TECT_DROME
ID TECT_DROME Reviewed; 657 AA.
AC Q9VMH0; Q2MV53; Q8MSM0;
DT 04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 4.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Tectonic {ECO:0000303|PubMed:12537572, ECO:0000312|FlyBase:FBgn0261697};
DE AltName: Full=dTectonic;
DE Flags: Precursor;
GN Name=tctn {ECO:0000312|FlyBase:FBgn0261697};
GN ORFNames=CG9227 {ECO:0000312|FlyBase:FBgn0261697};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=16357211; DOI=10.1101/gad.1363606;
RA Reiter J.F., Skarnes W.C.;
RT "Tectonic, a novel regulator of the Hedgehog pathway required for both
RT activation and inhibition.";
RL Genes Dev. 20:22-27(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Testis;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, IDENTIFICATION IN THE MKS COMPLEX, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=27646273; DOI=10.1083/jcb.201603086;
RA Vieillard J., Paschaki M., Duteyrat J.L., Augiere C., Cortier E.,
RA Lapart J.A., Thomas J., Durand B.;
RT "Transition zone assembly and its contribution to axoneme formation in
RT Drosophila male germ cells.";
RL J. Cell Biol. 214:875-889(2016).
CC -!- FUNCTION: Probable component of the tectonic-like complex (also named
CC MKS complex), a complex localized at the transition zone of primary
CC cilia (PubMed:27646273). Has a role in ciliary structure and function
CC (PubMed:27646273). {ECO:0000269|PubMed:27646273}.
CC -!- SUBUNIT: Probable component of the tectonic-like complex (also named
CC MKS complex), composed of B9d1, B9d2, Cc2d2a, Mks1 and tctn.
CC {ECO:0000305|PubMed:27646273}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}. Cytoplasm, cytoskeleton, cilium basal
CC body {ECO:0000269|PubMed:27646273}. Cytoplasm, cytoskeleton,
CC microtubule organizing center, centrosome, centriole
CC {ECO:0000269|PubMed:27646273}. Note=Localizes at the transition zone, a
CC region between the basal body and the ciliary axoneme
CC (PubMed:27646273). Co-localizes with the tectonic-like complex
CC (PubMed:27646273). {ECO:0000269|PubMed:27646273}.
CC -!- TISSUE SPECIFICITY: Expressed in chordotonal neurons in the antennae
CC and male germ cells (at protein level). {ECO:0000269|PubMed:27646273}.
CC -!- DISRUPTION PHENOTYPE: Simultaneous knockout of B9d2 and tctn is viable
CC and does not show sensory behavioral defects (PubMed:27646273). Males
CC produce motile sperm and show only slightly reduced fertility
CC (PubMed:27646273). Sperm flagella show weak ultrastructural defects
CC including broken and disorganised structure (PubMed:27646273). Length
CC of the transition zone is decreased and recruitment of the tectonic-
CC like complex compromised (PubMed:27646273).
CC {ECO:0000269|PubMed:27646273}.
CC -!- SIMILARITY: Belongs to the tectonic family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM50568.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR EMBL; DQ278873; ABB90565.1; -; mRNA.
DR EMBL; AE014134; AAF52348.3; -; Genomic_DNA.
DR EMBL; AY118708; AAM50568.1; ALT_TERM; mRNA.
DR RefSeq; NP_608998.2; NM_135154.3.
DR AlphaFoldDB; Q9VMH0; -.
DR IntAct; Q9VMH0; 1.
DR STRING; 7227.FBpp0292198; -.
DR GlyGen; Q9VMH0; 10 sites.
DR PaxDb; Q9VMH0; -.
DR PRIDE; Q9VMH0; -.
DR EnsemblMetazoa; FBtr0303079; FBpp0292198; FBgn0261697.
DR GeneID; 33866; -.
DR KEGG; dme:Dmel_CG42731; -.
DR UCSC; CG9227-RA; d. melanogaster.
DR CTD; 33866; -.
DR FlyBase; FBgn0261697; tctn.
DR VEuPathDB; VectorBase:FBgn0261697; -.
DR eggNOG; KOG4028; Eukaryota.
DR HOGENOM; CLU_018942_0_0_1; -.
DR InParanoid; Q9VMH0; -.
DR OMA; DFQYNHG; -.
DR PhylomeDB; Q9VMH0; -.
DR BioGRID-ORCS; 33866; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 33866; -.
DR PRO; PR:Q9VMH0; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0261697; Expressed in testis and 2 other tissues.
DR Genevisible; Q9VMH0; DM.
DR GO; GO:0005814; C:centriole; IEA:UniProtKB-SubCell.
DR GO; GO:0035869; C:ciliary transition zone; IDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005615; C:extracellular space; ISS:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0036038; C:MKS complex; ISS:FlyBase.
DR GO; GO:0060271; P:cilium assembly; IBA:GO_Central.
DR GO; GO:0007288; P:sperm axoneme assembly; IMP:FlyBase.
DR InterPro; IPR040354; Tectonic.
DR InterPro; IPR011677; Tectonic_dom.
DR PANTHER; PTHR14611; PTHR14611; 1.
DR Pfam; PF07773; DUF1619; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium biogenesis/degradation; Cytoplasm; Cytoskeleton;
KW Glycoprotein; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..657
FT /note="Tectonic"
FT /id="PRO_0000229803"
FT TOPO_DOM 20..629
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 630..650
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 651..657
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 35..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 98
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 103
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 289
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 331
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 349
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 385
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 413
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 482
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 502
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 6
FT /note="P -> L (in Ref. 1; ABB90565 and 4; AAM50568)"
FT /evidence="ECO:0000305"
FT CONFLICT 11
FT /note="C -> F (in Ref. 1; ABB90565 and 4; AAM50568)"
FT /evidence="ECO:0000305"
FT CONFLICT 321..323
FT /note="NKS -> DKA (in Ref. 1; ABB90565 and 4; AAM50568)"
FT /evidence="ECO:0000305"
FT CONFLICT 341
FT /note="H -> Q (in Ref. 1; ABB90565 and 4; AAM50568)"
FT /evidence="ECO:0000305"
FT CONFLICT 384
FT /note="L -> P (in Ref. 1; ABB90565 and 4; AAM50568)"
FT /evidence="ECO:0000305"
FT CONFLICT 390
FT /note="K -> N (in Ref. 1; ABB90565 and 4; AAM50568)"
FT /evidence="ECO:0000305"
FT CONFLICT 426
FT /note="L -> S (in Ref. 1; ABB90565 and 4; AAM50568)"
FT /evidence="ECO:0000305"
FT CONFLICT 462
FT /note="I -> V (in Ref. 1; ABB90565 and 4; AAM50568)"
FT /evidence="ECO:0000305"
FT CONFLICT 501
FT /note="H -> D (in Ref. 1; ABB90565 and 4; AAM50568)"
FT /evidence="ECO:0000305"
FT CONFLICT 508
FT /note="D -> A (in Ref. 1; ABB90565 and 4; AAM50568)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 657 AA; 73933 MW; 2CC74D54592EABB0 CRC64;
MKEVLPLVVL CLATYHTHSI KIGISHNYNA TISPTASTTT EAPATTGAPS SETVSSISTL
PEAEVTTTTP AITFPPRVQQ KPKVAVASPT MSTALPLNST EVNSTLSVSG DNTFYYCSCD
LQAGRCDLNC CCDNDCPLET RQVFNCLPNS LLPQLESRLE DFQYTHGLPT CQINDGWLCV
FRSNTKATKT QPPDMNIDTS QYRKWKDNLE YQESDYAQSR PSAGHYKFGQ TLQLWQPETK
QLATLELPAA YESPNCQLKQ SVWHLQPIRH VCRMKDSAQL QESIWSLLNQ TSNYEILSKP
RDLEEPEVNG LIVQVCLRGA NKSMRCLERG NDTQLDVIVD HVELQLIHNF TNILEAKLFL
EEAKLAEDDN EPLWLRYNVE FVTLNESLTK PTSGPLGYLS GAPVILSRML PQNSSEDKQL
ISYHSLNQNI KEFHWLSLPS RKPRGSSCQR ALDHKEALRF GIDLLTRCEL RHAAPLLQAH
ANHTEYCQGL QAQIWSLLLP HNCTQLEDVT KVFVSHLGRP QPDKWLPMEV RYPENVHEMP
PPVQAVYDEM RQSLSCRNIF LSVGYEFHVA HLAMVEGRAP HQRVLQHARL VLGQRHDLEF
DTSEIEVALP LSISAMFYRM QTKALSNGAA VGIAGHLVLV EMIYLGIIYL GSMQLCL
