TEC1_CANAL
ID TEC1_CANAL Reviewed; 743 AA.
AC Q5ANJ4; A0A1D8PJY3;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Transcription activator TEC1;
GN Name=TEC1; OrderedLocusNames=CAALFM_C304530CA;
GN ORFNames=CaO19.13329, CaO19.5908;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11069668; DOI=10.1046/j.1365-2958.2000.02132.x;
RA Schweizer A., Rupp S., Taylor B.N., Rollinghoff M., Schroppel K.;
RT "The TEA/ATTS transcription factor CaTec1p regulates hyphal development and
RT virulence in Candida albicans.";
RL Mol. Microbiol. 38:435-445(2000).
RN [5]
RP INDUCTION.
RX PubMed=11595734; DOI=10.1074/jbc.m104484200;
RA Lane S., Birse C., Zhou S., Matson R., Liu H.;
RT "DNA array studies demonstrate convergent regulation of virulence factors
RT by Cph1, Cph2, and Efg1 in Candida albicans.";
RL J. Biol. Chem. 276:48988-48996(2001).
RN [6]
RP FUNCTION, AND INDUCTION.
RX PubMed=11533231; DOI=10.1128/mcb.21.19.6418-6428.2001;
RA Lane S., Zhou S., Pan T., Dai Q., Liu H.;
RT "The basic helix-loop-helix transcription factor Cph2 regulates hyphal
RT development in Candida albicans partly via TEC1.";
RL Mol. Cell. Biol. 21:6418-6428(2001).
RN [7]
RP FUNCTION.
RX PubMed=15039365; DOI=10.1128/iai.72.4.2386-2389.2004;
RA Staib P., Binder A., Kretschmar M., Nichterlein T., Schroppel K.,
RA Morschhauser J.;
RT "Tec1p-independent activation of a hypha-associated Candida albicans
RT virulence gene during infection.";
RL Infect. Immun. 72:2386-2389(2004).
RN [8]
RP FUNCTION.
RX PubMed=16321041; DOI=10.1021/bp050236c;
RA Li F., Palecek S.P.;
RT "Identification of Candida albicans genes that induce Saccharomyces
RT cerevisiae cell adhesion and morphogenesis.";
RL Biotechnol. Prog. 21:1601-1609(2005).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15964282; DOI=10.1016/j.cub.2005.05.047;
RA Nobile C.J., Mitchell A.P.;
RT "Regulation of cell-surface genes and biofilm formation by the C. albicans
RT transcription factor Bcr1p.";
RL Curr. Biol. 15:1150-1155(2005).
RN [10]
RP FUNCTION.
RX PubMed=15755921; DOI=10.1128/ec.4.3.588-603.2005;
RA Bassilana M., Hopkins J., Arkowitz R.A.;
RT "Regulation of the Cdc42/Cdc24 GTPase module during Candida albicans hyphal
RT growth.";
RL Eukaryot. Cell 4:588-603(2005).
RN [11]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15819622; DOI=10.1111/j.1365-2958.2005.04576.x;
RA Biswas K., Morschhauser J.;
RT "The Mep2p ammonium permease controls nitrogen starvation-induced
RT filamentous growth in Candida albicans.";
RL Mol. Microbiol. 56:649-669(2005).
RN [12]
RP INDUCTION.
RX PubMed=16710830; DOI=10.1002/yea.1373;
RA Umeyama T., Kaneko A., Niimi M., Uehara Y.;
RT "Repression of CDC28 reduces the expression of the morphology-related
RT transcription factors, Efg1p, Nrg1p, Rbf1p, Rim101p, Fkh2p and Tec1p and
RT induces cell elongation in Candida albicans.";
RL Yeast 23:537-552(2006).
RN [13]
RP FUNCTION.
RX PubMed=17277173; DOI=10.1128/ec.00340-06;
RA Argimon S., Wishart J.A., Leng R., Macaskill S., Mavor A., Alexandris T.,
RA Nicholls S., Knight A.W., Enjalbert B., Walmsley R., Odds F.C., Gow N.A.,
RA Brown A.J.;
RT "Developmental regulation of an adhesin gene during cellular morphogenesis
RT in the fungal pathogen Candida albicans.";
RL Eukaryot. Cell 6:682-692(2007).
RN [14]
RP FUNCTION.
RX PubMed=17257864; DOI=10.1016/j.fgb.2006.12.006;
RA Eckert S.E., Heinz W.J., Zakikhany K., Thewes S., Haynes K., Hube B.,
RA Muhlschlegel F.A.;
RT "PGA4, a GAS homologue from Candida albicans, is up-regulated early in
RT infection processes.";
RL Fungal Genet. Biol. 44:368-377(2007).
RN [15]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19346360; DOI=10.1128/aem.00098-09;
RA Stichternoth C., Ernst J.F.;
RT "Hypoxic adaptation by Efg1 regulates biofilm formation by Candida
RT albicans.";
RL Appl. Environ. Microbiol. 75:3663-3672(2009).
RN [16]
RP INDUCTION.
RX PubMed=19197361; DOI=10.1371/journal.ppat.1000294;
RA Bastidas R.J., Heitman J., Cardenas M.E.;
RT "The protein kinase Tor1 regulates adhesin gene expression in Candida
RT albicans.";
RL PLoS Pathog. 5:E1000294-E1000294(2009).
RN [17]
RP FUNCTION.
RX PubMed=20435697; DOI=10.1128/ec.00034-10;
RA Rosenbach A., Dignard D., Pierce J.V., Whiteway M., Kumamoto C.A.;
RT "Adaptations of Candida albicans for growth in the mammalian intestinal
RT tract.";
RL Eukaryot. Cell 9:1075-1086(2010).
RN [18]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20223293; DOI=10.1016/j.micinf.2010.03.001;
RA Fuchs B.B., Eby J., Nobile C.J., El Khoury J.B., Mitchell A.P.,
RA Mylonakis E.;
RT "Role of filamentation in Galleria mellonella killing by Candida
RT albicans.";
RL Microbes Infect. 12:488-496(2010).
RN [19]
RP INDUCTION.
RX PubMed=20150241; DOI=10.1099/mic.0.037549-0;
RA Holcombe L.J., McAlester G., Munro C.A., Enjalbert B., Brown A.J.,
RA Gow N.A., Ding C., Butler G., O'Gara F., Morrissey J.P.;
RT "Pseudomonas aeruginosa secreted factors impair biofilm development in
RT Candida albicans.";
RL Microbiology 156:1476-1486(2010).
RN [20]
RP FUNCTION, AND INDUCTION.
RX PubMed=20454615; DOI=10.1371/journal.pbio.1000363;
RA Sahni N., Yi S., Daniels K.J., Huang G., Srikantha T., Soll D.R.;
RT "Tec1 mediates the pheromone response of the white phenotype of Candida
RT albicans: insights into the evolution of new signal transduction
RT pathways.";
RL PLoS Biol. 8:E1000363-E1000363(2010).
RN [21]
RP INDUCTION.
RX PubMed=21357478; DOI=10.1128/ec.00305-10;
RA Shareck J., Nantel A., Belhumeur P.;
RT "Conjugated linoleic acid inhibits hyphal growth in Candida albicans by
RT modulating Ras1p cellular levels and downregulating TEC1 expression.";
RL Eukaryot. Cell 10:565-577(2011).
RN [22]
RP INDUCTION.
RX PubMed=21498643; DOI=10.1128/ec.00014-11;
RA Kim Y., Mylonakis E.;
RT "Killing of Candida albicans filaments by Salmonella enterica serovar
RT Typhimurium is mediated by sopB effectors, parts of a type III secretion
RT system.";
RL Eukaryot. Cell 10:782-790(2011).
RN [23]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21407800; DOI=10.1371/journal.pone.0017046;
RA Wachtler B., Wilson D., Haedicke K., Dalle F., Hube B.;
RT "From attachment to damage: defined genes of Candida albicans mediate
RT adhesion, invasion and damage during interaction with oral epithelial
RT cells.";
RL PLoS ONE 6:E17046-E17046(2011).
RN [24]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22265407; DOI=10.1016/j.cell.2011.10.048;
RA Nobile C.J., Fox E.P., Nett J.E., Sorrells T.R., Mitrovich Q.M.,
RA Hernday A.D., Tuch B.B., Andes D.R., Johnson A.D.;
RT "A recently evolved transcriptional network controls biofilm development in
RT Candida albicans.";
RL Cell 148:126-138(2012).
RN [25]
RP INDUCTION.
RX PubMed=22544909; DOI=10.1128/ec.00103-12;
RA Fanning S., Xu W., Solis N., Woolford C.A., Filler S.G., Mitchell A.P.;
RT "Divergent targets of Candida albicans biofilm regulator Bcr1 in vitro and
RT in vivo.";
RL Eukaryot. Cell 11:896-904(2012).
RN [26]
RP FUNCTION.
RX PubMed=22923045; DOI=10.1128/ec.00217-12;
RA Daniels K.J., Pujol C., Srikantha T., Soll D.R.;
RT "The 'finger,' a unique multicellular morphology of Candida albicans
RT induced by CO2 and dependent upon the Ras1-cyclic AMP pathway.";
RL Eukaryot. Cell 11:1257-1267(2012).
RN [27]
RP INDUCTION.
RX PubMed=23236295; DOI=10.1371/journal.pgen.1003118;
RA Hnisz D., Bardet A.F., Nobile C.J., Petryshyn A., Glaser W., Schock U.,
RA Stark A., Kuchler K.;
RT "A histone deacetylase adjusts transcription kinetics at coding sequences
RT during Candida albicans morphogenesis.";
RL PLoS Genet. 8:E1003118-E1003118(2012).
RN [28]
RP FUNCTION.
RX PubMed=22276126; DOI=10.1371/journal.pone.0029707;
RA Du H., Guan G., Xie J., Sun Y., Tong Y., Zhang L., Huang G.;
RT "Roles of Candida albicans Gat2, a GATA-type zinc finger transcription
RT factor, in biofilm formation, filamentous growth and virulence.";
RL PLoS ONE 7:E29707-E29707(2012).
RN [29]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=23117819; DOI=10.4161/trns.22281;
RA Fox E.P., Nobile C.J.;
RT "A sticky situation: untangling the transcriptional network controlling
RT biofilm development in Candida albicans.";
RL Transcription 3:315-322(2012).
RN [30]
RP INDUCTION.
RX PubMed=23766273; DOI=10.1111/jicd.12048;
RA Bandara H.M., Cheung B.P., Watt R.M., Jin L.J., Samaranayake L.P.;
RT "Secretory products of Escherichia coli biofilm modulate Candida biofilm
RT formation and hyphal development.";
RL J. Investig. Clin. Dent. 4:186-199(2013).
RN [31]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23808664; DOI=10.1111/mmi.12310;
RA Guan G., Xie J., Tao L., Nobile C.J., Sun Y., Cao C., Tong Y., Huang G.;
RT "Bcr1 plays a central role in the regulation of opaque cell filamentation
RT in Candida albicans.";
RL Mol. Microbiol. 89:732-750(2013).
RN [32]
RP INDUCTION.
RX PubMed=24260276; DOI=10.1371/journal.pone.0079671;
RA Zhao L.X., Li D.D., Hu D.D., Hu G.H., Yan L., Wang Y., Jiang Y.Y.;
RT "Effect of tetrandrine against Candida albicans biofilms.";
RL PLoS ONE 8:E79671-E79671(2013).
RN [33]
RP FUNCTION.
RX PubMed=23637598; DOI=10.1371/journal.ppat.1003305;
RA Lin C.H., Kabrawala S., Fox E.P., Nobile C.J., Johnson A.D., Bennett R.J.;
RT "Genetic control of conventional and pheromone-stimulated biofilm formation
RT in Candida albicans.";
RL PLoS Pathog. 9:E1003305-E1003305(2013).
RN [34]
RP INDUCTION.
RX PubMed=23966855; DOI=10.1371/journal.ppat.1003519;
RA Znaidi S., Nesseir A., Chauvel M., Rossignol T., d'Enfert C.;
RT "A comprehensive functional portrait of two heat shock factor-type
RT transcriptional regulators involved in Candida albicans morphogenesis and
RT virulence.";
RL PLoS Pathog. 9:E1003519-E1003519(2013).
CC -!- FUNCTION: Transcription factor which regulates genes involved in hyphal
CC development, cell adhesion, biofilm development, and virulence. Plays a
CC role in the formation of 'finger' morphology, a unique multicellular
CC morphology of C.albicans induced by carbon dioxide. Regulates gene
CC expression during intestinal colonization. Required for the expression
CC of the secreted aspartyl proteinases SAP4, SAP5, and SAP6; but also of
CC BCR1, PGA4, and CDC24. Moreover, a positive feedback loop between CDC24
CC and TEC1 contributes to an increase in active CDC42 at the tip of the
CC germ tube which is important for hyphae formation. Regulates also the
CC pheromone response of the white cell phenotype.
CC {ECO:0000269|PubMed:11069668, ECO:0000269|PubMed:11533231,
CC ECO:0000269|PubMed:15039365, ECO:0000269|PubMed:15755921,
CC ECO:0000269|PubMed:15819622, ECO:0000269|PubMed:15964282,
CC ECO:0000269|PubMed:16321041, ECO:0000269|PubMed:17257864,
CC ECO:0000269|PubMed:17277173, ECO:0000269|PubMed:19346360,
CC ECO:0000269|PubMed:20223293, ECO:0000269|PubMed:20435697,
CC ECO:0000269|PubMed:20454615, ECO:0000269|PubMed:21407800,
CC ECO:0000269|PubMed:22265407, ECO:0000269|PubMed:22276126,
CC ECO:0000269|PubMed:22923045, ECO:0000269|PubMed:23117819,
CC ECO:0000269|PubMed:23637598, ECO:0000269|PubMed:23808664}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- INDUCTION: Expression is induced by CPH2 which directly binds to the
CC two sterol regulatory element 1-like elements upstream of TEC1.
CC Expression is also regulated by the histone deacetylase complex HDAC3,
CC as well as by TOR1 and EFG1. Moreover, heat shock factor-type
CC transcriptional regulator SFL1 represses expression whereas SFL2
CC induces expression. In white cells, is up-regulated in the presence of
CC alpha-pheromone. Expression is induced during oropharyngeal candidiasis
CC (OPC). Competitors Escherichia coli, Pseudomonas aeruginosa, and
CC Salmonella typhimurium secreted factors decrease TEC1 expression to
CC impair biofilm formation. Expression is also down-regulated by linoleic
CC acid and tetrandrine. {ECO:0000269|PubMed:11533231,
CC ECO:0000269|PubMed:11595734, ECO:0000269|PubMed:16710830,
CC ECO:0000269|PubMed:19197361, ECO:0000269|PubMed:20150241,
CC ECO:0000269|PubMed:20454615, ECO:0000269|PubMed:21357478,
CC ECO:0000269|PubMed:21498643, ECO:0000269|PubMed:22544909,
CC ECO:0000269|PubMed:23236295, ECO:0000269|PubMed:23766273,
CC ECO:0000269|PubMed:23966855, ECO:0000269|PubMed:24260276}.
CC -!- DISRUPTION PHENOTYPE: Impairs the formation of germ tubes and true
CC hyphae; and decreases virulence in a mouse model of systemic
CC candidiasis. Leads to defective biofilm under normoxic conditions but
CC not under hypoxic conditions. {ECO:0000269|PubMed:11069668,
CC ECO:0000269|PubMed:15819622, ECO:0000269|PubMed:15964282,
CC ECO:0000269|PubMed:19346360, ECO:0000269|PubMed:20223293,
CC ECO:0000269|PubMed:21407800, ECO:0000269|PubMed:22265407,
CC ECO:0000269|PubMed:23808664}.
CC -!- SIMILARITY: Belongs to the TEC1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP017625; AOW28471.1; -; Genomic_DNA.
DR RefSeq; XP_723122.1; XM_718029.1.
DR AlphaFoldDB; Q5ANJ4; -.
DR SMR; Q5ANJ4; -.
DR BioGRID; 1218355; 1.
DR STRING; 237561.Q5ANJ4; -.
DR PRIDE; Q5ANJ4; -.
DR GeneID; 3635261; -.
DR KEGG; cal:CAALFM_C304530CA; -.
DR CGD; CAL0000175954; TEC1.
DR VEuPathDB; FungiDB:C3_04530C_A; -.
DR eggNOG; KOG3841; Eukaryota.
DR HOGENOM; CLU_022080_0_0_1; -.
DR InParanoid; Q5ANJ4; -.
DR OMA; GKFRTRK; -.
DR OrthoDB; 645783at2759; -.
DR PHI-base; PHI:190; -.
DR PRO; PR:Q5ANJ4; -.
DR Proteomes; UP000000559; Chromosome 3.
DR GO; GO:0000785; C:chromatin; IDA:CGD.
DR GO; GO:0005634; C:nucleus; IDA:CGD.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:CGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:CGD.
DR GO; GO:0044406; P:adhesion of symbiont to host; IGI:CGD.
DR GO; GO:0007155; P:cell adhesion; IGI:CGD.
DR GO; GO:0030447; P:filamentous growth; IMP:CGD.
DR GO; GO:0044182; P:filamentous growth of a population of unicellular organisms; IMP:CGD.
DR GO; GO:0036180; P:filamentous growth of a population of unicellular organisms in response to biotic stimulus; IMP:CGD.
DR GO; GO:1900430; P:positive regulation of filamentous growth of a population of unicellular organisms; IMP:CGD.
DR GO; GO:1900445; P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus; IMP:CGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:CGD.
DR GO; GO:1900231; P:regulation of single-species biofilm formation on inanimate substrate; IMP:CGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:CGD.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:CGD.
DR GO; GO:0044011; P:single-species biofilm formation on inanimate substrate; IMP:CGD.
DR Gene3D; 6.10.20.40; -; 1.
DR InterPro; IPR000818; TEA/ATTS_dom.
DR InterPro; IPR038096; TEA/ATTS_sf.
DR Pfam; PF01285; TEA; 1.
DR PRINTS; PR00065; TEADOMAIN.
DR SMART; SM00426; TEA; 1.
DR PROSITE; PS00554; TEA_1; 1.
DR PROSITE; PS51088; TEA_2; 1.
PE 1: Evidence at protein level;
KW Cell adhesion; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Virulence.
FT CHAIN 1..743
FT /note="Transcription activator TEC1"
FT /id="PRO_0000425602"
FT DNA_BIND 178..252
FT /note="TEA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00505"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 104..165
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 629..657
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..165
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 743 AA; 83080 MW; A2B4AECA308705F1 CRC64;
MMSQATPSAT PVRNADGQKK GKKLPLIVDV AIGNNGKQLY QVHESSKMVR KEMPRSTNFA
LNDDISDEGF FISQINEQRT PIRKTLGTLS PSSLNQKRIR HDVYDQGDDN SNDDNCQNDV
YEQQQQQPQQ QQQQQQQLHQ PNMYENGNVG VISNDPVSSV GHYDNNPVSH VPTGAYSRVQ
DTDIWSDDVE EAFEEVLRLI PKSGLNKIKI AGRSCGRNEL ISDYIFAKTG KFRTRKQVSS
HIQVIKNLGQ KLDIIQLIND GPIFNSHEEQ LESTKKFEDV FSKINLNKSL GFSDSMKRKS
DSMPMHLPAT KRIRRKHSGN PLNKIKFSNF FMSVNDQYGM NPIVLTIQQN GNDVKSLKLK
DNANISSRFP GLSDFKSCPH IPIIHNMVKI LLPQLPESYS IDDGFSSSYA LKYEEPENAS
PTHTSIISSS RTYSLFTCVY SYGKEIVKFD EDGIQLNQDR EFIPGFWKFF FSTFGDQSEG
GLSAAFKGVT IKQILYESSP DSVKKEQDAS KVNKSKVKLV LLWEFAKVSE CKDALTTTTK
LVLPPRASAS SSKTTEEVFE YSEPALNSIG GTPTDTTSPN MDLNNQNLSA AATSIPGIRD
TIHSASMPDI NELPSSAKPQ VRLQKTFQSM QHLQPHQMWQ QQQQQQPSQG AYTSSVASQS
LNTSLSSPYA QYGMPLPQQT IGTFVPPTSQ TFGVSYTHNS QHPSANMDLM MLSSMNTGYG
NITNNQDYQF GNIGYTEGFT SEF
