BR2GC_SYLGU
ID BR2GC_SYLGU Reviewed; 72 AA.
AC A0AEI6;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2006, sequence version 1.
DT 03-AUG-2022, entry version 31.
DE RecName: Full=Brevinin-2GHc;
DE AltName: Full=AMP-4;
DE Flags: Precursor;
GN Name=br2GHc {ECO:0000312|EMBL:CAK18910.1};
OS Sylvirana guentheri (Gunther's frog) (Rana guentheri).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Sylvirana.
OX NCBI_TaxID=110109;
RN [1] {ECO:0000305, ECO:0000312|EMBL:CAK18910.1}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 42-72, FUNCTION,
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISULFIDE BOND, AND MASS
RP SPECTROMETRY.
RC TISSUE=Skin {ECO:0000269|PubMed:16979798}, and
RC Skin secretion {ECO:0000269|PubMed:16979798};
RX PubMed=16979798; DOI=10.1016/j.peptides.2006.08.007;
RA Zhou J., McClean S., Thompson A., Zhang Y., Shaw C., Rao P., Bjourson A.J.;
RT "Purification and characterization of novel antimicrobial peptides from the
RT skin secretion of Hylarana guentheri.";
RL Peptides 27:3077-3084(2006).
CC -!- FUNCTION: Antimicrobial peptide. Active against the Gram-positive
CC bacteria S.aureus FDA209P (MIC=9.8 ug/ml) and B.subtilis ATCC 6633
CC (MIC>64 ug/ml), and the Gram-negative bacteria E.coli O111 (MIC=19.6
CC ug/ml) and E.coli ATCC 25922 (MIC=9.8 ug/ml). Not active against the
CC fungus C.albicans. {ECO:0000269|PubMed:16979798}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16979798}.
CC -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC {ECO:0000269|PubMed:16979798}.
CC -!- MASS SPECTROMETRY: Mass=3258.1; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:16979798};
CC -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC Brevinin subfamily. {ECO:0000255}.
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DR EMBL; AM262990; CAK18910.1; -; mRNA.
DR AlphaFoldDB; A0AEI6; -.
DR SMR; A0AEI6; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProt.
DR InterPro; IPR012521; Antimicrobial_frog_2.
DR InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR Pfam; PF08023; Antimicrobial_2; 1.
DR Pfam; PF03032; FSAP_sig_propep; 1.
PE 1: Evidence at protein level;
KW Amphibian defense peptide; Antibiotic; Antimicrobial;
KW Direct protein sequencing; Disulfide bond; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..42
FT /evidence="ECO:0000255, ECO:0000269|PubMed:16979798"
FT /id="PRO_0000271187"
FT PEPTIDE 43..72
FT /note="Brevinin-2GHc"
FT /evidence="ECO:0000269|PubMed:16979798"
FT /id="PRO_5000147966"
FT DISULFID 64..70
FT /evidence="ECO:0000269|PubMed:16979798"
SQ SEQUENCE 72 AA; 8008 MW; 996940A92B644E05 CRC64;
MFTMKKSLLL LFFLGMISLS LCEQERGADE DEGEVEEQIK RSIWEGIKNA GKGFLVSILD
KVRCKVAGGC NP