ID   BR2TB_RANTE             Reviewed;          74 AA.
AC   P82269;
DT   04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Brevinin-2Tb;
DE   Flags: Precursor;
OS   Rana temporaria (European common frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Rana; Rana.
OX   NCBI_TaxID=8407;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 42-74.
RC   TISSUE=Skin, and Skin secretion;
RX   PubMed=10333736;
RX   DOI=10.1002/(sici)1097-0282(1998)47:6<435::aid-bip3>3.0.co;2-8;
RA   Simmaco M., Mignogna G., Barra D.;
RT   "Antimicrobial peptides from amphibian skin: what do they tell us?";
RL   Biopolymers 47:435-450(1998).
CC   -!- FUNCTION: Antimicrobial peptide. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000305}.
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DR   EMBL; AJ251566; CAB61445.1; -; mRNA.
DR   AlphaFoldDB; P82269; -.
DR   SMR; P82269; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IEA:UniProt.
DR   InterPro; IPR012521; Antimicrobial_frog_2.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   Pfam; PF08023; Antimicrobial_2; 1.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..40
FT                   /id="PRO_0000003451"
FT   PEPTIDE         42..74
FT                   /note="Brevinin-2Tb"
FT                   /id="PRO_0000003452"
FT   DISULFID        68..74
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   74 AA;  8193 MW;  5B26718D62B79387 CRC64;
     MFTMKKSLLL FFFLGTISLS LCQEERNADE DDGEMTEEEK RGILDTLKHL AKTAGKGALQ
     SLLNHASCKL SGQC