TECB_CUPXP
ID TECB_CUPXP Reviewed; 275 AA.
AC O69264;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Chlorobenzene dihydrodiol dehydrogenase {ECO:0000303|PubMed:11526005};
DE EC=1.3.1.119 {ECO:0000269|PubMed:11526005, ECO:0000269|PubMed:15774876};
GN Name=tecB {ECO:0000303|PubMed:9249026};
OS Cupriavidus sp. (strain PS12).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Cupriavidus; unclassified Cupriavidus.
OX NCBI_TaxID=393999;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PS12;
RX PubMed=9249026; DOI=10.1111/j.1432-1033.1997.00190.x;
RA Beil S., Happe B., Timmis K.N., Pieper D.H.;
RT "Genetic and biochemical characterization of the broad spectrum
RT chlorobenzene dioxygenase from Burkholderia sp. strain PS12--dechlorination
RT of 1,2,4,5-tetrachlorobenzene.";
RL Eur. J. Biochem. 247:190-199(1997).
RN [2]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RC STRAIN=PS12;
RX PubMed=11526005; DOI=10.1128/aem.67.9.4057-4063.2001;
RA Pollmann K., Beil S., Pieper D.H.;
RT "Transformation of chlorinated benzenes and toluenes by Ralstonia sp.
RT strain PS12 tecA (tetrachlorobenzene dioxygenase) and tecB (chlorobenzene
RT dihydrodiol dehydrogenase) gene products.";
RL Appl. Environ. Microbiol. 67:4057-4063(2001).
RN [3]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RC STRAIN=PS12;
RX PubMed=15774876; DOI=10.1128/jb.187.7.2332-2340.2005;
RA Pollmann K., Wray V., Pieper D.H.;
RT "Chloromethylmuconolactones as critical metabolites in the degradation of
RT chloromethylcatechols: recalcitrance of 2-chlorotoluene.";
RL J. Bacteriol. 187:2332-2340(2005).
CC -!- FUNCTION: Can transform various dihydrodiols of chlorobenzenes and
CC chlorotoluenes into the respective catechols.
CC {ECO:0000269|PubMed:11526005, ECO:0000269|PubMed:15774876}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(1R,2R)-3-chlorocyclohexa-3,5-diene-1,2-diol + NAD(+) = 3-
CC chlorocatechol + H(+) + NADH; Xref=Rhea:RHEA:57608,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:19981, ChEBI:CHEBI:27715,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.3.1.119;
CC Evidence={ECO:0000269|PubMed:11526005, ECO:0000269|PubMed:15774876};
CC -!- PATHWAY: Aromatic compound metabolism. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; U78099; AAC46395.1; -; Genomic_DNA.
DR SMR; O69264; -.
DR BioCyc; MetaCyc:MON-14401; -.
DR BRENDA; 1.3.1.119; 16224.
DR GO; GO:0016628; F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR017711; BphB_TodD.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR TIGRFAMs; TIGR03325; BphB_TodD; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 1: Evidence at protein level;
KW Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.
FT CHAIN 1..275
FT /note="Chlorobenzene dihydrodiol dehydrogenase"
FT /id="PRO_0000453519"
FT ACT_SITE 155
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
SQ SEQUENCE 275 AA; 28610 MW; A3408B37D179F56C CRC64;
MKLKGEVALV TGGGAGLGRA IVDRYVAEGA RVAVLDKSAA GLEEIRKRHG DAVVGIEGDV
RSLDSHREAV ARCVETFGKL DCLIGNAGVW DYQTQLADIP DNGISEAFDE MFAIIVKGYI
LAAKAALPAL YKSKGSAIFT VSNAGFYPGG GGVLYTAGKH AVIGLVKQLA HEWGPRIRVN
GIAPGGILGS DIRGLKTLGL QDQTIATMPL ADMLGPVLPT GRVATAEEYA GAYVFFATRA
DTVPLTGSVL NIDGGMGVRG LFEASLGAQL DKHFA