BR2EF_PELRI
ID BR2EF_PELRI Reviewed; 33 AA.
AC P86153;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 20-JAN-2009, sequence version 1.
DT 25-MAY-2022, entry version 23.
DE RecName: Full=Brevinin-2Ef {ECO:0000303|PubMed:18855342};
OS Pelophylax ridibundus (Marsh frog) (Rana ridibunda).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Pelophylax.
OX NCBI_TaxID=8406;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, MASS SPECTROMETRY, AND DISULFIDE BOND.
RC TISSUE=Skin secretion {ECO:0000303|PubMed:18855342};
RX PubMed=18855342; DOI=10.1002/rcm.3759;
RA Samgina T.Y., Artemenko K.A., Gorshkov V.A., Ogourtsov S.V., Zubarev R.A.,
RA Lebedev A.T.;
RT "De novo sequencing of peptides secreted by the skin glands of the
RT caucasian green frog Rana ridibunda.";
RL Rapid Commun. Mass Spectrom. 22:3517-3525(2008).
RN [2]
RP PROTEIN SEQUENCE, SUBCELLULAR LOCATION, DISULFIDE BOND, MASS SPECTROMETRY,
RP AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Skin secretion {ECO:0000303|PubMed:28012108};
RX PubMed=28012108; DOI=10.1007/s00216-016-0143-3;
RA Samgina T.Y., Artemenko K.A., Bergquist J., Trebse P., Torkar G.,
RA Tolpina M.D., Lebedev A.T.;
RT "Differentiation of frogs from two populations belonging to the Pelophylax
RT esculentus complex by LC-MS/MS comparison of their skin peptidomes.";
RL Anal. Bioanal. Chem. 409:1951-1961(2017).
CC -!- FUNCTION: Shows antibacterial activity against representative Gram-
CC negative and Gram-positive bacterial species, and hemolytic activity.
CC {ECO:0000250|UniProtKB:P40842}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18855342,
CC ECO:0000269|PubMed:28012108}.
CC -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC {ECO:0000305|PubMed:28012108}.
CC -!- MASS SPECTROMETRY: Mass=3369; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:18855342};
CC -!- MASS SPECTROMETRY: Mass=3362.8; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:28012108};
CC -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC Brevinin subfamily. {ECO:0000255}.
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DR AlphaFoldDB; P86153; -.
DR SMR; P86153; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR InterPro; IPR012521; Antimicrobial_frog_2.
DR Pfam; PF08023; Antimicrobial_2; 1.
PE 1: Evidence at protein level;
KW Amphibian defense peptide; Antibiotic; Antimicrobial; Cytolysis;
KW Direct protein sequencing; Disulfide bond; Hemolysis; Secreted.
FT PEPTIDE 1..33
FT /note="Brevinin-2Ef"
FT /evidence="ECO:0000269|PubMed:18855342,
FT ECO:0000269|PubMed:28012108"
FT /id="PRO_0000361060"
FT DISULFID 27..33
FT /evidence="ECO:0000269|PubMed:18855342,
FT ECO:0000269|PubMed:28012108"
SQ SEQUENCE 33 AA; 3367 MW; 9913918BB2ABB0EE CRC64;
GIMDTLKNLA KTAGKGALQS LVKMASCKLS GQC