ID   BRAB_ANNTR              Reviewed;         421 AA.
AC   P9WER1; A0A866WL92;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   25-MAY-2022, entry version 3.
DE   RecName: Full=O-glycosyltransferase braB {ECO:0000303|PubMed:32936132};
DE            EC=2.4.1.- {ECO:0000269|PubMed:32936132};
DE   AltName: Full=Brasilane terpene glycosides biosynthesis cluster protein B {ECO:0000303|PubMed:32936132};
GN   Name=braB {ECO:0000303|PubMed:32936132};
OS   Annulohypoxylon truncatum (Hypoxylon truncatum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Xylariomycetidae; Xylariales; Hypoxylaceae; Annulohypoxylon.
OX   NCBI_TaxID=327061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP   PATHWAY.
RC   STRAIN=DSM 103480 / CBS 140778;
RX   PubMed=32936132; DOI=10.1039/d0cc03950k;
RA   Feng J., Surup F., Hauser M., Miller A., Wennrich J.P., Stadler M.,
RA   Cox R.J., Kuhnert E.;
RT   "Biosynthesis of oxygenated brasilane terpene glycosides involves a
RT   promiscuous N-acetylglucosamine transferase.";
RL   Chem. Commun. (Camb.) 56:12419-12422(2020).
CC   -!- FUNCTION: O-glycosyltransferase; part of the gene cluster that mediates
CC       the biosynthesis of the brasilane terpene glycosides brasilane D and E
CC       (PubMed:32936132). The biosynthesis starts with the activity of the
CC       terpene cyclase braA that converts farnesyl pyrophosphate into the
CC       sesquiterpene alcohol trichobrasilenol (PubMed:32936132). Subsequently,
CC       trichobrasilenol is glycosylated by the O-glycosyltransferase braB
CC       putatively using UDP-GlcNAc as sugar donor to yield brasilane A
CC       (PubMed:32936132). The latter then undergoes two rounds of oxidation
CC       performed by the cytochrome P450 monooxygenase braC (PubMed:32936132).
CC       In the first round braC hydroxylates C-12 forming brasilane D, which
CC       serves as substrate in the second round to establish the epoxide at the
CC       bond between C-5 and C-10 and oxidize the alcohol at C-12 to an
CC       aldehyde leading to the final product brasilane E (PubMed:32936132).
CC       BraB is also able to glycosylate geraniol, linalool, perillyl alcohol,
CC       3,4-dichlorophenol and, to a lesser extend, benzyl alcohol
CC       (PubMed:32936132). {ECO:0000269|PubMed:32936132}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:32936132}.
CC   -!- SIMILARITY: Belongs to the afumC glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; MT383109; QOE88884.1; -; Genomic_DNA.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR008441; AfumC-like_glycosyl_Trfase.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF05704; Caps_synth; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Transferase.
FT   CHAIN           1..421
FT                   /note="O-glycosyltransferase braB"
FT                   /id="PRO_0000453908"
FT   REGION          1..26
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   421 AA;  47903 MW;  D538DDD5CC8BF02A CRC64;
     MVPSVMEGAP QLGITSTDTS SAGVPPGLKL IPTDRLDRRT DEEIAAWLQT RHPVTSDKNV
     WAFWHNGYTN MPPWVQRNII NWVRRLGPDW TVHLLDRVDG SATNVSHYVD SSFFPECFNN
     NTMDGPTVGQ HSGDLVRLPL LWLYGGIWID AGSFLFRHVE DICWNKIEDP ESPYEMAGFV
     IEMRPGVEVM LNGFIAAKRG NPFIKRWLEI FKKLWDGATN VQGFHKHPLL RHLPMLCPPI
     DKLNLPPQGL NVVMEQFTDY MSQIMSFERL RKLVDPSDGF NGPEYYSNKM LLCSALQETF
     YFQLVTEWSG TKQFNLLSTK RKGEGVVKDE NWHAAENFVH DALANTATMK LSHGPPGALD
     SFLADLWDSE EHHGKDNEDG TFAAYLRYGS VHFDQTREMV PIKMGWPDEE VLEAGVLEPK
     K