ID   TDM1A_MISBR             Reviewed;          42 AA.
AC   P83608;
DT   27-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   27-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Delta-actinopoditoxin-Mb1a;
DE            Short=Delta-AOTX-Mb1a;
DE   AltName: Full=Delta-missulenatoxin-Mb1a;
DE            Short=Delta-MSTX-Mb1a;
OS   Missulena bradleyi (Eastern mouse spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Actinopodidae; Missulena.
OX   NCBI_TaxID=230234;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP   MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=14596942; DOI=10.1016/s0014-5793(03)01175-x;
RA   Gunning S.J., Chong Y., Khalife A.A., Hains P.G., Broady K.W.,
RA   Nicholson G.M.;
RT   "Isolation of delta-missulenatoxin-Mb1a, the major vertebrate-active spider
RT   delta-toxin from the venom of Missulena bradleyi (Actinopodidae).";
RL   FEBS Lett. 554:211-218(2003).
CC   -!- FUNCTION: Lethal neurotoxin active on both vertebrates and insects.
CC       Slows the inactivation of vertebrate tetrodotoxin-sensitive voltage-
CC       gated sodium channels (Nav) and most likely insect sodium channels
CC       presumably by binding to site 3 of the channel. Effects are an increase
CC       in resting tension, a muscle fasciculation and a decrease in indirect
CC       twitch tension. It fails to affect tetrodotoxin-resistant sodium
CC       currents. {ECO:0000269|PubMed:14596942}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:14596942}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:14596942}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: Mass=4933; Mass_error=1; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:14596942};
CC   -!- MISCELLANEOUS: In this species, the venom of the male is lethal rather
CC       than that of the female.
CC   -!- MISCELLANEOUS: Is the sole lethal toxin in the venom of male
CC       M.bradleyi.
CC   -!- SIMILARITY: Belongs to the neurotoxin 06 (delta-actx) family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P83608; -.
DR   SMR; P83608; -.
DR   ArachnoServer; AS000169; delta-actinopoditoxin-Mb1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR008017; Atracotoxin_delta.
DR   Pfam; PF05353; Atracotoxin; 1.
DR   PROSITE; PS60018; DELTA_ACTX; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..42
FT                   /note="Delta-actinopoditoxin-Mb1a"
FT                   /id="PRO_0000087656"
FT   DISULFID        1..15
FT                   /evidence="ECO:0000250"
FT   DISULFID        8..20
FT                   /evidence="ECO:0000250"
FT   DISULFID        14..31
FT                   /evidence="ECO:0000250"
FT   DISULFID        16..42
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   42 AA;  4942 MW;  4FB2EF873B4458C7 CRC64;
     CAKKREWCAK TEDCCCPMKC IYAWYNEQSS CQTTFSGMFK KC