TEAD2_MOUSE
ID TEAD2_MOUSE Reviewed; 445 AA.
AC P48301; Q62297;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Transcriptional enhancer factor TEF-4;
DE AltName: Full=ETEF-1;
DE AltName: Full=Embryonic TEA domain-containing factor;
DE Short=ETF;
DE AltName: Full=TEA domain family member 2;
DE Short=TEAD-2;
GN Name=Tead2; Synonyms=Etdf, Etf, Tef4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=ICR; TISSUE=Head;
RX PubMed=7629195; DOI=10.1074/jbc.270.31.18649;
RA Yasunami M., Suzuki K., Houtani T., Sugimoto T., Ohkubo H.;
RT "Molecular characterization of cDNA encoding a novel protein related to
RT transcriptional enhancer factor-1 from neural precursor cells.";
RL J. Biol. Chem. 270:18649-18654(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=8812452; DOI=10.1006/geno.1996.0461;
RA Suzuki K., Yasunami M., Matsuda Y., Maeda T., Kobayashi H., Terasaki H.,
RA Ohkubo H.;
RT "Structural organization and chromosomal assignment of the mouse embryonic
RT TEA domain-containing factor (ETF) gene.";
RL Genomics 36:263-270(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8702974; DOI=10.1074/jbc.271.36.21775;
RA Jacquemin P., Hwang J.-J., Martial J.A., Dolle P., Davidson I.;
RT "A novel family of developmentally regulated mammalian transcription
RT factors containing the TEA/ATTS DNA binding domain.";
RL J. Biol. Chem. 271:21775-21785(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Carcinoma;
RX PubMed=9169843; DOI=10.1242/dev.124.10.1963;
RA Kaneko K.J., Cullinan E.B., Latham K.E., Depamphilis M.L.;
RT "Transcription factor mTEAD-2 is selectively expressed at the beginning of
RT zygotic gene expression in the mouse.";
RL Development 124:1963-1973(1997).
CC -!- FUNCTION: Transcription factor which plays a key role in the Hippo
CC signaling pathway, a pathway involved in organ size control and tumor
CC suppression by restricting proliferation and promoting apoptosis. The
CC core of this pathway is composed of a kinase cascade wherein MST1/MST2,
CC in complex with its regulatory protein SAV1, phosphorylates and
CC activates LATS1/2 in complex with its regulatory protein MOB1, which in
CC turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ.
CC Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby
CC regulating cell proliferation, migration and epithelial mesenchymal
CC transition (EMT) induction (By similarity). Binds to the SPH and GT-IIC
CC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation
CC of neural development. Binds to the M-CAT motif. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with YAP1 and WWTR1/TAZ. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Highest expression in brain. High levels also found
CC in lung, testis and ovarian follicle cells. Lower levels in heart and
CC spleen.
CC -!- DEVELOPMENTAL STAGE: In the embryo, levels fall after fertilization
CC until the 2-4 cell stage and then increase rapidly.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D50563; BAA09126.1; -; mRNA.
DR EMBL; D83596; BAA12018.1; -; Genomic_DNA.
DR EMBL; X94442; CAA64216.1; -; mRNA.
DR EMBL; Y10026; CAA71135.1; -; mRNA.
DR CCDS; CCDS21236.1; -.
DR PIR; A57400; A57400.
DR RefSeq; NP_001272427.1; NM_001285498.1.
DR RefSeq; NP_035695.1; NM_011565.2.
DR RefSeq; XP_006540840.1; XM_006540777.2.
DR AlphaFoldDB; P48301; -.
DR SMR; P48301; -.
DR BioGRID; 204099; 3.
DR CORUM; P48301; -.
DR IntAct; P48301; 1.
DR STRING; 10090.ENSMUSP00000103431; -.
DR iPTMnet; P48301; -.
DR PhosphoSitePlus; P48301; -.
DR PaxDb; P48301; -.
DR PRIDE; P48301; -.
DR ProteomicsDB; 259369; -.
DR Antibodypedia; 31984; 263 antibodies from 33 providers.
DR DNASU; 21677; -.
DR Ensembl; ENSMUST00000033060; ENSMUSP00000033060; ENSMUSG00000030796.
DR Ensembl; ENSMUST00000107801; ENSMUSP00000103431; ENSMUSG00000030796.
DR GeneID; 21677; -.
DR KEGG; mmu:21677; -.
DR UCSC; uc009gud.1; mouse.
DR CTD; 8463; -.
DR MGI; MGI:104904; Tead2.
DR VEuPathDB; HostDB:ENSMUSG00000030796; -.
DR eggNOG; KOG3841; Eukaryota.
DR GeneTree; ENSGT00950000182956; -.
DR HOGENOM; CLU_012515_2_0_1; -.
DR InParanoid; P48301; -.
DR OMA; WQTRALG; -.
DR OrthoDB; 823827at2759; -.
DR PhylomeDB; P48301; -.
DR TreeFam; TF313443; -.
DR Reactome; R-MMU-2032785; YAP1- and WWTR1 (TAZ)-stimulated gene expression.
DR Reactome; R-MMU-8951671; RUNX3 regulates YAP1-mediated transcription.
DR BioGRID-ORCS; 21677; 6 hits in 74 CRISPR screens.
DR ChiTaRS; Tead2; mouse.
DR PRO; PR:P48301; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; P48301; protein.
DR Bgee; ENSMUSG00000030796; Expressed in ventricular zone and 197 other tissues.
DR ExpressionAtlas; P48301; baseline and differential.
DR Genevisible; P48301; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0140552; C:TEAD-YAP complex; ISO:MGI.
DR GO; GO:0005667; C:transcription regulator complex; IDA:MGI.
DR GO; GO:0097718; F:disordered domain specific binding; ISO:MGI.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0001223; F:transcription coactivator binding; ISO:MGI.
DR GO; GO:0071300; P:cellular response to retinoic acid; IDA:MGI.
DR GO; GO:0003143; P:embryonic heart tube morphogenesis; IGI:MGI.
DR GO; GO:0048568; P:embryonic organ development; IBA:GO_Central.
DR GO; GO:0035329; P:hippo signaling; ISO:MGI.
DR GO; GO:0048368; P:lateral mesoderm development; IGI:MGI.
DR GO; GO:0060548; P:negative regulation of cell death; IGI:UniProtKB.
DR GO; GO:0001843; P:neural tube closure; IMP:MGI.
DR GO; GO:0030903; P:notochord development; IGI:MGI.
DR GO; GO:0048339; P:paraxial mesoderm development; IGI:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0065003; P:protein-containing complex assembly; ISO:MGI.
DR GO; GO:2000736; P:regulation of stem cell differentiation; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0001570; P:vasculogenesis; IGI:MGI.
DR Gene3D; 6.10.20.40; -; 1.
DR InterPro; IPR000818; TEA/ATTS_dom.
DR InterPro; IPR038096; TEA/ATTS_sf.
DR InterPro; IPR016361; TEF_metazoa.
DR InterPro; IPR041086; YBD.
DR Pfam; PF01285; TEA; 1.
DR Pfam; PF17725; YBD; 1.
DR PIRSF; PIRSF002603; TEF; 1.
DR PRINTS; PR00065; TEADOMAIN.
DR SMART; SM00426; TEA; 1.
DR PROSITE; PS00554; TEA_1; 1.
DR PROSITE; PS51088; TEA_2; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..445
FT /note="Transcriptional enhancer factor TEF-4"
FT /id="PRO_0000205933"
FT DNA_BIND 38..114
FT /note="TEA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00505"
FT REGION 1..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 172..445
FT /note="Transcriptional activation"
FT /evidence="ECO:0000255"
FT REGION 191..217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 195..217
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 289
FT /note="Y -> F (in Ref. 3; CAA64216)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 445 AA; 49041 MW; 237BC27A8BF9877C CRC64;
MGDPRTGAPL DDGGGWTGSE EGSEEGTGGS EGVGGDGSPD AEGVWSPDIE QSFQEALAIY
PPCGRRKIIL SDEGKMYGRN ELIARYIKLR TGKTRTRKQV SSHIQVLARR KSREIQSKLK
DQVSKDKAFQ TMATMSSAQL ISAPSLQAKL GPSGPQATEL FQFWSGSSGP PWNVPDVKPF
SQAPFSVSLT PPASDLPGYE PPPALSPLPP PAPSPPAWQA RALGTARLQL IEFSAFVEPP
DAVDSFQRHL FVHISQQCPS PGAPPLESVD VRQIYDKFPE KKGGLRELYD RGPPHAFFLV
KFWADLNWGP SAEEAGSSGG GGGFYGVSSQ YESRELMTLT CSSKVCSFGK QVVEKVETER
AQLEDGRFVY RLLRSPMCEY LVNFLHKLRQ LPERYMMNSV LENFTILQVV TNRDTQELLL
CTAYVFEVST SERGAQYHIY RLVRD
