TDT_ARATH
ID TDT_ARATH Reviewed; 540 AA.
AC Q8LG88; Q9FEH9; Q9FGK1; Q9MAW4;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Tonoplast dicarboxylate transporter;
DE Short=AttDT;
DE AltName: Full=Sodium-dicarboxylate cotransporter-like;
DE Short=AtSDAT;
DE AltName: Full=Vacuolar malate transporter;
GN Name=TDT; Synonyms=SDAT; OrderedLocusNames=At5g47560; ORFNames=MNJ7.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND INDUCTION.
RX PubMed=12947042; DOI=10.1073/pnas.1832002100;
RA Emmerlich V., Linka N., Reinhold T., Hurth M.A., Traub M., Martinoia E.,
RA Neuhaus H.E.;
RT "The plant homolog to the human sodium/dicarboxylic cotransporter is the
RT vacuolar malate carrier.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:11122-11126(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Saito K., Takahashi H.;
RT "Sodium sulfate or dicarboxylate transporter form Arabidopsis thaliana.";
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION, AND INDUCTION.
RX PubMed=15728336; DOI=10.1104/pp.104.058453;
RA Hurth M.A., Suh S.J., Kretzschmar T., Geis T., Bregante M., Gambale F.,
RA Martinoia E., Neuhaus H.E.;
RT "Impaired pH homeostasis in Arabidopsis lacking the vacuolar dicarboxylate
RT transporter and analysis of carboxylic acid transport across the
RT tonoplast.";
RL Plant Physiol. 137:901-910(2005).
CC -!- FUNCTION: Putative carrier protein indirectly involved in the uptake of
CC malate and fumarate to the vacuole, probably by regulating the
CC energization across the tonoplast. Uptake of malate to vacuoles is
CC inhibited by citrate and by the uncoupler carbonyl-cyanide m-
CC chlorophenylhydrazone, but seems to be not affected by sodium. Critical
CC for pH homeostasis. {ECO:0000269|PubMed:12947042,
CC ECO:0000269|PubMed:15728336}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:12947042};
CC Multi-pass membrane protein {ECO:0000269|PubMed:12947042}.
CC Note=Tonoplast.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, stems and flowers.
CC {ECO:0000269|PubMed:12947042}.
CC -!- INDUCTION: Induced by malate and by acidification of the cytoplasm.
CC {ECO:0000269|PubMed:12947042, ECO:0000269|PubMed:15728336}.
CC -!- SIMILARITY: Belongs to the SLC13A/DASS transporter (TC 2.A.47) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB09082.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAC19843.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AJ223445; CAC19843.1; ALT_FRAME; mRNA.
DR EMBL; AB043024; BAA96091.1; -; mRNA.
DR EMBL; AB025628; BAB09082.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95532.1; -; Genomic_DNA.
DR EMBL; AY072145; AAL59967.1; -; mRNA.
DR EMBL; AY133792; AAM91726.1; -; mRNA.
DR EMBL; AY084408; AAM60982.1; -; mRNA.
DR RefSeq; NP_199567.1; NM_124129.4.
DR AlphaFoldDB; Q8LG88; -.
DR SMR; Q8LG88; -.
DR BioGRID; 20054; 2.
DR IntAct; Q8LG88; 1.
DR STRING; 3702.AT5G47560.1; -.
DR TCDB; 2.A.47.1.6; the divalent anion:na(+) symporter (dass) family.
DR PaxDb; Q8LG88; -.
DR PRIDE; Q8LG88; -.
DR ProteomicsDB; 246434; -.
DR EnsemblPlants; AT5G47560.1; AT5G47560.1; AT5G47560.
DR GeneID; 834806; -.
DR Gramene; AT5G47560.1; AT5G47560.1; AT5G47560.
DR KEGG; ath:AT5G47560; -.
DR Araport; AT5G47560; -.
DR TAIR; locus:2168988; AT5G47560.
DR eggNOG; KOG1281; Eukaryota.
DR HOGENOM; CLU_005170_9_0_1; -.
DR InParanoid; Q8LG88; -.
DR OMA; LHGMNTY; -.
DR OrthoDB; 389981at2759; -.
DR PhylomeDB; Q8LG88; -.
DR PRO; PR:Q8LG88; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8LG88; baseline and differential.
DR Genevisible; Q8LG88; AT.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005773; C:vacuole; IDA:TAIR.
DR GO; GO:0015140; F:malate transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0098656; P:anion transmembrane transport; IBA:GO_Central.
DR GO; GO:0015743; P:malate transport; TAS:TAIR.
DR GO; GO:0051453; P:regulation of intracellular pH; TAS:TAIR.
DR InterPro; IPR001898; SLC13A/DASS.
DR Pfam; PF00939; Na_sulph_symp; 1.
DR TIGRFAMs; TIGR00785; dass; 1.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport; Vacuole.
FT CHAIN 1..540
FT /note="Tonoplast dicarboxylate transporter"
FT /id="PRO_0000172526"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 419..439
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 466..486
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 520..540
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 310
FT /note="D -> E (in Ref. 6; AAM60982)"
FT /evidence="ECO:0000305"
FT CONFLICT 407
FT /note="R -> P (in Ref. 1; CAC19843)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 540 AA; 58098 MW; 4BF0107A9C4B3B74 CRC64;
MNGGDVTVAG SDDLKSPLLP VVHNDEPFER QTVGQQLRTI FTPKNCYIAL GPLLCAVVCL
CVDLGGDETT TARNMLGVLV WMFAWWLTEA VPMPITSMTP LFLFPLFGIS AADDVANSYM
DDVISLVLGS FILALAVEHY NIHRRLALNI TLVFCVEPLN APLLLLGICA TTAFVSMWMH
NVAAAVMMMP VATGILQRLP SSSSTTEVVH PAVGKFSRAV VLGVIYSAAV GGMSTLTGTG
VNLILVGMWK SYFPEADPIS FSQWFFFGFP LALCIFVVLW CVLCVMYCPK GAGQALSPYL
HKSHLRRELD LLGPMNFAEK MVLAVFGGLV VLWMTRNITD DIPGWGRIFA GRAGDGTVSV
MMATLLFIIP SNIKKGEKLM DWNKCKKLPW NIVLLLGAGF AIADGVRTSG LAEVLSKGLV
FLETAPYWAI APTVCLIAAT ITEFTSNNAT TTLLVPLLIE IAKNMGIHPL LLMVPGAIGA
QFAFLLPTGT PSNVVGFTTG HIEIKDMIKT GLPLKIAGTI FLSILMPTLG AYVFASMGGV
