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TECB_CUPXP
ID   TECB_CUPXP              Reviewed;         275 AA.
AC   O69264;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Chlorobenzene dihydrodiol dehydrogenase {ECO:0000303|PubMed:11526005};
DE            EC=1.3.1.119 {ECO:0000269|PubMed:11526005, ECO:0000269|PubMed:15774876};
GN   Name=tecB {ECO:0000303|PubMed:9249026};
OS   Cupriavidus sp. (strain PS12).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus; unclassified Cupriavidus.
OX   NCBI_TaxID=393999;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PS12;
RX   PubMed=9249026; DOI=10.1111/j.1432-1033.1997.00190.x;
RA   Beil S., Happe B., Timmis K.N., Pieper D.H.;
RT   "Genetic and biochemical characterization of the broad spectrum
RT   chlorobenzene dioxygenase from Burkholderia sp. strain PS12--dechlorination
RT   of 1,2,4,5-tetrachlorobenzene.";
RL   Eur. J. Biochem. 247:190-199(1997).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=PS12;
RX   PubMed=11526005; DOI=10.1128/aem.67.9.4057-4063.2001;
RA   Pollmann K., Beil S., Pieper D.H.;
RT   "Transformation of chlorinated benzenes and toluenes by Ralstonia sp.
RT   strain PS12 tecA (tetrachlorobenzene dioxygenase) and tecB (chlorobenzene
RT   dihydrodiol dehydrogenase) gene products.";
RL   Appl. Environ. Microbiol. 67:4057-4063(2001).
RN   [3]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=PS12;
RX   PubMed=15774876; DOI=10.1128/jb.187.7.2332-2340.2005;
RA   Pollmann K., Wray V., Pieper D.H.;
RT   "Chloromethylmuconolactones as critical metabolites in the degradation of
RT   chloromethylcatechols: recalcitrance of 2-chlorotoluene.";
RL   J. Bacteriol. 187:2332-2340(2005).
CC   -!- FUNCTION: Can transform various dihydrodiols of chlorobenzenes and
CC       chlorotoluenes into the respective catechols.
CC       {ECO:0000269|PubMed:11526005, ECO:0000269|PubMed:15774876}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(1R,2R)-3-chlorocyclohexa-3,5-diene-1,2-diol + NAD(+) = 3-
CC         chlorocatechol + H(+) + NADH; Xref=Rhea:RHEA:57608,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:19981, ChEBI:CHEBI:27715,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.3.1.119;
CC         Evidence={ECO:0000269|PubMed:11526005, ECO:0000269|PubMed:15774876};
CC   -!- PATHWAY: Aromatic compound metabolism. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; U78099; AAC46395.1; -; Genomic_DNA.
DR   SMR; O69264; -.
DR   BioCyc; MetaCyc:MON-14401; -.
DR   BRENDA; 1.3.1.119; 16224.
DR   GO; GO:0016628; F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR017711; BphB_TodD.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR03325; BphB_TodD; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.
FT   CHAIN           1..275
FT                   /note="Chlorobenzene dihydrodiol dehydrogenase"
FT                   /id="PRO_0000453519"
FT   ACT_SITE        155
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
SQ   SEQUENCE   275 AA;  28610 MW;  A3408B37D179F56C CRC64;
     MKLKGEVALV TGGGAGLGRA IVDRYVAEGA RVAVLDKSAA GLEEIRKRHG DAVVGIEGDV
     RSLDSHREAV ARCVETFGKL DCLIGNAGVW DYQTQLADIP DNGISEAFDE MFAIIVKGYI
     LAAKAALPAL YKSKGSAIFT VSNAGFYPGG GGVLYTAGKH AVIGLVKQLA HEWGPRIRVN
     GIAPGGILGS DIRGLKTLGL QDQTIATMPL ADMLGPVLPT GRVATAEEYA GAYVFFATRA
     DTVPLTGSVL NIDGGMGVRG LFEASLGAQL DKHFA
 
 
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