000269|PubMed:17116373}.
CC   -!- SUBUNIT: Homotrimer (PubMed:18633280). Probably part of a complex
CC       composed of LtxB, LtxD and TdeA, which forms a single transport channel
CC       across the two membranes (PubMed:17116373).
CC       {ECO:0000269|PubMed:18633280, ECO:0000303|PubMed:17116373}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane
CC       {ECO:0000250|UniProtKB:P77211}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:P77211}.
CC   -!- DOMAIN: The periplasmic domain interacts in vitro with purified
CC       peptidoglycans. {ECO:0000269|PubMed:18633280}.
CC   -!- DISRUPTION PHENOTYPE: Mutant cannot secrete leukotoxin and is more
CC       sensitive to clotrimazole, erythromycin, ethidium bromide, cationic
CC       detergents, bile-acids, anionic detergents and chloramphenicol.
CC       {ECO:0000269|PubMed:17116373}.
CC   -!- SIMILARITY: Belongs to the outer membrane factor (OMF) (TC 1.B.17)
CC       family. {ECO:0000305}.
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DR   EMBL; DQ378166; ABD38133.1; -; Genomic_DNA.
DR   RefSeq; WP_005545419.1; NZ_VSEW01000002.1.
DR   AlphaFoldDB; Q2EHL7; -.
DR   SMR; Q2EHL7; -.
DR   STRING; 714.ACT75_10870; -.
DR   TCDB; 1.B.17.3.11; the outer membrane factor (omf) family.
DR   eggNOG; COG1538; Bacteria.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0015288; F:porin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR003423; OMP_efflux.
DR   InterPro; IPR010131; RND_efflux_OM_lipoprot_NodT.
DR   Pfam; PF02321; OEP; 2.
DR   TIGRFAMs; TIGR01845; outer_NodT; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Cell outer membrane; Ion transport; Membrane; Porin;
KW   Signal; Transmembrane; Transmembrane beta strand; Transport.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..457
FT                   /note="Toxin and drug export protein A"
FT                   /id="PRO_0000431390"
SQ   SEQUENCE   457 AA;  51118 MW;  F4AE6BEB1F8510BA CRC64;
     MFTIKKLTLT IVVATTLTGC ANIGDSYRAS LKNYKQYEEI TKQYNIKNDW WKLYKDAQLN
     RVVEKALLNN KDLAKATISV NRALYSANLA GANLVPAFSG STRSTAQKNI KTGGNSTISH
     TGSLNVSYTL DLWFRLADTA DAAEWAHKAT VQDMESTKLS LINSVVTTYY QIAYLNDAIS
     TTKESIKYYT DISNIMRNRL AQGVADSISV DQAQQAVLTA RNNLITYQLN RKTAEQTLRN
     LLNLKPDETL KITFPHILKV KSVGVNLNVP VSVIANRPDI KGYQARLSSA FKNVKATEKS
     WFPEITLGGS LNSSGKKLNS ATNTLIGGGA LGISLPFLNW NTVKWNVKIS EADYETARLN
     YEKSITVALN DVDTNYFSFT QAKKRFTNAQ KTYIYNQRIT QYYRNRYNAG VSELREWLTA
     ANTEKNSQLS ILQAKYNVIQ AENAVYSSMA GYYSVKK