ID   BR2RK_PELRI             Reviewed;          33 AA.
AC   C0HL04;
DT   20-DEC-2017, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2017, sequence version 1.
DT   25-MAY-2022, entry version 6.
DE   RecName: Full=Brevinin-2Rk {ECO:0000303|PubMed:28012108};
OS   Pelophylax ridibundus (Marsh frog) (Rana ridibunda).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Pelophylax.
OX   NCBI_TaxID=8406;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Skin secretion {ECO:0000303|PubMed:28012108};
RX   PubMed=28012108; DOI=10.1007/s00216-016-0143-3;
RA   Samgina T.Y., Artemenko K.A., Bergquist J., Trebse P., Torkar G.,
RA   Tolpina M.D., Lebedev A.T.;
RT   "Differentiation of frogs from two populations belonging to the Pelophylax
RT   esculentus complex by LC-MS/MS comparison of their skin peptidomes.";
RL   Anal. Bioanal. Chem. 409:1951-1961(2017).
CC   -!- FUNCTION: Antimicrobial peptide. {ECO:0000250|UniProtKB:P0C5X2}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28012108}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:28012108}.
CC   -!- MASS SPECTROMETRY: Mass=3504.9; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:28012108};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; C0HL04; -.
DR   SMR; C0HL04; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0006952; P:defense response; IEA:InterPro.
DR   InterPro; IPR012521; Antimicrobial_frog_2.
DR   Pfam; PF08023; Antimicrobial_2; 1.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Disulfide bond; Secreted.
FT   PEPTIDE         1..33
FT                   /note="Brevinin-2Rk"
FT                   /evidence="ECO:0000269|PubMed:28012108"
FT                   /id="PRO_0000442764"
FT   DISULFID        27..33
FT                   /evidence="ECO:0000269|PubMed:28012108"
SQ   SEQUENCE   33 AA;  3509 MW;  A590DA4AED928631 CRC64;
     GLWKTLKDSA KSAVTNVAVT MLDKLRCKLT GGC